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作物学报 ›› 2014, Vol. 40 ›› Issue (02): 222-230.doi: 10.3724/SP.J.1006.2014.00222

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甜菜遗传连锁图谱初步构建

王茂芊1,2,3,李博1,王华忠1,2,3,*   

  1. 1黑龙江省普通高校甜菜遗传育种重点实验室 / 黑龙江大学农作物研究院, 黑龙江哈尔滨 150080；2中国农业科学院甜菜研究所, 黑龙江哈尔滨 150080；3中国农业科学院北方糖料作物资源与利用重点开放实验室, 黑龙江哈尔滨 150080
  • 收稿日期:2013-03-11 修回日期:2013-09-06 出版日期:2014-02-12 网络出版日期:2013-11-14
  • 通讯作者: 王华忠, E-mail: wwhhzz0451@sohu.com, Tel: 0451-86609494
  • 基金资助:

    本研究由国家自然科学基金项目(31271780), 国家现代农业产业技术体系建设专项(CARS-210104)和黑龙江大学青年基金(QL201038)项目资助。

Construction of Molecular Genetic Linkage Map of Sugarbeet

WANG Mao-Qian1,2,3,LI Bo1,WANG Hua-Zhong1,2,3,*   

  1. 1 Key Laboratory of Sugar Beet Genetic Breeding/ Crop Academy of Heilongjiang University, Harbin150080, China; 2 Sugar Beet Research Institute, Chinese Academy of Agricultural Sciences, Harbin150080, China; 3 Key Laboratory of North Sugar Crop Resource and Utilization, Chinese Academy of Agricultural Sciences,Harbin 150080, China
  • Received:2013-03-11 Revised:2013-09-06 Published:2014-02-12 Published online:2013-11-14
  • Contact: 王华忠, E-mail: wwhhzz0451@sohu.com, Tel: 0451-86609494

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摘要：

以甜菜高产低糖型JV34-2和低产高糖型2B023两材料杂交, 构建了200个单株的F2作图群体, 利用所筛选出的56对SRAP引物组合和20对SSR引物, 对F2作图群体进行PCR扩增和遗传连锁分析, 初步构建了一张包含9个连锁群、141个(123个SRAP和18个SSR)标记位点的甜菜遗传连锁图谱。该图谱覆盖长度为1399.88 cM, 平均图距9.92 cM。未进入连锁群的有4个标记。9个连锁群包含3~26个标记不等, 连锁群遗传距离15.69~237.21 cM。连锁群上有20.56%的标记出现偏分离, 主要集中在Ch3连锁群上, 其余分散在Ch1、Ch2、Ch8和Ch9中。该图谱是我国甜菜领域利用SRAP和SSR相结合方法, 构建的第一个较精密的分子遗传图谱, 为重要性状的基因定位和优良基因的克隆奠定了基础。

关键词: 甜菜, SRAP, SSR, 遗传图谱

Abstract:

Molecular genetic map is a fundamental tool for genomic research. In this study, a molecular genetic map was constructed using an F2 population consisting of 200 individuals from a cross between sugarbeet JV34-2 (the high beet yield and low sugar content line) and sugarbeet 2B023 (the low beet yield and high sugar content line) and a total of 561 SRAP primers and 114 SSR primers. Approximately 56 out of 561 and 20 out of 114 primers, respectively, showed polymorphisms. Each of these polymorphic primers produced at least one scorable polymorphic DNA band which was visible enough for detection and scoring. The map consisted of nine linkage groups which included 141 (123 SRAP and 18 SSR) markers, and covered 1399.88 cM with an average distance of 9.92 cM. Three markers were still unlinked. All nine linkage groups consisting of 3–26 markers were 15.69–237.21 cM in length. Twenty point five six percent partially segregated markers distributed in the map, were mainly located on Ch3 linkage groups, and the others were mapped on Ch1, Ch2, Ch8, and Ch9. This is the first gene map of sugarbeet, constructed by SRAP and SSR markers in China. It will provide the basis for gene mapping of important characters and cloning of excellent gene in sugarbeet.

Key words: Sugarbeet, SRAP, SSR, Genetic linkage map

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