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作物学报 ›› 2014, Vol. 40 ›› Issue (05): 798-804.doi: 10.3724/SP.J.1006.2014.00798

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦品种汶农14抗白粉病基因的染色体定位

宋伟1,2,**,孙会改1,2,**,孙艳玲2,赵紫慧1,2,王晓鸣2,武小菲2,李洪杰2,*   

  1. 1 河北科技师范学院生命科技学院, 河北秦皇岛066604; 2 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程, 北京100081
  • 收稿日期:2013-12-31 修回日期:2014-03-04 出版日期:2014-05-12 网络出版日期:2014-03-24
  • 通讯作者: 李洪杰, E-mail: lihongjie@caas.cn
  • 基金资助:

    本研究由国家现代农业产业体系建设专项(CARS-3-1)和国家转基因生物新品种培育重大专项(2014ZX0800906B-003)资助。

Wheat|Blumeria graminis f. sp. tritici|Resistance gene|Pm2|Molecular marker

SONG Wei1,2,SUN Hui-Gai1,2,SUN Yan-Ling2,ZHAO Zi-Hui1,2,WANG Xiao-Ming2,WU Xiao-Fei2,LI Hong-Jie2,*   

  1. 1 College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-12-31 Revised:2014-03-04 Published:2014-05-12 Published online:2014-03-24

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摘要：

汶农14是近年山东省和国家审定一个半冬性小麦品种。采用来自不同地区的52个小麦白粉菌菌株对汶农14进行抗性鉴定，并利用分子标记分析定位了其抗白粉病基因。汶农14对43个菌株(82.7%)表现抗性反应型，对9个菌株表现感病反应型。这些菌株对汶农14的毒力谱与已知抗白粉病基因Pm2相似，但汶农14对11个菌株的反应与携带Pm2的Ulka/8*Cc不同。此外，利用26个菌株的鉴定结果表明，汶农14与携带Pm46的Tabasco相比，与3个菌株的反应型表现不同。汶农14在成株期对白粉病混合菌株表现高抗。利用汶农14×邯4564的F2和F2:3群体进行遗传分析，发现汶农14对E09菌株的抗性受1对显性基因控制，暂命名为PmW14。分子标记分析显示，PmW14与Xcfd8、Xcfd81和SCAR203连锁，遗传距离分别为7.5、1.8和7.7 cM。由于这些分子标记被定位于小麦5DS染色体的5DS-1-0-0.63区间，且与Pm2基因紧密连锁，因此推测，PmW14可能与Pm2位于相同的基因座。

关键词: 小麦, 白粉菌, 抗病基因, Pm2, 分子标记

Abstract:

Wennong 14 is a facultative wheat cultivar commercialized in Shandong province and the neighbouring provinces in the northern part of Yellow and Huai River Valleys Facultative Wheat Zone in China. In this study, an array of Blumeria graminis f. sp. tritici (Bgt) isolates were used to test the resistance of Wennong 14 to powdery mildew at both seedling and adult stages. Among the 52 Bgt isolates tested at seedling stage, Wennong 14 was resistant to 43 and susceptible to 9 isolates. The virulence pattern of these Bgt isoaltes on Wennong 14 was similar as that of the known powdery mildew resistance gene Pm2, but the reactions of Wennong 14 to 11 Bgt isolates differed from those of Ulka/8*Cc carrying Pm2. Additionally, Wennong 14 was different from Tabasco carrying Pm46 in the reaction to three isoaltes when tested with 26 Bgt isolates. Wennong 14 was highly resistant to a mixture of Bgt isolates at adult stage. Using segregation populations of F2 and F2:3 developed from the cross of Wennong 14 × Han 4564, genetic analysis demonstrated that the resistance against Bgt isolate E09 was controlled by a single dominant gene, designated PmW14. Based on the results of molecular analysis, PmW14 was linked to markers Xcfd8, Xcfd81, and SCAR203, with genetic distances of 7.5, 1.8, and 7.7 cM, respectively. Because these markers were previously localized on wheat chromosome 5DS in the negion of 5DS-1-0-0.63 and linked to gene Pm2, PmW14 was most likely located on this locus and to be either the same as or an allele of Pm2.

Key words: Wheat, Blumeria graminis f. sp. tritici, Resistance gene, Pm2, Molecular marker

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