亚洲棉和雷蒙德氏棉PEBP家族基因的鉴定及该家族基因在陆地棉组织中表达分析

doi:10.3724/SP.J.1006.2015.00394

摘要/Abstract

摘要：

磷脂酰乙醇胺结合蛋白(phosphatidyl ethanolamine-binding proteins, PEBP)基因家族广泛存在于真核生物中，在被子植物中主要起着促进或抑制开花和控制株型的作用。利用亚洲棉(Gossypium arboreum, A₂)和雷蒙德氏棉(Gossypium raimondii, D₅)的基因组数据库，分别搜索到8个棉花PEBP同源基因，都包含4个外显子和3个内含子，编码的蛋白都存在PEBP家族的保守基序和关键氨基酸位点，表明二倍体棉花中至少存在8个PEBP家族基因。进化分析表明，8个PEBP基因分属于3个亚家族，含FLOWERING LOCUS T (FT)-like亚家族1个、TERMINAL FLOWER 1 (TFL1)-like亚家族5个(包括3个TFL1和2个BFT)、MOTHER OF FT AND TFL1 (MFT)-like亚家族2个。实时荧光定量PCR分析陆地棉(Gossypium hirsutum) 8个PEBP基因在根、茎、叶、幼苗顶端分生组织、花、胚珠和25 d的纤维组织中的表达，表明FT1在叶片中表达量最高，其次是纤维、胚珠和花中；MFT1在各组织中均表达，但在纤维中表达量最高，其次是花和叶片中，而MFT2以在叶片中表达为主；TFL1a、TFL1b和TFL1c均在根中表达量最高，但TFL1c在叶片、花和胚珠中也有相对较高的表达；BFT1和BFT2在叶片中表达量最高，但除幼苗顶端分生组织外，BFT1在其他各组织中的表达明显高于BFT2。这些结果表明，PEBP家族基因在棉花的生长发育中可能具有不同的功能。

关键词: 磷脂酰乙醇胺结合蛋白, 亚洲棉, 雷蒙德氏棉, 成花素, 基因表达

Abstract:

The phosphatidylethanolamine-binding proteins (PEBP) widely exist in eukaryotes. In angiosperms, PEBP family genes play important roles in promoting or inhibiting flowering, as well as plant architecture control. Eight PEBP genes were identified from diploid cotton Gossypium arboreum (A₂) and Gossypium raimondii (D₅) genome database, respectively. All the PEBP genes of cotton contained four exons and three introns, and their encoded proteins contained a conserved PEBP motif and critical amino acid sites of PEBP family, which indicated there were at least eight PEBP genes in diploid cotton. Phylogenetic analysis showed that eight cotton PEBP genes comprised three subfamilies: FLOWERING LOCUS T (FT)-like containing one gene, TERMINAL FLOWER 1 (TFL1)-like containing five genes including three TFL1 and two BFT genes, and MOTHER OF FT AND TFL1 (MFT)-like containing two genes. The expression patterns of eight Gossypium hirsutum PEBP family genes in root, stem, leaf, shoot apical meristem, flower, ovule and 25 days post-anthesis (DPA) fiber were identified with quantitative Real-time reverse transcription PCR (qRT-PCR). The results showed that FT1 transcript was preferentially expressed in leaf and secondly in fiber, ovule and flower. MFT1 expressed in all the tissues, with the highest expression level in fiber, then in flower and leaf, while MFT2 transcript was preferentially expressed in leaf. TFL1a, TFL1b and TFL1c expressed mainly in root, and TFL1c also expressed in leaf, flower and ovule. Expression of BFT1 and BFT2 were present mainly in leaf, and that of BFT1 in the other six tissues except in shoot apical meristem (SAM) was higher than that of BFT2. Expression analysis revealed that eight PEBP genes in cotton have different expression patterns, showing their different functional roles in regulation of cotton development.

Key words: PEBP, Gossypium arboreum, Gossypium raimondii, Florigen, Gene expression

李超，张彦楠，刘焕龙，黄先忠. 亚洲棉和雷蒙德氏棉PEBP家族基因的鉴定及该家族基因在陆地棉组织中表达分析[J]. 作物学报, 2015, 41(03): 394-404.

LI Chao,ZHANG Yan-Nan,LIU Huan-Long,HUANG Xian-Zhong. Identification of PEBP Gene Family in Gossypium arboreum and Gossypium raimondii and Expression Analysis of the Gene family in Gossypium hirsutum[J]. Acta Agron Sin, 2015, 41(03): 394-404.

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