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作物学报 ›› 2015, Vol. 41 ›› Issue (03): 405-413.doi: 10.3724/SP.J.1006.2015.00405

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜及其亲本物种甲基化酶I基因的克隆及表达模式

谭河林1,许欣颖1，付立曼1，向小娥2，李剑桥1，郭昊伦1，叶文雪1   

  1. 1南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京210095；2南京农业大学动物科学类国家教学示范中心，江苏南京210095
  • 收稿日期:2014-09-03 修回日期:2014-12-19 出版日期:2015-03-12 网络出版日期:2015-01-12
  • 基金资助:

    本研究由江苏省自然科学基金项目(BK2012767)和中央高校基本科研业务费专项(KYZ201301)资助。

Cloningand Expression Pattern of DNA Methylase I (MET1) from Brassica napus L. and Its Progenitors

TAN He-Lin1,*,XU Xin-Ying1,FU Li-Man1,XIANG Xiao-E2,LI Jian-Qiao1,GUO Hao-Lun1,YE Wen-Xue1   

  1. 1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China;2Animal Sciences National Teaching Demonstration Center,Nanjing Agricultural University, Nanjing 210095, China
  • Received:2014-09-03 Revised:2014-12-19 Published:2015-03-12 Published online:2015-01-12

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摘要：

DNA甲基化作为一种表观遗传调控方式，在植物体生长发育中起重要作用，而甲基转移酶I (DNA methylase I, MET1)在甲基化过程中起主要作用。本研究克隆到5个油菜BnMET1同源基因，并比较白菜BrMET1、甘蓝BoMET1和油菜BnMET1同源基因的进化以及其表达模式。结果显示白菜和甘蓝MET1同源基因在进化中较为保守，而油菜BnMET1基因结构发生较大改变；部分油菜BnMET1同源基因表达发生沉默，而激活表达的BnMET1表达模式较其亲本白菜和甘蓝直系MET1同源基因发生明显的改变。上述结果表明BnMET1同源基因通过改变基因结构以及表达模式影响油菜组织中BnMET1基因剂量，从而调节油菜的生长发育。

关键词: DNA甲基化, 甲基化酶I, 基因进化, 基因差异表达

Abstract:

Methylation in genomic DNA, a way of epigenetic regulation, plays animportant role during the growth and development of plant. As allotetraploid oilseeds plant, Brassica napus (AACC, 2n=38) originated from the natural cross between Brassica rapa (AA, 2n=20) and Brassica oleracea (CC, 2n=18), containing duplicate genes. Here, we isolated and characterized five Brassica napus BnMET1s, which are orthologous gene of Arabidopsis AtMET1 acted as transferase in DNA methylation. Our results showed that there were conspicuous variations in gene structuresof BnMET1s compared with its orthologos of BrMET1 and BoMET1, leading to more abundant divergences in coding region of BnMET1s. Moreover, we found that some divergences among BnMET1paralogous genes were derived from its progenitor orthologousgenes of Brassica rapa or Brassica oleracea. Furthermore, the transcription analysis indicated that partialBnMET1paralogs were silence,and the expression patternsof the activated BnMET1were altered in contrast to its BoMET1 orthologs in Brassica oleracea and BrMET1 orthologs in Brassica rapa. Taken all these together, we speculated that duplicate BnMET1s regulate the development process ofBrassica napuswitha certain gene dosagekept by alteringtheir gene structures and spatio-temporal expression patterns.

Key words: DNA methylation, MET1 geneGene evolution, Differential expression

[1]Finnegan E J, Genger R K, Peacock W J, Dennis E S. DNA methylation in plants. Annu Rev Plant Physiol Plant Mol Biol, 1998, 49: 223–247

[2]Saze H, Sasaki T, Kakutani T. Negative regulation of DNA methylation in plants. Epigenetics, 2008, 3: 122–124

[3]Teixeira F K, Colot V. Gene body DNA methylation in plants: a means to an end or an end to a means? EMBO J, 2009, 28: 997–998

[4]Finnegan E J, Kovac K A, Jaligot E, Sheldon C C, Peacock W J, Dennis E S. The downregulation of FLOWERING LOCUS C (FLC) expression in plants with low levels of DNA methylation and by vernalization occurs by distinct mechanisms. Plant J, 2005, 44: 420–432

[5]Vanyushin B F, Kirnos M D. DNA methylation in higher plants: past, present and future. Biochim Biophys Acta, 2011, 1809: 360–368

[6]Vanyushin B F. DNA methylation in plants. Curr Top Micro-biol Immunol, 2006, 301: 67–122

[7]Meyer P. DNA methylation systems and targets in plants. FEBS Lett, 2011, 585: 2008–2015

[8]Law J A, Jacobsen S E. Establishing, maintaining and modi-fying DNA methylation patterns in plants and animals. Nat Rev Genet, 2010, 11: 204–220

[9]夏晗, 刘美芹, 尹伟伦, 卢存福, 夏新莉. 植物DNA甲基化调控因子研究进展. 遗传, 2008, 30: 426–432

Xia H, Liu M Q, Yi W L, Lu C F, Xia X L. DNA methylation regulating factors in plants. Hereditas (Beijing), 2008, 30: 426–432 (in Chinese with English abstract)

[10]Finnegan E J, Peacock W J, Dennis E S. Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development. Proc Natl Acad Sci USA, 1996, 93: 8449–8454

[11]Apashkin V V, Kutueva L I, Vaniushin B F. Is the cytosine DNA methyltransferase gene MET1 regulated by DNA methylation in Arabidopsis thaliana plants? Genetika, 2011, 47: 320–331

[12]Kankel M W, Ramsey D E, Stokes T L, Flowers S K, Haag J R, Jeddeloh J A, Riddle N C, Verbsky M L, Richards E J. Arabidopsis MET1 cytosine methyltransferase mutants. Genetics, 2003, 163: 1109–1122

[13]Soppe W J, Jacobsen S E, Alonso-Blanco C, Jackson J P, Kakutani T, Koornneef M, Peeters A J. The late flowering phenotype of fwa mutants is caused by gain-of-function epigenetic alleles of a homeodomain gene. Mol Cell, 2000, 6: 791–802

[14]闫延涛. 普通小麦中春化基因VRN1及甲基转移酶基因的克隆及表达. 河南农业大学硕士学位论文, 河南郑州 2010

Yan Y T. Cloning and expression of the vernalization gene VRN1 and DNA methyltransferase in Wheat. MS Thesis of Henan              Agricultural University, Zhengzhou, China, 2010 (in Chinese with English abstract)

[15]Nakano Y, Steward N, Sekine M, Kusano T, Sano H. A tobacco NtMET1 cDNA encoding a DNA methyltransferase: molecular characterization and abnormal phenotype of transgene tobacco plants. Plant Cell Physiol, 2000, 41: 448–457

[16]Chang L, Zhang Z, Han B, Li H, Dai H, He P, Tian H. Isolation of DNA methyltransferase genes from strawberry (Fragaria × ananassa Duch.) and their expression in relation to micropropagation. Plant Cell Rep, 2009, 28: 1373–1384

[17]Steward N, Kusano T, Sano H. Expression of ZmMET1, a gene encoding a DNA methyltransferase from maize, is associated not only with DNA replication in actively proliferating cells but also with altered DNA methylation status in cold-stressed quiescent cells. Nucl Acids Res, 2000, 28: 3250–3259

[18]Zhang T, Zhao X, Wang W, Pan Y, Huang L, Liu X, Zong Y, Zhu L, Yang D, Fu B. Comparative transcriptome profiling of chilling stress responsiveness in two contrasting rice genotypes. PLoS One, 2012, 7: e43274



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