作物学报 ›› 2015, Vol. 41 ›› Issue (03): 479-486.doi: 10.3724/SP.J.1006.2015.00479
何海旺,潘华清,张铙丹,何龙飞*
HE Hai-Wang,PAN Hua-Qing,ZHANG Nao-Dan,HE Long-Fei*
摘要:
利用RT-PCR和RACE技术从飞机草中克隆得到1628 bp的类黄酮3’-羟化酶cDNA的全长序列,命名为CoF3’H,GenBank登录号为HQ268505.1。CoF3’H基因的编码区长度为1524 bp,编码507个氨基酸。氨基酸序列含P450蛋白结构域和半胱氨酸亚铁血红素结合域保守区(F××G×R×C×G),利用DNAMAN软件比对显示,CoF3’H与其他植物的F3’H蛋白的同源性很高。通过农杆菌介导成功地将CoF3’H基因导入烟草,T2代的转CoF3’H基因烟草植株的黄酮含量显著高于野生型。说明CoF3¢H在黄酮的生物合成中起重要作用,为后续研究飞机草的化感作用及综合利用提供基础。
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