作物学报 ›› 2015, Vol. 41 ›› Issue (04): 515-523.doi: 10.3724/SP.J.1006.2015.00515
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
王仲怡1,付海宁1,2,孙素丽1,段灿星1,武小菲1,杨晓明2,朱振东1,*
WANG Zhong-Yi1,FU Hai-Ning1,2,SUN Su-Li1,DUAN Can-Xin1,WU Xiao-Fei1,YANG Xiao-Ming2,ZHU Zhen-Dong1,*
摘要:
白粉病是豌豆的主要病害之一,在全球范围内引起严重经济损失。防治豌豆白粉病最有效、经济和环境友好的方法是利用抗病品种。迄今,2个隐性抗白粉病基因er1、er2和一个显性抗白粉病基因Er3已在豌豆中被鉴定,其中er1基因在世界上被广泛应用于抗病品种培育。er1基因隶属MLO基因家族,其抗性由豌豆PsMLO1基因座位功能丧失产生。X9002是甘肃省农业科学院培育的一个半无叶(afila)抗白粉病豌豆品系。本研究对X9002抗白粉病基因进行鉴定,开发用于抗白粉病基因选择的分子标记。遗传分析表明X9002对白粉病抗性由一个隐性单基因控制,SSR标记将该基因定位到豌豆第VI连锁群er1座位区域,标记AD60和c5DNAmet与其连锁。PsMLO1基因序列分析发现,X9002存在一个未知大小和身份的片段插入,该类型突变也发生在含有er1-2等位基因的豌豆品种Stratagem和Franklin,表明X9002抗白粉病基因为er1-2。一个鉴定er1-2等位基因的功能标记PsMLO1-650被开发,该标记为互引相标记,仅在感病植株中扩增,可以有效用于分子辅助选择。
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