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作物学报 ›› 2015, Vol. 41 ›› Issue (04): 515-523.doi: 10.3724/SP.J.1006.2015.00515

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

豌豆品系X9002抗白粉病基因鉴定

王仲怡1,付海宁1,2,孙素丽1,段灿星1,武小菲1,杨晓明2,朱振东1,*   

  1. 1 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程,北京100081;2甘肃农业科学院,甘肃兰州 730070
  • 收稿日期:2014-10-25 修回日期:2015-02-06 出版日期:2015-04-12 网络出版日期:2015-03-03
  • 通讯作者: 朱振东, E-mail: zhuzhendong@caas.cn, Tel: 010-82109609
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-09),农业部作物种质资源保护子项目(NB2013-2130135-25-15),中国农业科学院科技创新工程,国家自然科学基金项目(31160304)项目资助。

Identification of Powdery Mildew Resistance Gene in Pea Line X9002

WANG Zhong-Yi1,FU Hai-Ning1,2,SUN Su-Li1,DUAN Can-Xin1,WU Xiao-Fei1,YANG Xiao-Ming2,ZHU Zhen-Dong1,*   

  1. 1 National Key Facility for Crop Gene Resource and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Gansu Academy of Agricultural Sciences, Lanzhou 730070, China
  • Received:2014-10-25 Revised:2015-02-06 Published:2015-04-12 Published online:2015-03-03
  • Contact: 朱振东, E-mail: zhuzhendong@caas.cn, Tel: 010-82109609

摘要:

白粉病是豌豆的主要病害之一,在全球范围内引起严重经济损失。防治豌豆白粉病最有效、经济和环境友好的方法是利用抗病品种。迄今,2个隐性抗白粉病基因er1、er2和一个显性抗白粉病基因Er3已在豌豆中被鉴定,其中er1基因在世界上被广泛应用于抗病品种培育。er1基因隶属MLO基因家族,其抗性由豌豆PsMLO1基因座位功能丧失产生。X9002是甘肃省农业科学院培育的一个半无叶(afila)抗白粉病豌豆品系。本研究对X9002抗白粉病基因进行鉴定,开发用于抗白粉病基因选择的分子标记。遗传分析表明X9002对白粉病抗性由一个隐性单基因控制,SSR标记将该基因定位到豌豆第VI连锁群er1座位区域,标记AD60和c5DNAmet与其连锁。PsMLO1基因序列分析发现,X9002存在一个未知大小和身份的片段插入,该类型突变也发生在含有er1-2等位基因的豌豆品种Stratagem和Franklin,表明X9002抗白粉病基因为er1-2。一个鉴定er1-2等位基因的功能标记PsMLO1-650被开发,该标记为互引相标记,仅在感病植株中扩增,可以有效用于分子辅助选择。

关键词: 豌豆, 白粉病, 抗病基因, PsMLO1基因, 功能标记

Abstract:

Powdery mildew is one of the major diseases in pea, causing severe economic loss worldwide. Planting resistant cultivars is the most effective, economical and eco-friendly method for controlling the disease. So far, two recessive resistance genes (er1, er2) and one dominant resistance gene (Er3) have been identified in pea, and er1 has been utilized in breeding programs worldwide. Gene er1 is a member of MLO gene family, and er1 resistance is caused by the loss of function at a PsMLO1 locus in pea. X9002 with resistance to powdery mildew is an afila pea line bred by Gansu Academy of Agricultural Sciences. Here, we identified the powdery mildew resistance gene in X9002, and developed molecular marker for the gene selection. Genetic analysis for powdery mildew resistance showed that X9002 carries a recessive resistance gene. The resistance gene was mapped in a region carrying er1 locus on the pea linkage group VI using SSR markers, and was linked to SSR marker AD60 and gene marker c5DNAmet. PsMLO1 sequence analysis revealed that X9002 carries an insertion of unknown size and identity. The same mutation also existed in pea cultivars Stratagem and Franklin carrying er1-2 allele, indicating that the resistance gene is er1-2 in X9002. A functional marker PsMLO1-650 for er1-2 was developed, and the marker was a coupling-phase marker that was detected only in susceptible plants. PsMLO1-650 can be used effectively in marker-assisted selection.

Key words: Pisum sativum L., Powdery mildew, Resistance gene, PsMLO1 gene, Functional marker

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