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作物学报

作物学报 ›› 2016, Vol. 42 ›› Issue (02): 295-302.doi: 10.3724/SP.J.1006.2016.00295

• 研究简报 • 上一篇    下一篇

两个谷子CIPK基因在非生物逆境胁迫下的表达分析

余爱丽1,2,赵晋锋1,2,王高鸿2,杜艳伟2,李颜方2,张正2,郭二虎2,梁爱华1,*   

  1. 1山西大学生物技术研究所, 山西太原030006；2山西省农业科学院谷子研究所 / 特色杂粮种质资源发掘与育种山西省重点实验室, 山西长治046011
  • 收稿日期:2015-07-20 修回日期:2015-11-20 出版日期:2016-02-12 网络出版日期:2015-12-07
  • 通讯作者: 梁爱华, E-mail: aliang@sxu.edu.cn
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-07-12.5-A10), 山西省生物工程重点实验室开放基金项目和山西省农业科学院科技自主创新能力提升工程项目(2015ZZCX-09)资助。

Expression Analysis of Two CIPK genes under Abiotic Stress in Foxtail Millet

U Ai-Li1,2,ZHAO Jin-Feng1,2,WANG Gao-Hong2,DU Yan-Wei2,LI Yan-Fang2,ZHANG Zheng2,GUO Er-Hu2,LIANG Ai-Hua1,*   

  1. 1Insitute of Biotechnology, Shanxi University, Taiyuan 030006, China; 2 Millet Research Institute, Shanxi Academy of Agricultural Sciences / Shanxi Key Laboratory of Genetic Resources and Breeding in Minor Crops, Changzhi 046011, China
  • Received:2015-07-20 Revised:2015-11-20 Published:2016-02-12 Published online:2015-12-07
  • Contact: 梁爱华, E-mail: aliang@sxu.edu.cn
  • Supported by:

    This study was supported by the grants from the Modern Agro-industry Technology System (CARS-07-12.5-A10), the Key Laboratory Open Fund Project of Bio-engineering in Shanxi Provincial, and the Enhance Technology Independent Innovation Ability Project in Shanxi Academy of Agricultural Sciences (2015ZZCX-09).

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摘要：

CIPK是一类丝氨酸/苏氨酸蛋白激酶，该蛋白在植物响应逆境胁迫过程中发挥重要作用。本文利用生物信息学方法从谷子基因组中鉴定出2个CIPK基因, 命名为SiCIPK6和SiCIPK16。序列分析表明SiCIPK6基因组序列长1994 bp，编码451个氨基酸；SiCIPK16基因组序列长1885 bp，编码473个氨基酸，2个基因均无内含子和可变剪切。生物信息学分析显示这2个基因在蛋白质序列和结构上与其他物种CIPK基因一样非常保守。实时定量PCR分析表明SiCIPK6和SiCIPK16在ABA、低温、高温、干旱、高盐诱导下表达量均有所上调, SiCIPK6基因在ABA、干旱和盐处理时表达量上调幅度较大，而SiCIPK16基因在低温、干旱和高温处理时表达量上调幅度较大。半定量PCR检测结果表明SiCIPK6和SiCIPK16两个基因在拔节、孕穗、灌浆期时均有表达，在相应生育时期受到干旱胁迫时它们表达量均有所提高。推测SiCIPK6和SiCIPK16基因在谷子的干旱或其他逆境胁迫中起一定作用。

关键词: 谷子, CIPK基因, 逆境

Abstract:

CIPK (CBL interacting protein kinase) is a type of serine or threonine protein kinases, which plays an important role in response to stress. In this study, we identified two CIPK genes designated as SiCIPK6 and SiCIPK16 from foxtail millet (Setaria italica) genome using bioinformatics methods. The sequence analysis showed that SiCIPK6 has a length of 1994 bp in the genome, encoding 513 amino acids residues, and SiCIPK16 is 1885 bp, encoding 473 amino acids residues. These two genes have no alternative splicing and intron. The characters predicted based on the bioinformatics analysis revealed that the protein sequences and structure of the two SiCIPK genes were very conservative just like CIPK genes in other species. Real-time PCR analysis discovered that the expression of SiCIPK6 and SiCIPK16 was up-regulated by ABA, cold, heat, drought and salt stress, respectively. The expression was strongly induced by ABA, drought and salt treatments for SiCIPK6, and by cold, drought and heat treatments for SiCIPK16. The semi-quantitative PCR analysis showed that SiCIPK6 and SiCIPK16 were expressed at the jointing, booting and filling stages, and induced by drought stress in the corresponding growth period. Foxtail millet CIPK genes reported in this study would enrich CIPK members in plant kingdom and provides important information for further elucidating the function and mechanisms of the CBL/CIPK network system responsing to stresses in foxtail millet.

Key words: Foxtail millet, Calcineurin B-like-interacting protein kinase, Stress

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