外源水杨酸、脯氨酸和γ-氨基丁酸对盐胁迫下水稻产量的影响

doi:10.3724/SP.J.1006.2017.01677

摘要/Abstract

摘要：

水杨酸(SA)、脯氨酸(Pro)和γ-氨基丁酸(GABA)在植物生长发育以及抵御逆境胁迫中起重要作用。为了研究三者复配对盐胁迫下水稻产量是否具有协同增效作用，本文采用三元二次通用旋转组合设计，建立了SA、Pro和GABA复配剂与盐胁迫下水稻产量之间的数学模型，通过频数分析获得SA、Pro与GABA复配优化组合方案。结果表明，二次模型拟合较好，对盐胁迫下水稻产量的影响程度为SA>GABA>Pro，SA与GABA，Pro与GABA之间交互作用对盐胁迫下水稻产量有显著影响。模拟寻优获得提高盐胁迫下水稻产量10%以上的最佳复配组合为SA0.44~0.51 mmol L^-1、Pro27.63~31.20 mmol L^-1、GABA3.55~4.28 mmol L^-1。外源SA、Pro与GABA单剂和复配剂对盐敏感品种牡丹江30的调控作用大于耐盐品种龙稻5。SA或Pro与GABA复配对盐胁迫下水稻产量的影响具有协同作用，而SA与Pro复配效果与品种耐盐性差异有关。SA、Pro和GABA三者复配效果优于两两复配，任意单剂与其他2种外源物质复配对水稻产量的影响均存在协同作用。

关键词: 二次通用旋转组合设计, 协同作用, 盐胁迫, 水稻, 产量

Abstract:

Salicylic acid (SA), proline (Pro) and γ-aminobutyric acid (GABA) play important roles in plant growth and development and tolerating stress. In order to investigate whether the combination of SA, Pro, and GABA had synergistic effect on the yield of rice under salt stress, the mathematical models were established by means of quadratic general rotary unitized design. The optimal combination of SA, Pro, and GABA was obtained by frequency analysis. The results demonstrated that the quadratic models fit better. The effects on the yield of rice under salt stress were SA>GABA>Pro. The interactions between SA and GABA, and between Pro and GABA showed significant effects on rice yield under salt stress.The recommended optimal combination is 0.44-0.51mmol L^-1for SA, 27.63-31.20mmol L^-1 for Pro and 3.55-4.28mmol L^-1for GABA, which can improve the rice yield more than 10% under salt stress. The effect of a single or a combinationof SA, Pro and GABA on the yield of salt-sensitive variety MDJ30 was larger than that of salt-tolerant variety LD5. The combination of SA and GABA, or Pro and GABA showed synergistic effect on the yield of rice under salt stress, while the combination effect of SA and Pro was related to the genotype difference in salt tolerance. The joint application of SA, Pro and GABA showed better effect on the yield of rice under salt stress than that of any two of them. The combination between any single agent and theother two agentsshowed synergistic effect.

Key words: Quadratic general rotary unitized design, Synergistic effects, Salt stress, Rice, Yield

沙汉景,胡文成,贾琰,王新鹏,田雪飞,于美芳,赵宏伟*. 外源水杨酸、脯氨酸和γ-氨基丁酸对盐胁迫下水稻产量的影响[J]. 作物学报, 2017, 43(11): 1677-1688.

SHA Han-Jing, HU Wen-Cheng, JIA Yan, WANG Xin-Peng, TIAN Xue-Fei, YU Mei-Fang, and ZHAO Hong-Wei*. Effect of Exogenous Salicylic Acid, Proline and γ-Aminobutyric Acid on Yield of Rice under Salt Stress[J]. Acta Agron Sin, 2017, 43(11): 1677-1688.

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