欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2018, Vol. 44 ›› Issue (12): 1837-1843.doi: 10.3724/SP.J.1006.2018.01837

• 耕作栽培·生理生化 • 上一篇    下一篇

增效缩节安化学封顶对棉花主茎生长的影响及其相关机制

安静1,黎芳1,周春江2,田晓莉1,*(),李召虎1   

  1. 1中国农业大学农学院 / 植物生长调节剂教育部工程研究中心, 北京 100193
    2北京市植物保护站, 北京 100029
  • 收稿日期:2018-05-06 接受日期:2018-08-20 出版日期:2018-12-12 网络出版日期:2018-09-19
  • 通讯作者: 田晓莉
  • 基金资助:
    本研究由国家现代农业产业技术体系建设专项(CARS-18-18);国家自然科学基金项目资助(31571588)

Morpho-physiological Responses of Cotton Shoot Apex to the Chemical Topping with Fortified Mepiquat Chloride

Jing AN1,Fang LI1,Chun-Jiang ZHOU2,Xiao-Li TIAN1,*(),Zhao-Hu LI1   

  1. 1 College of Agronomy and Biotechnology, China Agricultural University / Engineering Research Center of Plant Growth Regulator, Ministry of Education, Beijing 100193, China
    2 Beijing Plant Protection Station, Beijing 100029, China
  • Received:2018-05-06 Accepted:2018-08-20 Published:2018-12-12 Published online:2018-09-19
  • Contact: Xiao-Li TIAN
  • Supported by:
    This study was supported by the China Agricultural Research System(CARS-18-18);the National Natural Science Foundation of China(31571588)

RichHTML

8

PDF

612

摘要：

缩节安(1,1-dimethyl piperidinium chloride, DPC)是棉花生产中广泛应用的植物生长延缓剂。增效DPC (DPC +, 25%水剂)助剂中的成分能对植物幼嫩组织表面形成轻微伤害, 实践证明其可实现棉花化学封顶、起到替代人工打顶的作用。为探究DPC +作用机制, 本试验于2015年在田间条件下研究了棉花盛花期后(7月24日)应用DPC + (1125 mL hm -2)对棉花主茎生长和顶芽解剖结构、氧化还原状态及相关基因表达的影响。结果表明, 与对照(同期喷施清水)相比, DPC +处理后棉花株高降低, 白花以上节位(nodes above the last white flower, NAWF)更早降到5; 处理后3 d即可观察到主茎生长点较对照扁平, 生长点的纵横比显著低于对照; 处理后6 h棉花顶芽的O2 -、H2O2和MDA含量高于对照, 而开花相关基因GhSPL3和GhV1及顶端分生组织相关基因GhREV3的表达量则低于对照。化学封顶剂DPC +可引起棉株顶芽的短期氧化应激反应, 降低与主茎生长点发育和花芽分化相关基因的表达水平, 从而延缓棉株生长和花芽的产生, 实现化学封顶。

关键词: 棉花, 增效缩节安, 化学封顶, 顶芽解剖结构, 氧化还原, 基因表达

Abstract:

The plant growth regulator mepiquat chloride (1,1-dimethyl piperidinium chloride, DPC) has been successfully and worldwide used in cotton production. Fortified mepiquat chloride is a type of aqueous formulation containing 25% DPC (referred to DPC + hereafter), which can slightly damage young tissues of epidermis. DPC + has shown potential in cotton chemical topping in China, and may replace the conventional manual topping in future. In order to investigate the mechanism of cotton chemical topping with DPC +, this field study was conducted in 2015. DPC + (1125 mL ha -1) was applied after peak blooming stage on 24 July, with water as a control (CK). DPC + application significantly decreased plant height and reduced the nodes above the last white flower (NAWF) as compared with CK. After three days of DPC + treatment, cotton shoot apical meristem (SAM) became flatter than CK, and the ratio of height/length of SAM was significantly less than that of CK. With respect to redox status at shoot apex, O2 -production rate, H2O2 generation and MDA content were significantly increased at six hours after DPC + application. In addition, the expression of GhSPL3 (a SPL transcription factor, which might play an important role in bud differentiation, the transition of growth phase and flower formation), GhV1 (a B3-domain containing transcription factor, which potentially involved in floral initiation), and GhREV3 (a class III homeodomain-leucine zipper transcription factors, which has key roles in meristem and organ development) were down-regulated by DPC + also at six hours after application. In conclusion, DPC + application during later flowering period can implement cotton chemical topping by inducing short-time oxidative stress at cotton apex, down-regulating genes involved in SAM development, flower bud differentiation, and reducing cotton shoot growth.

Key words: cotton, fortified mepiquat chloride, chemical topping, anatomy of shoot apical meristem, redox status, gene expression

表1

qRT-PCR引物序列"

基因名称
Gene name		 引物序列
Primer Sequences (5'-3')		 基因序列来源
Gene source
GhSPL3 F: AAGGAGGGTCCAGTACGGTG; R: CAGCCTTGGCATGGAACTCA GenBank: KJ622311.1
GhV1 F: TGGGTGAGGGAGATGTCTGT; R: GGGCGGCATTTCATTGAGAC GenBank: GU929695
GhREV3 F: TATTCCGGCGCTGCGCTATG; R: TGTTGCTCAAGCTCTTGCCG Cottongen: CotAD_04603
GhACT9 F: GCCTTGGACTATGAGCAGGA; R: AAGAGATGGCTGGAAGAGGA GenBank: AY305737.1

图1

化学封顶剂DPC+对棉花株高的影响 比例尺: 10 cm。"

图2

化学封顶剂DPC+对棉花株高(A)及白花以上节位(B)的影响 误差线表示3次重复的标准误, *P < 0.05, ***P < 0.001。"

图3

化学封顶剂DPC+对棉花顶芽生长点解剖结构的影响 误差线表示3次重复的标准误, *P<0.05。比例尺: 100 μm。"

图4

化学封顶剂DPC+对棉花顶芽氧化还原状态的影响 误差线表示3次重复的标准误, *P < 0.05。"

[1] 中国农业科学院棉花研究所. 中国棉花栽培学. 上海: 上海科学技术出版社, 2013. pp 115-816
Cotton Research Institute, Chinese Academy of Agricultural Sciences. Cotton Farming in China. Shanghai: Shanghai Scientific and Technical Publishers, 2013. pp 115-816(in Chinese)
[2] Renou A, Téréta I, Togola M . Manual topping decreases bollworm infestations in cotton cultivation in Mali. Crop Prot, 2011,30:1370-1375
doi: 10.1016/j.cropro.2011.05.020
[3] Obasi M O, Msaakpa T S . Influence of topping, side branch pruning and hill spacing on growth and development of cotton(Gossypium barbadense L.) in the Southern Guinea Savanna location of Nigeria. J Agric Rural Dev Trop Subtrop, 2005,106:155-165
[4] Bennett O L, Ashley D A, Doss B D, Scarsbrook C E . Influence of topping and side pruning on cotton yield and other characteristics. Agron J, 1965,57:25-27
doi: 10.2134/agronj1965.00021962005700010009x
[5] 邹茜, 刘爱玉, 王欣悦, 向凤玲 . 棉花打顶技术的研究现状与展望. 作物研究, 2014,28:570-574
doi: 10.3969/j.issn.1001-5280.2014.05.27
Zou X, Liu A Y, Wang X Y, Xiang F L . Research progress and prospect on topping techniques in cotton. Crop Res, 2014,28:570-574 (in Chinese with English abstract)
doi: 10.3969/j.issn.1001-5280.2014.05.27
[6] 毛树春 . 我国棉花种植技术的现代化问题——兼论“十二五”棉花栽培相关研究. 中国棉花, 2010,37(3):2-6
doi: 1000-632X（2010）03-0002-04
Mao S C . On modernization of cotton planting in China-Related research on cotton cultivation from 2010 to 2015. China Cotton, 2010,37(3):2-6 (in Chinese)
doi: 1000-632X（2010）03-0002-04
[7] 牛巧鱼 . 我国棉花机械打顶研究进展. 中国棉花, 2013,40(11):23-24
doi: 10.3969/j.issn.1000-632X.2013.11.008
Niu Q Y . Research progress of cotton topping machinery in China. China Cotton, 2013,40(11):23-24 (in Chinese)
doi: 10.3969/j.issn.1000-632X.2013.11.008
[8] 李丕明, 何钟佩, 李召虎 . 棉花应用缩节安(DPC)化控技术研究概况与进展. 作物杂志, 1991, ( 2):1-3
Li P M, He Z P, Li Z H . Outline and progression about the study of DPC chemical control on cotton. Crops, 1991, ( 2):1-3 (in Chinese)
[9] 黎芳, 王希, 王香茹, 杜明伟, 周春江, 尹晓芳, 徐东永, 卢怀玉, 田晓莉, 李召虎 . 黄河流域北部棉区棉花缩节胺化学封顶技术. 中国农业科学, 2016,49:2497-2510
doi: 10.3864/j.issn.0578-1752.2016.13.005
Li F, Wang X, Wang X R, Du M W, Zhou C J, Yin X F, Xu D Y, Lu H Y, Tian X L, Li Z H . Cotton chemical topping with mepiquat chloride application in the north of Yellow River Valley of China. Sci Agric Sin, 2016,49:2497-2510 (in Chinese with English abstract)
doi: 10.3864/j.issn.0578-1752.2016.13.005
[10] 赵强, 周春江, 张巨松, 李松林, 恽友兰, 田晓莉 . 化学打顶对南疆棉花农艺和经济性状的影响. 棉花学报, 2011,23:329-333
doi: 10.3969/j.issn.1002-7807.2011.04.007
Zhao Q, Zhou C J, Zhang J S, Li S L, Yun Y L, Tian X L . Effect of chemical detopping on the canopy and yield of cotton (Gossypium hirsutum L.) in south Xinjiang. Cotton Sci, 2011,23:329-333 (in Chinese with English abstract)
doi: 10.3969/j.issn.1002-7807.2011.04.007
[11] 赵强, 张巨松, 周春江, 恽友兰, 李松林, 田晓莉 . 化学打顶对棉花群体容量的拓展效应. 棉花学报, 2011,23:401-407
doi: 10.3969/j.issn.1002-7807.2011.05.003
Zhao Q, Zhang J S, Zhou C J, Yun Y L, Li S L, Tian X L . Chemical detopping increases the optimum plant density in cotton (Gossypium hirsutum L.). Cotton Sci, 2011,23:401-407 (in Chinese with English abstract)
doi: 10.3969/j.issn.1002-7807.2011.05.003
[12] 潘明琪, 张建平, 齐文亮 . 化学打顶剂在棉花上的应用效果试验. 农村科技, 2011, ( 12):10-11
doi: 10.3969/j.issn.1002-6193.2011.12.008
Pan M Q, Zhang J P, Qi W L . Study the application effect of chemical topping agent on cotton. Rural Sci Tech, 2011, ( 12):10-11 (in Chinese)
doi: 10.3969/j.issn.1002-6193.2011.12.008
[13] 易正炳, 陈忠良, 刘海燕 . 化学打顶整枝剂在棉花上的应用效果研究. 中国农技推广, 2013,29(5):32-33
doi: 10.3969/j.issn.1002-381X.2013.05.016
Yi Z B, Chen Z L, Liu H Y . Study the application effect of chemical topping and pruning agent on cotton. China Agric Technol Extension, 2013,29(5):32-33 (in Chinese)
doi: 10.3969/j.issn.1002-381X.2013.05.016
[14] 吴葛, 刘向晖, 赵强, 易正炳 . 对玛纳斯县棉花化学打顶技术的调查与分析. 棉花科学, 2015,37(3):42-45
doi: 10.3969/j.issn.2095-3143.2015.03.010
Wu G, Liu X H, Zhao Q, Yi Z B . Investigation and analysis of the cotton topping in chemical technology in Manasi county. Cotton Sci, 2015,37(3):42-45 (in Chinese with English abstract)
doi: 10.3969/j.issn.2095-3143.2015.03.010
[15] Sergiev I, Alexieva V, Karanov E . Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Compt Rend Acad Bulg Sci, 1997,51:121-124
[16] 周祖富, 黎兆安 . 植物生理学实验指导. 北京: 中国农业出版社, 2005. pp 114-123
Zhou Z F, Li Z A. Plant Physiology Experiment Instruction. Beijing: China Agriculture Press, 2005. pp 114-123(in Chinese)
[17] Bondada B R, Oosterhuis D M . Canopy photosynthesis, specific leaf weight, and yield components of cotton under varying nitrogen supply. J Plant Nutr, 2001,24:469-477
doi: 10.1081/PLN-100104973
[18] Bourland F M, Benson N R, Vories E D, Tugwell N P, Danforth D M . Measuring maturity of cotton using nodes above white flower. J Cotton Sci, 2001,5:1-8
[19] 刁玉鹏, 谢方灵 . NAWF在美国棉花生产上的应用. 棉花学报, 1997,9:110-112
Diao Y P, Xie F L . Application NAWF in cotton production in USA. Cotton Sci, 1997,9:110-112 (in Chinese)
[20] 李正理 . 棉花形态学. 北京: 科学出版社, 1979. pp 55-69
Li Z L. Morphology of Cotton. Beijing: Science Press, 1979. pp 55-69(in Chinese)
[21] 李洁, 范术丽, 宋美珍, 庞朝友, 喻树迅 . 陆地棉GhSPL3基因的克隆、亚细胞定位及表达分析. 棉花学报, 2012,24:414-419
doi: 10.3969/j.issn.1002-7807.2012.05.006
Li J, Fan S L, Song M Z, Pang C Y, Yu S X . Cloning, subcellular localization and expression analysis ofGhSPL3 gene in Gossypium hirsutum L. Cotton Sci, 2012,24:414-419 (in Chinese with English abstract)
doi: 10.3969/j.issn.1002-7807.2012.05.006
[22] Wu M, Li J, Fan S L, Song M Z, Pang C Y, Wei J H, Yu J W, Zhang J F, Yu S X . Gene expression profiling in shoot apical meristem ofGossypium hirsutum. Russ J Plant Physiol, 2015,62:684-694
[23] Turchi L, Baima S, Morelli G, Ruberti I . Interplay of HD-Zip II and III transcription factors in auxin-regulated plant development. J Exp Bot, 2015,66:5043-5053
doi: 10.1093/jxb/erv174 pmid: 25911742
[24] Talbert P B, Adler H T, Parks D W, Comai L . The REVOLUTA gene is necessary for apical meristem development and for limiting cell divisions in the leaves and stems ofArabidopsis thaliana. Development, 1995,121:2723-2735
doi: 10.1101/gad.9.18.2314 pmid: 7555701
[25] Shi B H, Zhang C, Tian C H, Wang J, Wang Q, Xu T F, Xu Y, Ohno C, Sablowski R, Heisler M G, Theres K, Wang Y, Jiao Y L . Two-step regulation of a meristematic cell population acting in shoot branching in Arabidopsis. PLoS Genet, 2016,12:e1006168
doi: 10.1371/journal.pgen.1006168 pmid: 27398935
[26] 朱莹莹, 于亮亮, 汪杏芬, 李来庚 . HD-Zip III转录因子家族与植物细胞分化. 植物学报, 2013,48:199-209
Zhu Y Y, Yu L L, Wang X F, Li L G . HD-Zip III transcription factor and cell differentiation in plants. Chin Bull Bot, 2013,48:199-209 (in Chinese with English abstract)
[27] Gill S S, Tuteja N . Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem, 2010,48:909-930
doi: 10.1016/j.plaphy.2010.08.016 pmid: 20870416
[28] 王宁, 田晓莉, 段留生, 严根土, 黄群, 李召虎 . 缩节胺浸种提高棉花幼苗根系活力中的活性氧代谢. 作物学报, 2014,40:1220-1226
Wang N, Tian X L, Duan L S, Yan G T, Huang Q, Li Z H . Metabolism of reactive oxygen species involved in increasing root vigour of cotton seedlings by soaking seeds with mepiquat chloride. Acta Agron Sin, 2014,40:1220-1226 (in Chinese with English abstract)
[29] 薛鑫, 张芊, 吴金霞 . 植物体内活性氧的研究及其在植物抗逆方面的应用. 生物技术通报, 2013, ( 10):6-11
Xue X, Zhang Q, Wu J X . Research of reactive oxygen species in plants and its application on stress tolerance. Biotechnol Bull, 2013, ( 10):6-11 (in Chinese with English abstract)
[30] Zhang X H, Dou L L, Pang C Y, Song M Z, Wei H L, Fan S L, Wang C S, Yu S X . Genomic organization, differential expression, and functional analysis of the SPL gene family in Gossypium hirsutum. Mol Genet Genomics, 2015,290:115-126
doi: 10.1007/s00438-014-0901-x pmid: 25159110
[31] Qiu C X, Zuo K J, Qin J, Zhao J Y, Ling H, Tang K X . Isolation and characterization of a class III homeodomain-leucine zipper-like gene from Gossypium barbadense. DNA Seq, 2006,17:334-341
doi: 10.1080/10425170600824335 pmid: 17343206
[32] 娄善伟, 赵强, 朱北京, 魏欢 . 棉花化学封顶对植株上部枝叶形态变化的影响. 西北农业学报, 2015,24(8):62-67
doi: 10.7606/j.issn.1004-1389.2015.08.011
Lou S W, Zhao Q, Zhu B J, Wei H . Effect of chemical topping on morphologic changes of leaves and branches in upper part of cotton. Acta Agric Boreali-Occident Sin, 2015,24(8):62-67 (in Chinese with English abstract)
doi: 10.7606/j.issn.1004-1389.2015.08.011
[33] 杨成勋, 张旺锋, 徐守振, 随龙龙, 梁福斌, 董恒义 . 喷施化学打顶剂对棉花冠层结构及群体光合生产的影响. 中国农业科学, 2016,49:1672-1684
doi: 10.3864/j.issn.0578-1752.2016.09.004
Yang C X, Zhang W F, Xu S Z, Sui L L, Liang F B, Dong H Y . Effects of spraying chemical topping agents on canopy structure and canopy photosynthetic production in cotton. Sci Agric Sin, 2016,49:1672-1684 (in Chinese with English abstract)
doi: 10.3864/j.issn.0578-1752.2016.09.004
[1] 李海芬, 魏浩, 温世杰, 鲁清, 刘浩, 李少雄, 洪彦彬, 陈小平, 梁炫强. 花生电压依赖性阴离子通道基因(AhVDAC)的克隆及在果针向地性反应中表达分析[J]. 作物学报, 2022, 48(6): 1558-1565.
[2] 周静远, 孔祥强, 张艳军, 李雪源, 张冬梅, 董合忠. 基于种子萌发出苗过程中弯钩建成和下胚轴生长的棉花出苗壮苗机制与技术[J]. 作物学报, 2022, 48(5): 1051-1058.
[3] 孙思敏, 韩贝, 陈林, 孙伟男, 张献龙, 杨细燕. 棉花苗期根系分型及根系性状的关联分析[J]. 作物学报, 2022, 48(5): 1081-1090.
[4] 姚晓华, 王越, 姚有华, 安立昆, 王燕, 吴昆仑. 青稞新基因HvMEL1 AGO的克隆和条纹病胁迫下的表达[J]. 作物学报, 2022, 48(5): 1181-1190.
[5] 闫晓宇, 郭文君, 秦都林, 王双磊, 聂军军, 赵娜, 祁杰, 宋宪亮, 毛丽丽, 孙学振. 滨海盐碱地棉花秸秆还田和深松对棉花干物质积累、养分吸收及产量的影响[J]. 作物学报, 2022, 48(5): 1235-1247.
[6] 郑曙峰, 刘小玲, 王维, 徐道青, 阚画春, 陈敏, 李淑英. 论两熟制棉花绿色化轻简化机械化栽培[J]. 作物学报, 2022, 48(3): 541-552.
[7] 渠建洲, 冯文豪, 张兴华, 徐淑兔, 薛吉全. 基于全基因组关联分析解析玉米籽粒大小的遗传结构[J]. 作物学报, 2022, 48(2): 304-319.
[8] 张艳波, 王袁, 冯甘雨, 段慧蓉, 刘海英. 棉籽油分和3种主要脂肪酸含量QTL分析[J]. 作物学报, 2022, 48(2): 380-395.
[9] 张特, 王蜜蜂, 赵强. 滴施缩节胺与氮肥对棉花生长发育及产量的影响[J]. 作物学报, 2022, 48(2): 396-409.
[10] 陈新宜, 宋宇航, 张孟寒, 李小艳, 李华, 汪月霞, 齐学礼. 干旱对不同品种小麦幼苗的生理生化胁迫以及外源5-氨基乙酰丙酸的缓解作用[J]. 作物学报, 2022, 48(2): 478-487.
[11] 赵文青, 徐文正, 杨锍琰, 刘玉, 周治国, 王友华. 棉花叶片响应高温的差异与夜间淀粉降解密切相关[J]. 作物学报, 2021, 47(9): 1680-1689.
[12] 岳丹丹, 韩贝, Abid Ullah, 张献龙, 杨细燕. 干旱条件下棉花根际真菌多样性分析[J]. 作物学报, 2021, 47(9): 1806-1815.
[13] 王艳朋, 凌磊, 张文睿, 王丹, 郭长虹. 小麦B-box基因家族全基因组鉴定与表达分析[J]. 作物学报, 2021, 47(8): 1437-1449.
[14] 曾紫君, 曾钰, 闫磊, 程锦, 姜存仓. 低硼及高硼胁迫对棉花幼苗生长与脯氨酸代谢的影响[J]. 作物学报, 2021, 47(8): 1616-1623.
[15] 宋天晓, 刘意, 饶莉萍, Soviguidi Deka Reine Judesse, 朱国鹏, 杨新笋. 甘薯细胞壁蔗糖转化酶基因IbCWIN家族成员鉴定及表达分析[J]. 作物学报, 2021, 47(7): 1297-1308.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2