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作物学报 ›› 2019, Vol. 45 ›› Issue (5): 647-655.doi: 10.3724/SP.J.1006.2019.84123
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
李乐晨1,朱国忠1,苏秀娟1,2,郭旺珍1,*(
)
Le-Chen LI1,Guo-Zhong ZHU1,Xiu-Juan SU1,2,Wang-Zhen GUO1,*()
摘要：
海岛棉具有纤维品质好、抗病性强等优异性状, 不仅为纺织工业提供优质棉纤维原料, 也是陆地棉相关性状改良的重要供体材料。然而, 与陆地棉相比, 开展海岛棉的遗传多样性和基因分型研究相对较少。本研究基于CottonSNP80K芯片对282份不同来源的海岛棉品种/材料进行基因组SNP分型研究, 以选择高效鉴别海岛棉材料的核心位点组合。按照检出率大于95%、具多态性、最小等位频率(MAF)大于0.01、杂合率小于0.05、无冗余位点等条件筛选, 获得2594个高质量SNP位点。对上述位点设置不同数目梯度筛选, 确定最优核心位点数。随着位点个数的增多, 位点组合对海岛棉材料的识别率逐渐增加。当位点数为200时, 识别率为89%; 位点数提高到1500时, 识别率可达99%。进一步增加位点数, 识别率无显著变化。利用中选的1500个SNP位点检测供试材料, 其平均MAF值0.14, 平均杂合率0.007, 平均多态信息含量0.21。SNP位点的聚丙烯凝胶电泳验证表明, SNP-PCR与芯片分型结果一致性达98.3%。本研究提供了适于海岛棉指纹图谱构建、含1500位点的一套核心SNP位点组合, 可用于海岛棉遗传多样性分析和品种身份鉴定。
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