欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2020, Vol. 46 ›› Issue (02): 269-279.doi: 10.3724/SP.J.1006.2020.93015

• 耕作栽培·生理生化 • 上一篇    下一篇

不同成膜剂对玉米噻虫啉悬浮种衣剂的持效性及安全性影响

姚晨涛1,2,4,乔治华1,2,宋雪慧1,2,张风文1,2,孙晓1,2,李刚1,李向东1,张吉旺3,姜兴印1,2,*()   

  1. 1 山东农业大学植物保护学院, 山东泰安271018
    2 山东农业大学植物保护学院农药毒理与应用技术省级重点实验室, 山东泰安271018
    3 山东农业大学作物国家重点实验室, 山东泰安271018
    4 苏州富美实植物保护剂有限公司, 上海 201203
  • 收稿日期:2019-03-08 接受日期:2019-09-26 出版日期:2020-02-12 网络出版日期:2019-10-12
  • 通讯作者: 姜兴印
  • 作者简介:E-mail: yaoct2009@163.com
  • 基金资助:
    本研究由国家重点研发计划重点项目(2018YFD0200604);山东“双一流”奖补项目(农作物病虫害绿色防控)(SYL2017XTTD11);山东省现代农业产业技术体系项目资助(SDAIT-02-10)

Effect of different film-forming agents on the persistence and safety of thiacloprid suspension concentrate for seed coating for maize

YAO Chen-Tao1,2,4,QIAO Zhi-Hua1,2,SONG Xue-Hui1,2,ZHANG Feng-Wen1,2,SUN Xiao1,2,LI Gang1,LI Xiang-Dong1,ZHANG Ji-Wang3,JIANG Xing-Yin1,2,*()   

  1. 1 College of Plant Protection, Shandong Agricultural University, Tai’an 271018, Shandong, China
    2 College of Plant Protection, Shandong Agricultural University, Key Laboratory of Pesticide Toxicology & Application Technique, Tai’an 271018, Shandong, China
    3 Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai’an 271018, Shandong, China
    4 Suzhou FMC Plant Protection Co., Ltd., Shanghai 201203, China
  • Received:2019-03-08 Accepted:2019-09-26 Published:2020-02-12 Published online:2019-10-12
  • Contact: Xing-Yin JIANG
  • Supported by:
    This study was supported by the National Key Research and Development Project(2018YFD0200604);the Provincial Major Technological Innovation Program of Agricultural Application in Shandong, the Shandong “Double First-class” Award(SYL2017XTTD11);the National Modern Agricultural Technology & Industry System(SDAIT-02-10)

RichHTML

4

PDF

198

摘要：

为明确含不同成膜剂的噻虫啉种衣剂处理玉米种子后在土壤和植株中的持效性及其对刺吸式害虫的防治效果, 选择3种不同成膜剂加工成的噻虫啉悬浮种衣剂以400 g a.i. 100 kg -1种子浓度包衣处理, 分别于室内和田间条件下测定其对玉米出苗率、生长量和生理生化等方面的影响, 及噻虫啉在玉米叶片和土壤中的持效期, 并评价其对刺吸式害虫的防治效果。结果表明, ZY904-3和ZY904-5成膜剂的噻虫啉种衣剂处理对玉米出苗没有影响, 3种成膜剂的噻虫啉拌种对玉米生长量和生理生化活性均有不同程度促进, 玉米叶片和土壤中的噻虫啉持效期较未加成膜剂有一定延长, ZY904-1、ZY904-3和ZY904-5处理的噻虫啉悬浮种衣剂(FS)在叶片和土壤中持效期分别延长了20~40 d、20~30 d和10~20 d。3种成膜剂处理可有效防治玉米刺吸式害虫, ZY904-1和ZY904-3成膜剂的噻虫啉种衣剂处理对玉米刺吸式害虫数量有很好的控制作用, 分别于播种80 d (室内)和60 d (田间)后防效仍达50%以上, 但ZY904-1成膜剂的噻虫啉种衣剂推迟出苗时间。综合各个方面, ZY904-3成膜剂的噻虫啉种衣剂拌种效果最好。

关键词: 噻虫啉, 玉米, 不同成膜剂, 持效期, 安全性, HPLC-MS/MS

Abstract:

To definite the persistence of thiacloprid seed coating with different film-forming agents in soil and plants and the efficacy in controling piercing-sucking insects, we tested the effects of the thiacloprid FS, with three different film-forming agents at a concentration of 400 g a.i. 100 kg -1, on the emergence rate, growth, physiological and biochemical characteristics of maize at laboratory and in the field respectively, and evaluated controlling effect on piercing-sucking insects by high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). The ZY904-3 and ZY904-5 film-forming agent treatments had no influence on emergence rate of maize. The seedling growth and activities in physiology and biochemistry were promoted to various degrees by thiacloprid seed dressing with three different film-forming agents. Compared with no film-forming agent treatments, the persistent periods of ZY904-1, ZY904-3, and ZY904-5 treatments were extended by 20-40 days, 20-30 days, and 10-20 days in leaves of maize and in the soil respectively. The maize piercing-sucking insects were controlled significantly by three film-forming agent treatments. ZY904-1 and ZY904-3 treatments had good control effects on the amount of piercing-sucking insects in the growth stage of maize which coat still over 50% at 80 d (laboratory test) and 60 d (field test) after sowing, respectively. However, ZY904-1 treatment delayed the emergence of seeds. Taking all factors as a whole, ZY904-3 film-forming agent has the best seed dressing effect.

Key words: thiacloprid, maize, film-forming agent, persistent period, safety, liquid chromatography-mass

表1

梯度洗脱程序"

时间
Time
(min)		 流速
Flow rate
(mL min-1)		 流动相 Mobile phase (%)
0.1%甲酸水溶液
0.1% formic acid
solution		 乙腈
Acetonitrile
0 0.50 90 10
1 0.50 90 10
2 0.50 10 90
3 0.50 10 90
4 0.50 90 10
5 0.50 90 10

表2

不同成膜剂噻虫啉种衣剂对玉米出苗率的影响"

成膜剂
Film-forming agent		 有效成分
Active ingredients
(g a.i. 100 kg-1)		 室内试验
Laboratory
test		 大田试验Field test
2 d 4 d 6 d 8 d 10 d
ZY904-1 400 80.27±1.35 b 56.26±1.65 b 76.81±2.67 c 78.56±2.30 c 84.31±1.74 b 84.63±1.32 b
ZY904-3 400 96.58±2.26 a 63.17±2.64 a 85.60±1.62 a 92.61±0.98 a 93.48±3.19 a 93.48±1.19 a
ZY904-5 400 95.42±1.74 a 63.40±1.37 a 85.35±2.09 a 91.49±1.35 a 92.24±2.34 a 92.24±2.34 a
未添加成膜剂对照NCK 400 96.33±2.88 a 64.24±0.96 a 86.77±1.79 a 91.70±3.36 a 93.38±3.18 a 93.38±3.18 a
清水对照CK — 97.31±2.57 a 58.63±4.74 b 82.91±1.42 b 86.56±2.34 b 90.77±1.54 ab 90.77±4.54 ab

表3

不同成膜剂噻虫啉拌种对玉米幼苗生长指标的影响(室内试验)"

成膜剂
Film-forming agent		 有效成分
Active ingredients
(g a.i. 100 kg-1)		 株高
Plant height
(cm)		 根长
Root length
(cm)		 茎粗
Stem thick
(cm)		 须根数
Fibrous root number		 鲜重
Fresh weight
(g)
ZY904-1 400 30.60±2.31 b 18.62±2.46 a 6.18±0.53 a 5.2±0.32 a 4.91±0.28 a
ZY904-3 400 32.35±1.34 a 19.85±1.28 a 6.42±0.47 a 5.7±0.62 a 5.07±0.46 a
ZY904-5 400 32.26±1.86 a 18.23±3.25 a 6.23±0.38 a 5.3±0.35 a 4.73±0.19 ab
未添加成膜剂对照NCK 400 33.21±1.81 a 18.64±2.45 a 6.55±0.41 a 5.2±0.28 a 4.63±0.24 ab
清水对照CK — 33.47±1.69 a 18.98±3.12 a 6.83±0.48 a 5.1±0.19 a 4.25±0.64 b

表4

不同成膜剂噻虫啉拌种对玉米幼苗生长指标的影响(大田试验)"

成膜剂
Film-forming agent		 有效成分
Active ingredients
(g a.i. 100 kg-1)		 株高
Plant height
(cm)		 根长
Root length
(cm)		 茎粗
Stem thick
(cm)		 须根数
Fibrous root number		 鲜重
Fresh weight
(g)
ZY904-1 400 29.64±1.32 a 11.65±0.56 a 7.80±0.35 a 6.1±0.02 a 4.76±0.33 a
ZY904-3 400 27.32±0.82 a 11.85±0.36 a 8.61±0.56 a 5.8±0.06 a 4.83±0.54 a
ZY904-5 400 29.47±0.66 a 11.42±1.10 a 9.00±0.44 a 6.0±0.00 a 5.04±0.28 a
未添加成膜剂对照NCK 400 30.36±1.98 a 12.56±0.45 a 8.55±0.32 a 6.0±0.03 a 5.06±0.42 a
清水对照CK — 23.24±1.33 b 8.71±1.32 b 5.83±0.24 a 5.2±0.05 a 3.77±0.74 b

表5

不同成膜剂噻虫啉拌种对玉米常规生理指标的影响"

成膜剂
Film-forming agent		 有效成分
Active
ingredient
(g a.i. 100 kg-1)		 叶绿素
Chlorophyll
(mg g-1 FW)		 根系活力
Root activity
(g h-1 FW)		 游离脯氨酸
Free proline accumulation
(μg g-1 DW)
室内试验
Laboratory		 田间试验
Field		 室内试验
Laboratory		 田间试验
Field		 室内试验
Laboratory		 田间试验
Field
ZY904-1 400 2.21±0.11 a 2.34±0.03 a 0.35±0.04 a 0.54±0.01 a 57.24±0.94 b 87.37±1.21 a
ZY904-3 400 2.16±0.27 a 2.33±0.02 a 0.38±0.08 a 0.55±0.03 a 62.39±1.32 a 87.11±1.29 a
ZY904-5 400 2.19±0.09 a 2.34±0.03 a 0.37±0.08 a 0.52±0.02 ab 59.22±0.72 b 86.82±0.65 a
未添加成膜剂对照NCK 400 2.04±0.07 ab 2.23±0.08 a 0.32±0.02 a 0.50±0.06 a 57.49±1.32 b 80.46±0.95 a
清水对照CK — 1.94±0.11 b 1.85±0.07 b 0.24±0.02 b 0.46±0.03 b 42.16±0.64 c 54.90±1.47 b

表6

不同成膜剂噻虫啉拌种对玉米抗氧化酶活性的影响"

成膜剂
Film-forming agent		 有效成分
Active ingredient
(g a.i. 100 kg-1)		 超氧化物歧化酶活性
SOD activity
(U g-1 FW min-1)		 过氧化氢酶活性
CAT activity
(U g-1 FW min-1)		 过氧化物酶活性
POD activity
(U g-1 FW min-1)
室内试验
Laboratory		 田间试验
Field		 室内试验
Laboratory		 田间试验
Field		 室内试验
Laboratory		 田间试验
Field
ZY904-1 400 71.36±2.32 a 50.85±0.96 a 134.23±4.28 a 81.74±2.47 a 64.82±4.53 a 55.30±2.12 a
ZY904-3 400 76.53±3.46 a 51.62±1.30 a 147.65±6.42 a 79.84±0.30 a 60.17±5.26 a 55.06±3.45 a
ZY904-5 400 80.32±2.49 a 50.06±0.84 a 130.27±8.24 a 80.70±2.44 a 64.22±2.79 a 54.23±1.41 a
未添加成膜剂对照NCK 400 74.21±4.12 a 46.32±1.31 a 140.59±7.33 a 74.33±2.44 a 62.47±4.62 a 45.29±2.63 ab
清水对照CK — 58.34±1.91 b 35.64±1.09 b 80.69±9.28 b 64.64±1.62 b 47.82±4.33b 36.53±2.34 c

表7

噻虫啉在玉米叶片和土壤中的添加回收率"

添加水平
Spiked level
(mg kg-1)		 标准曲线
Calibration curve		 土壤Soil 玉米植株Maize plant
平均回收率
Average recovery (%)		 相对标准偏差
RSD (%)		 平均回收率
Average recovery (%)		 相对标准偏差
RSD (%)
0.01 y = 3775.92x + 6978240
R2 = 0.9931		 89.42 6.4 86.07 7.9
0.1 94.64 3.4 95.36 2.9
1.0 96.78 3.2 95.36 5.6

图1

噻虫啉标样质谱图"

图2

不同成膜剂噻虫啉拌种处理在玉米叶片中的消解动态"

图3

不同成膜剂噻虫啉拌种处理在土壤中的残留动态"

图4

不同成膜剂噻虫啉拌种对刺吸式害虫数量的影响"

[1] 张学林, 徐钧, 安婷婷, 侯小畔, 李潮海 . 不同氮肥水平下玉米根际土壤特性与产量的关系. 中国农业科学, 2016,49:2687-2699.
doi: 10.3864/j.issn.0578-1752.2016.14.004
Zhang X L, Xu J, An T T, Hou X P, Li H C . Relationship between rhizosphere soil properties and yield of maize at different nitrogen levels. Sci Agric Sin, 2016,49:2687-2699 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2016.14.004
[2] 刘微, 张晓翔, 彭辉, 王彪, 王永生, 仪美芹, 王晶杰, 杜鹏, 宋震洋 . 不同药剂配方种子包衣对玉米出苗安全性及玉米丝黑穗病和蚜虫的防治效果. 植物保护, 2017,43:224-229.
Liu W, Zhang X X, Peng H, Wang B, Wang Y S, Yi M Q, Wang J J, Du P, Song Z Y . Safety of different seed coating formulations to maize seeding and the control effect against maize head smut and aphids. Plant Prot, 2017,43:224-229 (in Chinese with English abstract).
[3] 王坤 . 马铃薯、玉米邻作对大豆田刺吸式害虫的生态调控. 东北农业大学硕士学位论文, 黑龙江哈尔滨, 2017.
Wang K . Potato and Corn Neighbors Regulate Insect Pests in Soybean Fields. MS Thesis of Northeast Agricultural University, Harbin, Heilongjiang, China, 2017 (in Chinese with English abstract).
[4] Asin L, Pons X . Effect of high temperature on the growth and reproduction of corn aphids (Homoptera: Aphididae) and implications for their population dynamics on the northeastern Iberian peninsula. Environ Entomol, 2001,30:1127-1134.
doi: 10.1603/0046-225X-30.6.1127
[5] Manandhar R, Wright M G . Effects of interplanting flowering plants on the biological control of corn earworm (Lepidoptera: Noctuidae) and thrips (Thysanoptera: Thripidae) in sweet corn. J Econ Entomol, 2016,109:113-119.
doi: 10.1093/jee/tov306 pmid: 26500338
[6] 杨菲, 张爱红, 闫冲, 孟凡思, 霍良占, 邸垫平, 苗洪芹, 王锡锋 . 人工饲养不同世代灰飞虱群体传播水稻黑条矮缩病毒的比较. 植物病理学报, 2017,47:551-557.
Yang F, Zhang A H, Yan C, Meng F S, Huo Z L, Di D P, Miao H Q, Wang T F . Comparison of RBSDV bearing rates and transmission rates among different generations of artificial reared Laodelphax striatellus Fallén. Acta Phytopathol Sin, 2017,47:551-557 (in Chinese with English abstract).
[7] 田体伟, 王丽莎, 王燕, 郭线茹, 闫凤鸣, 李洪连 . 3种新烟碱类种子处理剂对玉米及其主要害虫的影响. 河南农业科学, 2015,44(11):73-78.
Tian T W, Wang L S, Wang Y, Guo X R, Yan F M, Li H L . Effects of seed coating with imidacloprid and thiamethoxam on maize and main pests. J Henan Agric Sci, 2015,44(11):73-78 (in Chinese with English abstract).
[8] 高志山, 张学峰, 刘海涛, 张文娟, 慕卫 . 新烟碱类杀虫剂种子包衣防治麦蚜的可行性评价. 植物保护学报, 2016,43:864-872.
Gao Z S, Zhang X F, Liu H T, Zhang W J, Mu W . Feasibility evaluation of neonicotinoid insecticide seed coating for the control of wheat aphid. Plant Prot, 2016,43:864-872 (in Chinese with English abstract).
[9] 卜成成, 姜兴印, 芦勇, 李刚, 邢则森, 张吉旺, 王振林 . 噻虫啉拌种对小麦种子及幼苗生长的生理效应. 中国农学通报, 2017,33(21):1-6.
Bu C C, Jiang X Y, Lu Y, Li G, Xing Z S, Zhang J W, Wang Z L . Thiacloprid seed dressing: the physiological effect on seed and seedling growth of wheat. Chin Agric Sci Bull, 2017,33(21):1-6 (in Chinese with English abstract).
[10] Brandt A, Gorenflo A, Siede R, Meixner M, Büchler R . The neonicotinoids thiacloprid, imidacloprid, and clothianidin affect the immunocompetence of honey bees (Apis mellifera L.). J Insect Physiol, 2016,86:40-47.
doi: 10.1016/j.jinsphys.2016.01.001 pmid: 26776096
[11] Poletti M, Maia A, Omoto C . Toxicity of neonicotinoid insecticides to Neoseiulus californicus and Phytoseiulus macropilis (Acari: Phytoseiidae) and their impact on functional response to Tetranychus urticae (Acari: Tetranychidae). Biol Control, 2007,40:30-36.
doi: 10.1016/j.biocontrol.2006.09.001
[12] 季守民, 程传英, 袁传卫, 周秀玲, 姜兴印 . 7种新烟碱类杀虫剂对意大利蜜蜂的急性毒性及风险评价. 农药, 2015,54(4):282-285.
Ji S M, Cheng C Y, Yuan C W, Zhou X L, Jiang X Y . Acute toxicity and risk assessment of seven neonicotinoid insecticides on honeybees (Apis mellifera L.). Agrochemicals, 2015,54(4):282-285 (in Chinese with English abstract).
[13] 蔺哲广, 孟飞, 郑火青, 周婷, 胡福良 . 新烟碱类杀虫剂对蜜蜂健康的影响. 昆虫学报, 2014,57:607-615.
Lin Z G, Meng F, Zheng H Q, Zhou T, Hu F L . Effects of neonicotinoid insecticides on honeybee health. Acta Entomol Sin, 2014,57:607-615 (in Chinese with English abstract).
[14] 齐麟, 王昱翔, 王宁, 段一鸣, 张盈, 王娅, 肖璐璐, 李晓刚 . 40%噻虫嗪·吡唑醚菌酯悬浮种衣剂成膜及生物学特性. 中国农业科学, 2017,50:1624-1634.
doi: 10.3864/j.issn.0578-1752.2017.09.008
Qi L, Wang Y X, Wang N, Duan Y M, Zhang Y, Wang Y, Xiao L L, Li X G . Film formation and biological properties of 40% thiamethoxam•pyraclostrobin flowable concentrate for seed coating. Sci Agric Sin, 2017,50:1624-1634 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2017.09.008
[15] Zeng D, Shi Y . Preparation and application of a novel environmentally friendly organic seed coating for rice. J Sci Food Agric, 2010,89:2181-2185.
doi: 10.1002/jsfa.v89:13
[16] 邓理楠, 李保同, 徐月明, 石庆华, 潘晓华 . 2种氟虫双酰胺复配制剂拌种对直播晚稻蓟马的控制效果及水稻生长的影响. 中国农学通报, 2011,27(12):286-290.
Deng L N, Li B T, Xu M Y, Shi Q H, Pan X H . Effects of mixed preparation of two kinds of fluorophora diamide on control effect and growth of rice in late rice thistle. Chin Agric Sci Bull, 2011,27(12):286-290 (in Chinese with English abstract).
[17] Liu Z, Dai Y, Huang G, Gu Y, Ni J, Wei H, Yuan S . Soil microbial degradation of neonicotinoid insecticides imidacloprid, acetamiprid, thiacloprid and imidaclothiz and its effect on the persistence of bioefficacy against horsebean aphidAphis craccivora Koch after soil application. Pest Manage Sci, 2011,67:1245-1252.
doi: 10.1002/ps.2174 pmid: 21538797
[18] 齐麟, 王昱翔, 王宁, 段一鸣, 张盈, 肖璐璐, 王娅, 李晓刚 . 水稻种衣剂成膜助剂的研究进展. 种子, 2017,36(6):54-60.
Qi L, Wang Y X, Wang N, Duan Y M, Zhang Y, Wang Y, Xiao L L, Li X G . Research progress of film forming assistant of rice seed coat agent. Seed, 2017,36(6):54-60 (in Chinese with English abstract).
[19] 董永义, 韩宁宁 . 种衣剂对玉米出苗和幼苗生长的影响. 江苏农业科学, 2016,44(4):149-151.
Dong Y Y, Han N N . Effects of seed dressing on seedling growth and seedling growth of maize. Jiangsu Agric Sci, 2016,44(4):149-151 (in Chinese with English abstract).
[20] 张梦晗, 杨换玲, 郭线茹, 雷彩燕, 闫凤鸣 . 吡虫啉种衣剂对小麦种子萌发和幼苗生长的影响及相关生理机制. 河南农业科学, 2015,44(8):76-79.
Zhang M H, Yang H L, Guo X R, Lei C Y, Yan F M . Effects of imidacloprid seed coating agent on wheat seeds germination and seedlings growth and related physiological mechanism. J Henan Agric Sci, 2015,44(8):76-79 (in Chinese with English abstract).
[21] Huang G B, Chai Q, Feng F X, Yu A Z . Effects of different tillage systems on soil properties, root growth, grain yield, and water use efficiency of winter wheat (Triticum aestivum L.) in arid northwest China. J Integr Agric, 2012,11:1286-1296.
doi: 10.1016/S2095-3119(12)60125-7
[22] 程传英, 袁传卫, 殷万元, 张风文, 姜兴印 . 3种甲氧基丙烯酸酯类杀菌剂拌种对花生幼苗生长及生理作用的影响. 植物生理学报, 2015,51:337-344.
Cheng C Y, Yuan C W, Yin W Y, Zhang F W, Jiang X Y . Effects of seed dressing with three kinds of strobilurin fungicides on the growth and physiological function of peanut (Arachis hypogaea) seedling. Plant Physiol J, 2015,51:337-344 (in Chinese with English abstract).
[23] Anjum S, Farooq M, Wang L C, Xue L, Wang S G, Wang L C, Zhang S . Gas exchange and chlorophyll synthesis of maize cultivars are enhanced by exogenously-applied glycinebetaine under drought conditions. Plant Soil Environ, 2011,57:326-331.
[24] Jiang M, Zhang J . Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. J Exp Bot, 2002,53:2401-2410.
doi: 10.1093/jxb/erf090 pmid: 12432032
[25] Suzuki N, Katano K . Coordination between ROS regulatory systems and other pathways under heat stress and pathogen attack. Front Plant Sci, 2018,9:490.
doi: 10.3389/fpls.2018.00490 pmid: 29713332
[26] Westhuizen A J V D, Qian X M, Botha A M . Differential induction of apoplastic peroxidase and chitinase activities in susceptible and resistant wheat cultivars by Russian wheat aphid infestation. Plant Cell Rep, 1998,18:132-137.
doi: 10.1007/s002990050545
[27] 袁传卫 . 吡唑醚菌酯拌种对玉米生理特性及土壤微生物的影响. 山东农业大学硕士学位论文, 山东泰安, 2015.
Yuan C W . The Effect of Seed Coating of Pyraclostrobin on Physiological Characteristics of Maize and Soil Microbial. MS Thesis of Shandong Agricultural University, Tai’an, Shandong, China, 2015 (in Chinese with English abstract).
[28] 李金玉, 刘桂英 . 良种包衣新产品: 药肥复合型种衣剂. 种子, 1990, ( 6):53-56.
Li J Y, Liu G Y . New product of fine seed coat: compound seed coat of medicine and fertilizer. Seed, 1990, ( 6):53-56 (in Chinese with English abstract).
[29] 董飒, 张秀焕, 王杰, 夏晓明, 王开运 . 36%噻虫啉水分散粒剂对番茄幼苗根系活力及生理生化指标的影响. 农药学学报, 2014,16(1):35-40.
Dong S, Zhang X H, Wang J, Xia X M, Wang K Y . Effects of thiacloprid 360WG on physiological indices and root vigor in tomato seedling. Chin J Pestic Sci, 2014,16(1):35-40 (in Chinese with English abstract).
[30] 董国政 . 典型农药对设施菜地土壤质量与番茄生长的影响. 中国农业科学院硕士学位论文, 北京, 2012.
Dong G Z . Effects of Typical Pesticides on Soil Quality and Tomato Growth in Vegetable Fields. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing, China, 2012 (in Chinese with English abstract).
[1] 刘恩波, 陈静, 李红星, 于宁宁, 任佰朝, 赵斌, 刘鹏, 张吉旺. 遮阴改变源-库平衡和调节碳水化合物代谢进而抑制夏玉米幼穗发育[J]. 作物学报, 2026, 52(6): 1891-1901.
[2] 梁进宇, 尹嘉德, 王红丽, 张国平, 侯慧芝, 董博, 马明生. 基于无人机高光谱和机器学习的旱地饲用玉米叶片氮含量估测[J]. 作物学报, 2026, 52(6): 1788-1801.
[3] 孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308.
[4] 张宁宁, 滕雨菲, 任娜娜, 魏兴卓, 闫书豪, 樊可心, 王永宏, 陈文康, 张兴华, 朱万超, 徐淑兔, 薛吉全. 201份玉米自交系抗旱表型评价及可塑性分析[J]. 作物学报, 2026, 52(5): 1309-1325.
[5] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[6] 张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500.
[7] 杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590.
[8] 韩亚鑫, 何冠华, 张小琼, 张登峰, 李永祥, 刘旭洋, 王天宇, 黎裕, 邹华文, 李春辉. 基于RNA-Seq和BSA-Seq联合分析挖掘玉米侧根密度基因资源[J]. 作物学报, 2026, 52(5): 1341-1352.
[9] 蔡宏玮, 于爱忠, 姜科强, 王鹏飞, 王玉珑, 霍建喆, 庞小能, 尹波, 尚永盼. 干旱灌区有机肥替代部分化肥促进甜玉米产量提升的关键机制[J]. 作物学报, 2026, 52(4): 1166-1180.
[10] 田红丽, 杨扬, 范亚明, 易红梅, 郭丹丹, 王凤格, 赵久然. 适于玉米品种鉴定的一套三等位变异SNP新型标记组合[J]. 作物学报, 2026, 52(4): 993-1005.
[11] 杨亚莉, 徐明睿, 马越飞, 海艺蕊, 刘凯栋, 刘万茂, 孙颖. 玉米根尖及整根响应缺铁的转录组比较研究[J]. 作物学报, 2026, 52(4): 1006-1021.
[12] 张超, 郭欢, 李忠玲, 岳淑宁, 赵娜. 基于BSA-seq技术定位玉米籽粒花青素关联基因[J]. 作物学报, 2026, 52(3): 780-789.
[13] 郭向阳, 涂亮, 王栋, 刘鹏飞, 王安贵, 易强, 任洪, 李刚, 祝云芳, 吴迅, 蒋喻林, 田丰, 陈泽辉. 热带Suwan种质在我国玉米种质改良中的创新与利用[J]. 作物学报, 2026, 52(3): 655-664.
[14] 马亮, 马璐, 张舒钰, 章慧敏, 王仁明, 宋旭东, 张振良, 冒宇翔, 陆虎华, 陈国清, 郝德荣, 周广飞. 玉米苞叶数目转录组分析及候选基因鉴定[J]. 作物学报, 2026, 52(3): 790-801.
[15] 孟成, 王哲. 玉米ZmPFK基因家族全基因组鉴定及响应胁迫表达分析[J]. 作物学报, 2026, 52(3): 764-779.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2