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作物学报 ›› 2022, Vol. 48 ›› Issue (7): 1669-1682.doi: 10.3724/SP.J.1006.2022.14111

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

马铃薯储藏期花青素变化及合成相关基因表达分析

李洁雅1(), 李红艳1(), 叶广继1,2,3,4,5,*(), 苏旺1,2,3,4,5, 孙海宏1,2,3,4,5, 王舰1,2,3,4,5,*()   

  1. 1青海大学, 青海西宁 810016
    2青海省农林科学院, 青海西宁 810016
    3青海大学青藏高原生物技术教育部重点实验室, 青海西宁 810016
    4青海省马铃薯育种重点实验室, 青海西宁 810016
    5省部共建三江源生态与高原农牧业国家重点实验室, 青海西宁 810016
  • 收稿日期:2021-06-25 接受日期:2021-10-19 出版日期:2022-07-12 网络出版日期:2022-05-10
  • 通讯作者: 叶广继,王舰
  • 作者简介:李洁雅, E-mail: 17453345@qq.com
    李红艳, E-mail: 1286068155@qq.com第一联系人：

    ** 同等贡献

  • 基金资助:
    财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-9);专用型马铃薯产业高质量发展关键技术研发与示范基金项目(2019-NK-A1)

Changes of anthocyanins and expression analysis of synthesis-related genes in potato during storage period

LI Jie-Ya1(), LI Hong-Yan1(), YE Guang-Ji1,2,3,4,5,*(), SU Wang1,2,3,4,5, SUN Hai-Hong1,2,3,4,5, WANG Jian1,2,3,4,5,*()   

  1. 1Qinghai University, Xining 810016, Qinghai, China
    2Qinghai Academy of Agriculture and Forestry, Xining 810016, Qinghai, China
    3Key Laboratory of Biotechnology, Ministry of Education Qinghai-Tibet Plateau, Qinghai University, Xining 810016, Qinghai, China
    4Key Laboratory of potato breeding Qinghai Province, Xining 810016, Qinghai, China
    5Ministry of State Key Laboratory of Sanjiangyuan Ecology and Plateau Agriculture and Animal Husbandry, Xining 810016, Qinghai, China
  • Received:2021-06-25 Accepted:2021-10-19 Published:2022-07-12 Published online:2022-05-10
  • Contact: YE Guang-Ji,WANG Jian
  • About author:First author contact:

    ** Contributed equally to this work

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摘要：

目前植物花青素合成机理研究深入, 但花青素降解机制及组分变化研究鲜少。储藏过程对彩色马铃薯品质影响较大, 然而关于储藏期间马铃薯块茎中花青素含量及组分变化的规律及机制研究尚不完全清楚。本试验测定了不同储藏期间的2种彩色马铃薯的花青素含量、组分, 还原糖含量。同时, 利用qRT-RCR对花青素代谢相关基因(StPAL、StC4H、St4CL、StF3’H、StDFR、StUFGT、StF3’5’H、StAN1和StbHLH1)表达量进行分析。结果表明, 随着储藏时间延长, 花青素的总含量下降, 主要组分减少, 所有基因表达量均降低(黑金刚中StDFR例外), 变化规律与花青素的降解规律基本一致; 还原糖储藏期间缓慢升高可能影响花青素变化。本试验研究结果对彩色马铃薯储藏期间花青素含量的保持具有指导意义, 可为彩色马铃薯品种的选育及资源的合理利用提供理论参考。

关键词: 彩色马铃薯, 储藏期间, 基因表达, 花青素

Abstract:

Researches on the biosynthesis mechanism of anthocyanins have been widely done in plants, while little information is available on the mechanism of anthocyanins degradation and compositions in anthocyanins. The storage period affects the quality of potatoes. During storage period, the changes in anthocyanins content and composition of colored potato tubers remains unclear. In this study, the contents and components of anthocyanins and reducing sugar content were measured during storage period. Additionally, the relative expression of anthocyanins metabolism related genes (StPAL, StC4H, St4CL, StF3’H, StDFR, StUFGT, StF3’5’H, StAN1, and StbHLH1) were analyzed using qRT-RCR. The results showed that with the extension of storage period, the total contents and components of anthocyanins decreased. The relative expression levels of anthocyanins metabolism-related genes were down-regulated, but not StDFR in Heijingang. During storage period, the elevated reducing sugar might affect the anthocyanin content. Overall, these results indicated that a high level of anthocyanin during storage contributed to the high-quality colored potato, providing theoretical basis for breeding new colored potato varieties and manufacture products in future.

Key words: colored potato, storage period, gene relative expression, anthocyanins

图1

植物花青素生物合成途径[3]"

表1

花青素合成相关的qRT-PCR引物"

基因名称
Gene name		 正向引物
Forward primer (5°-3°)		 反向引物
Reverse primer (5°-3°)
StPAL GCAATGTGCAATGGACAGATTA GGCAAACATTTAGCAGATTGGA
StC4H GAGAGATCAACGAGGATAACGT CCACTCAATTGACCACAATGTT
St4CL TACTTGAAGCACGACGATGTGGTG GAACTACAGCAGCGACTGAGACAC
StANS ATTCCATCTGTTTTCAGTTCGC TACTCGTCACATTTCACTTCGA
StF3'5'H CCTATGGACCTCGTTGGAAGTTGC GCTCATTGGCACGAACATTGGC
StUFGT TGTGTTGCATCCTAGTTACGGA TCGAAGAGACAGGAGGCTCG
StDFR TGTCCATGCTACTGTTCGTGATCC GCTTCCTCCACTGCCAAGTCTG
StF3’H GGCTCGTTGTGGAATCTGACCTG TCTCACAGCTCGGATGCAATTC
StbHLH1 ACATCACAGGCAGCTGAAGTT GTTTGCTATCATCTGVACCCCA
StAN1 CACCCTTATCCGCCTACAAA TTCTTCTCGGCTCCACTTCA
X83206 GGATGCTTACGCTGGATGGAATGC TTCCGGTGTGGTTGGATTCTGTTC

图2

不同彩色马铃薯资源横切图"

图3

不同彩色马铃薯资源花青素含量"

图4

储藏期间2种彩色马铃薯块茎颜色变化"

图5

储藏期间两种彩色马铃薯的花青素含量变化"

表2

马铃薯花青素组分分析"

品种
Variety		 时期
Phase		 飞燕草色素
Delphinidin		 矢车菊色素
Cyanidin		 矮牵牛色素
Petunidin chloride		 天竺葵色素
Pelargonidin		 芍药素
Peonidin		 锦葵色素
Malvidin		 总计
Total
黑金刚
Heijingang		 前期Prophase (7 d) 2.19 3.37 39.46 3.17 35.21 4.13 87.33
后期Later period (35 d) 1.31# 1.03# 18.98# 2.01# 12.51# 2.34# 30.98
红美人
Hongmeiren		 前期Prophase (7 d) 5.91 73.75 7.65 87.31
后期Later period (35 d) 1.22# 6.12# 3.91# 10.57

图6

‘黑金刚’马铃薯不同储藏时间的高效液相色谱图 a: ‘黑金刚’马铃薯储藏前期样品; c: ‘黑金刚’马铃薯储藏后期样品; b和d: 6种花青素标准品。*, P<0.05。"

图7

‘红美人’马铃薯不同储藏时间的高效液相色谱图 a: ‘红美人’马铃薯储藏前期样品; c: ‘红美人’马铃薯储藏后期样品; b和d: 6种花青素标准品。*, P<0.05。"

表3

花青素的含量与花青素基因表达量的相关分析"

品种
Variety		 相关系数
Correlation
coefficient		 y x1 x2 x3 x4 x5 x6 x7 x8 x9
黑金刚
Heijingang		 x1 0.99 1.00 0.67 0.96 -0.84 0.74 0.85 0.82 0.87 0.88
x2 0.71 0.67 1.00 0.72 -0.95 0.95 0.92 0.82 0.87 0.73
x3 0.95 0.96 0.72 1.00 -0.83 0.78 0.87 0.71 0.83 0.93
x4 -0.86 -0.84 -0.95 -0.83 1.00 -0.96 -0.97 -0.94 -0.97 -0.80
x5 0.72 0.74 0.95 0.78 -0.96 1.00 0.98 0.85 0.95 0.67
x6 0.83 0.85 0.92 0.87 -0.97 0.98 1.00 0.87 0.98 0.75
x7 0.81 0.82 0.82 0.71 -0.94 0.85 0.87 1.00 0.95 0.66
x8 0.84 0.87 0.87 0.83 -0.97 0.95 0.98 0.95 1.00 0.71
x9 0.94 0.88 0.73 0.93 -0.80 0.67 0.75 0.66 0.71 1.00
红美人
Hongmeiren		 x1 0.86 1.00 0.61 0.89 0.90 0.15 0.94 0.22 0.86 0.95
x2 0.59 0.61 1.00 0.64 0.50 -0.09 0.82 0.87 0.86 0.67
x3 0.99 0.89 0.64 1.00 0.98 -0.11 0.86 0.21 0.88 0.98
x4 0.97 0.90 0.50 0.98 1.00 -0.08 0.81 0.04 0.81 0.97
x5 0.98 0.88 0.56 0.98 0.98 1.00 0.85 0.15 0.87 0.98
x6 0.86 0.94 0.82 0.86 0.81 0.21 1.00 0.53 0.98 0.93
x7 0.19 0.22 0.87 0.21 0.04 0.07 0.53 1.00 0.59 0.27
x8 0.89 0.86 0.86 0.88 0.81 0.16 0.98 0.59 1.00 0.93
x9 0.98 0.95 0.67 0.98 0.97 0.04 0.93 0.27 0.93 1.00

图8

储藏期间彩色马铃薯花青素相关基因表达量"

图9

储藏期间彩色马铃薯还原糖含量"

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