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作物学报 ›› 2023, Vol. 49 ›› Issue (8): 2160-2170.doi: 10.3724/SP.J.1006.2023.24190

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

栽培种花生单仁重QTL定位分析

李星, 杨会, 骆璐, 李华东, 张昆, 张秀荣, 李玉颖, 于海洋, 王天宇, 刘佳琪, 王瑶, 刘风珍(), 万勇善()   

  1. 山东农业大学农学院 / 作物生物学国家重点实验室, 山东泰安 271018
  • 收稿日期:2022-08-16 接受日期:2023-02-10 出版日期:2023-08-12 网络出版日期:2023-03-02
  • 通讯作者: 刘风珍,万勇善
  • 作者简介:E-mail: 2683463925@qq.com
  • 基金资助:
    山东省花生产业技术体系项目(SDAIT-04-03);山东省农业良种工程项目(2020LZGC001);山东省重点研发计划项目(2019GNC106002);财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13)

QTLs mapping for single-seed weight of cultivated peanut

LI Xing, YANG Hui, LUO Lu, LI Hua-Dong, ZHANG Kun, ZHANG Xiu-Rong, LI Yu-Ying, YU Hai-Yang, WANG Tian-Yu, LIU Jia-Qi, WANG Yao, LIU Feng-Zhen(), WAN Yong-Shan()   

  1. College of Agronomy, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, Shandong, China
  • Received:2022-08-16 Accepted:2023-02-10 Published:2023-08-12 Published online:2023-03-02
  • Contact: LIU Feng-Zhen,WAN Yong-Shan
  • Supported by:
    Construction Project of Shandong Peanut Industrial Technology System(SDAIT-04-03);Shandong Agricultural Improved Varieties Engineering Project(2020LZGC001);Shandong Provincial Key Research and Development Program(2019GNC106002);China Agriculture Research System of MOF and MARA(CARS-13)

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摘要：

花生(Arachis hypogaea L.)是我国重要的油料作物和经济作物, 单仁重是决定花生产量和商品性的重要性状之一。本研究以大粒品种山花15号(母本)与小粒品种中花12号(父本)杂交构建的RIL群体为材料, 基于高密度遗传图谱, 在6个种植环境下进行单仁重QTL定位。在A04、A06、A07、B05、B07、B09和B10等7个染色体上共检测到9个与单仁重相关的QTL, 这些QTL的LOD值为3.01~33.97、表型贡献率为2.61%~34.28%、加性效应值为-0.03~0.15 g、定位的物理区间为0.03~4.32 Mb, 其中, qSSWA07.1是在6个种植环境下重复检测的单仁重主效QTL, qSSWA06.1和qSSWB09.1均在4个种植环境下重复检测到。qSSWA07.1和qSSWA06.1的加性效应为正, 增效位点来自山花15号, qSSWB09.1的加性效应为负, 增效位点来自中花12号, 利用与qSSWA06.1、qSSWA07.1和qSSWB09.1紧密连锁的3个bin标记(A06: Block3344、A07: Block3373和B09: Block10032)的基因型分析了3个位点调控花生单仁重的累加效应, 发现携带3个增效等位基因的家系的单仁重最大, 而不携带增效等位基因的家系的单仁重最小。对qSSWA06.1、qSSWA07.1和qSSWB09.1标记区间内的注释基因进行KEGG代谢通路富集分析, 结合基因的功能注释和在不同花生组织中的表达量差异, 预测了4个候选基因(Arahy.9UY90I、Arahy.RX7YKY、Arahy.3ZC2CN和Arahy.9V2WXE)。研究结果为花生产量相关性状的遗传改良提供了参考。

关键词: 花生, 单仁重, QTL定位, 候选基因

Abstract:

Peanut (Arachis hypogaea L.) is an important oil and cash crop in China, and the single-seed weight is one of the important traits that determine the yield and commodity of peanut. In this study, QTLs mapping for single-seed weight of peanut was performed using RIL population constructed from a cross between large-kernel variety Shanhua 15 (female) and small-kernel variety Zhonghua 12 (male), based on a high-density genetic map. Nine QTLs related to single-seed weight were identified on seven chromosomes (A04, A06, A07, B05, B07, B09, and B10). The LOD values of these QTLs was 3.01-33.97, phenotypic variation contribution rates was 2.61%-34.28%, the additive effect values were -0.03 to 0.15 g, and the physical range of positioning was 0.03-4.32 Mb. qSSWA07.1 was a stable major QTL repeatedly detected in six planting environments, and qSSWA06.1 and qSSWB09.1 were repeatedly detected in four planting environments. The additive effect of qSSWA07.1 and qSSWA06.1 were positive, and the favorable alleles were inherited from Shanhua 15. The additive effect of qSSWB09.1 was negative, and the favorable allele was inherited from Zhonghua 12. The additive effect of qSSWA06.1, qSSWA07.1, and qSSWB09.1 on peanut single-seed weight was analyzed with the genotype of three bin markers (A06: Block3344, A07: Block3373, and B09: Block10032) closely-linked with QTLs. The mean value of single-seed weight of lines with three favorable alleles was the largest, and that of the lines without the favorable alleles was the smallest. The candidate genes in qSSWA06.1, qSSWA07.1, and qSSWB09.1 were analyzed by KEGG enrichment pathway, according to the expression in different tissues and functional annotation of these genes, four candidate genes were predicted, Arahy.9UY90I, Arahy.RX7YKY, Arahy.3ZC2CN, and Arahy.9V2WXE, which may play a significant role in the regulation of peanut seed growth and development. The results provided the reference for the genetic improvement of yield related traits in peanut.

Key words: peanut, single-seed weight, QTLs mapping, candidate genes

表1

亲本及RIL群体单仁重性状统计分析"

种植环境
Planting environment		 亲本Parent RIL群体 RIL population
山花15号
Shanhua 15		 中花12号
Zhonghua 12		 最大值
Max.		 最小值
Min.		 平均值
Mean		 标准差
SD		 变异系数
CV (%)		 偏度
Skewness		 峰度
Kurtosis
2014 1.39 0.71** 1.63 0.41 0.93 0.25 26.88 0.291 -0.413
2015 1.21 0.58** 1.33 0.22 0.74 0.18 24.32 0.283 0.286
2016 1.08 0.42** 1.24 0.24 0.64 0.18 28.13 0.414 0.217
2017 1.14 0.75** 1.52 0.59 0.94 0.19 20.21 0.354 -0.209
2020 1.18 0.76** 1.57 0.48 0.97 0.21 21.68 0.116 -0.543
2021 1.16 0.76** 1.21 0.33 0.76 0.17 22.37 0.027 -0.437

图1

花生RIL群体单仁重频次分布 2014、2015、2016、2017、2020、2021代表不同的种植环境。RIL: 重组自交系。"

图2

花生单仁重相关QTL基因组分布2014、2015、2016、2017、2020、2021代表种植环境。A04、A06、A07、B05、B07、B09、B10代表不同染色体。"

表2

花生单仁重相关QTL信息"

QTL 种植环境
Planting environment		 染色体
Chr.		 标记区间
Marker interval		 物理区间长度
Physical interval length (Mb)		 阈值
LOD		 贡献率
PVE (%)		 加性效应
ADD (g)
qSSWA04.1 2015 A04 Block1622-Block1625 2.05 3.01 3.41 -0.03
qSSWA06.1 2021 A06 Block3360-Block3309 4.32 6.82 5.83 0.05
2020 8.30 7.61 0.05
2017 10.24 9.40 0.06
2014 8.11 7.39 0.07
qSSWA07.1 2021 A07 Block3421-Block3366 1.39 31.02 32.30 0.10
2020 33.62 34.06 0.11
2017 33.97 34.28 0.12
2016 13.75 16.36 0.07
2015 18.57 21.34 0.08
2014 30.17 31.34 0.15
qSSWB05.1 2020 B05 Block7608-Block7556 0.84 3.12 2.92 0.04
qSSWB05.2 2017 B05 Block7651-Block7587 3.01 4.34 3.33 0.03
qSSWB07.1 2014 B07 Block8272-Block8264 0.40 3.80 4.12 0.05
qSSWB07.2 2020 B07 Block8284-Block8285 0.03 3.08 2.61 0.04
qSSWB09.1 2021 B09 Block10054-Block10004 1.45 5.27 4.21 -0.04
2020 5.79 4.48 -0.04
2017 7.12 6.38 -0.04
2014 6.30 5.39 -0.06
qSSWB10.1 2015 B10 Block10084-Block10083 0.58 3.16 3.36 0.03

图3

qSSWA06.1、qSSWA07.1和 qSSWB09.1在群体中的表型效应 2014、2015、2016、2017、2020、2021代表不同的种植环境; AABBCC、AAbbcc、AABBcc、AAbbCC、aaBBCC、aaBBcc、aabbCC、aabbcc代表不同基因型。"

图4

花生单仁重QTL区间候选基因KEGG富集通路分析 A07、B09和A06代表不同的染色体。"

图5

12个候选基因在花生不同组织中的差异表达 颜色越深代表表达量越高, 反之越低。"

表3

qSSWA06.1、qSSWA07.1和qSSWB09.1的候选基因分析"

基因 ID
Gene ID		 染色体
Chr.		 物理区间
Physical interval (bp)		 基因家族
Gene family		 功能注释
Functional description
Arahy.9UY90I A07 447423-452848 legfed_v1_0.L_MWNP2J 烯醇酶
Enolase
Arahy.KE1FF5 A07 955911-958635 legfed_v1_0.L_KG5CQL 半胱氨酸合酶/半胱氨酸β合酶
Cysteine synthase/cystathionine β-synthase
Arahy.K4CMZR A07 1001166-1002296 legfed_v1_0.L_1XK45N 丝氨酸O-乙酰转移酶
Serine O-acetyltransferase
Arahy.K0DIZR A07 1218259-1224643 legfed_v1_0.L_0GKWV0 ABC转运蛋白1型
ABC transporter type 1
Arahy.RX7YKY B09 157391371-157396195 legfed_v1_0.L_BH5DLF 甜菜碱同型半胱氨酸甲基转移酶
Betaine-homocysteine methyltransferase
Arahy.E9249P B09 157550706-157553539 legfed_v1_0.L_0C7SKC 类RmlC-like cupins蛋白
Proteins similar to the RmlC-like cupins
Arahy.IQP7TJ B09 157714814-157717184 legfed_v1_0.L_FMT5KJ 糖苷水解酶
Glycoside hydrolase
Arahy.QJT344 A06 110434786-110446942 legfed_v1_0.L_9WXLVF 泛素特异性蛋白酶
Ubiquitin specific proteases
Arahy.3ZC2CN A06 110489370-110491090 legfed_v1_0.L_63KDF3 LSM蛋白质
LSM proteins
Arahy.S90AI0 A06 112231732-112241188 legfed_v1_0.L_J3GGX7 核转录和剪接因子
Nuclear transcription and splicing factors
Arahy.57RIKC A06 110715861-110721136 legfed_v1_0.L_F86644 磷酸吡哆醛依赖性转移酶
Pyridoxal phosphate-dependent transferase
Arahy.9V2WXE A06 111734794-111743008 legfed_v1_0.L_RNPQZ0 真核翻译起始因子
Eukaryotic translation initiation factor
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