纤毛鹅观草-普通小麦高抗条锈病多年生属间杂种F1的创制及鉴定

doi:10.3724/SP.J.1006.2024.31056

作物学报 ›› 2024, Vol. 50 ›› Issue (6): 1406-1420.doi: 10.3724/SP.J.1006.2024.31056

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

纤毛鹅观草-普通小麦高抗条锈病多年生属间杂种F₁的创制及鉴定

朱明昆¹(), 包俊浩¹, 庞菁璐¹, 周诗绮¹, 方忠艳¹, 郑文¹, 张亚洲¹^,², 吴丹丹¹^,²^,^*()

¹四川农业大学小麦研究所, 四川成都 611130
²西南作物基因资源发掘与利用国家重点实验室, 四川成都 611130

收稿日期:2023-10-08 接受日期:2024-01-31 出版日期:2024-06-12 网络出版日期:2024-02-27
通讯作者: * 吴丹丹, E-mail: 14646@sicau.edu.cn
作者简介:E-mail: zhumk2125@163.com
基金资助:
国家自然科学基金青年项目(32200180);四川省中央引导地方科技发展专项(2023ZYD0088);四川省教育厅项目(2022YFSY0035)

Generation and identification of a resistance to stripe rust perennial intergeneric hybrid F₁ between Roegneria ciliaris and common wheat

ZHU Ming-Kun¹(), BAO Jun-Hao¹, PANG Jing-Lu¹, ZHOU Shi-Qi¹, FANG Zhong-Yan¹, ZHENG Wen¹, ZHANG Ya-Zhou¹^,², WU Dan-Dan¹^,²^,^*()

¹Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
²State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Chengdu 611130, Sichuan, China

Received:2023-10-08 Accepted:2024-01-31 Published:2024-06-12 Published online:2024-02-27
Contact: * E-mail: 14646@sicau.edu.cn
Supported by:
Youth Fund of the National Natural Science Foundation of China(32200180);Special Projects of the Central Government in Guidance of Local Science and Technology Development(2023ZYD0088);Department of Education Project of Sichuan Province(2022YFSY0035)

摘要/Abstract

摘要：

本研究对鹅观草属(Roegneria C. Koch.) 13个物种29份材料进行条锈病田间鉴定和抗条锈病基因检测, 将筛选的抗病材料与小麦进行人工属间杂交, 并对属间杂种F₁进行形态学、细胞遗传学及小麦条锈病抗性检测, 结果显示: 82.76%的材料在田间对条锈病表现中抗以上的抗性, 且均含有5个以上的已知抗条锈病基因的等位基因, 但仍可能存在条锈病抗性新基因; 筛选到的一份高抗条锈病的纤毛鹅观草(Roegneria ciliaris [Trin.] Nevski) ZY11004-R, 将其与5个普通小麦(Triticum aestivum L.)品种进行人工属间杂交, 通过胚拯救的方式成功获得了纤毛鹅观草-普通小麦CSph2a属间杂种F₁; 杂种F₁体细胞染色体条数为35条(基因组组成为StYABD), 花粉母细胞减数分裂I中期染色体多为单价体, 形态特征处于两亲本之间, 但在生活习性上获得多年生的性状, 并表现出高抗条锈病。

关键词: 鹅观草属, 条锈病, 属间杂种, 远缘杂交, 多年生小麦

Abstract:

In this study, we evaluated the stripe rust resistance type and estimated the published resistance genes in 13 Roegneria species including 29 materials. Afterward, we conducted artificial crosses and investigated the morphology characters, genome constitution, and stripe rust resistance of the intergeneric hybrid F₁ between stripe rust resistance Roegneria material and common wheat. The results showed as follows: 82.76% of the 29 tested materials exhibited stripe rust resistance at adult stage, and they contained more than 5 alleles of stripe rust resistance genes, which might still carry new resistance genes to wheat stripe rust. An intergeneric hybrids R. ciliaris-CSph2a F₁was generated using the screened high stripe rust resistance Roegneria ciliaris [Trin.] Nevski (ZY11004-R) and common wheat mutant CSph2a, and raised based on the embryo rescue technology. F₁ hybrid contained 35 chromosomes with StYABD genome constitution and an average of 26.84 monovalents during pollen mother cell metaphase I. Besides, F₁ exhibited morphological intermediacy, except the perennial living was inherited form from maternal R. ciliaris with high resistance to wheat stripe rust.

Key words: Roegneria, stripe rust, intergeneric hybrid, distant hybridization, perennial wheat

朱明昆, 包俊浩, 庞菁璐, 周诗绮, 方忠艳, 郑文, 张亚洲, 吴丹丹. 纤毛鹅观草-普通小麦高抗条锈病多年生属间杂种F₁的创制及鉴定[J]. 作物学报, 2024, 50(6): 1406-1420.

ZHU Ming-Kun, BAO Jun-Hao, PANG Jing-Lu, ZHOU Shi-Qi, FANG Zhong-Yan, ZHENG Wen, ZHANG Ya-Zhou, WU Dan-Dan. Generation and identification of a resistance to stripe rust perennial intergeneric hybrid F₁ between Roegneria ciliaris and common wheat[J]. Acta Agronomica Sinica, 2024, 50(6): 1406-1420.

图/表 12

表1

供试材料"

物种 Species	编号 Accession	倍性 Ploidy	基因组组成 Genome constitution		生活型 Life form	分布/来源 Distribution/Origin
R. barbicalla (毛盘鹅观草)	ZY11089	2n = 4x = 28	StStYY		多年生Perennial	宁夏银川 Yinchuan, Ningxia
R. ciliaris (纤毛鹅观草)	ZY11004	2n = 4x = 28	StStYY		多年生Perennial	陕西临潼 Lintong, Shaanxi
R. ciliaris	ZY1008	2n = 4x = 28	StStYY		多年生Perennial	浙江嵊州 Shengzhou, Zhejiang
R. ciliaris	Z98050	2n = 4x = 28	StStYY		多年生Perennial	四川汶川 Wenchuan, Sichuan
R. ciliaris	Z98058	2n = 4x = 28	StStYY		多年生Perennial	四川崇州 Chongzhou, Sichuan
R. ciliaris	ZY276395	2n = 4x = 28	StStYY		多年生Perennial	四川红原 Hongyuan, Sichuan
R. ciliaris	88-89-228	2n = 4x = 28	StStYY		多年生Perennial	陕西杨凌 Yangling, Shaanxi
R. ciliaris	88-89-236	2n = 4x = 28	StStYY		多年生Perennial	四川雅安 Ya’an, Sichuan
R. ciliaris	88-89-238	2n = 4x = 28	StStYY		多年生Perennial	黑龙江哈尔滨 Harbin, Heilongjiang
R. ciliaris	88-89-294	2n = 4x = 28	StStYY		多年生Perennial	河南郑州 Zhengzhou, Henan
R. dolichathera (长芒鹅观草)	ZY230025	2n = 4x = 28	StStYY		多年生Perennial	四川天全 Tianquan, Sichuan
R. hondai (本田鹅观草)	ZY11020	2n = 4x = 28	StStYY		多年生Perennial	内蒙古呼和浩特Hohhot, Inner Mongolia
R. japonesis (竖立鹅观草)	88-89-252	2n = 4x = 28	StStYY		多年生Perennial	四川宜宾 Yibin, Sichuan
R. japonesis	88-89-263	2n = 4x = 28	StStYY		多年生Perennial	四川宜宾Yibin, Sichuan
R. japonesis	88-89-261	2n = 4x = 28	StStYY		多年生Perennial	四川宜宾Yibin, Sichuan
R. japonesis	88-89-253	2n = 4x = 28	StStYY		多年生Perennial	四川兴文 Xingwen, Sichuan
R. japonesis	88-89-242	2n = 4x = 28	StStYY		多年生Perennial	四川雅安 Ya’an, Sichuan
R. nakaii (吉林鹅观草)	ZY11027	2n = 4x = 28		StStYY	多年生Perennial	内蒙古呼和浩特 Hohhot, Inner Mongolia
R. pendulina (缘毛鹅观草)	ZY11006	2n = 4x = 28		StStYY	多年生Perennial	陕西临潼 Lintong, Shaanxi
R. pendulina	ZY11046	2n = 4x = 28		StStYY	多年生Perennial	内蒙古呼和浩特 Hohhot, Inner Mongolia
R. sinica (中华鹅观草)	ZY11021	2n = 4x = 28		StStYY	多年生Perennial	内蒙古呼和浩特 Hohhot, Inner Mongolia
R. sinica	ZY11029	2n = 4x = 28		StStYY	多年生Perennial	内蒙古包头 Baotou, Inner Mongolia
R. scabridula (粗糙鹅观草)	ZY11012	2n = 4x = 28		StStYY	多年生Perennial	内蒙古呼和浩特 Hohhot, Inner Mongolia
R. stricta (肃草)	ZY11083	2n = 4x = 28		StStYY	多年生Perennial	内蒙古阿拉善 Alxa, Inner Mongolia
R. stricta	ZY11030	2n = 4x = 28		StStYY	多年生Perennial	内蒙古呼和浩特 Hohhot, Inner Mongolia
R. tibetica (西藏鹅观草)	ZY230027	2n = 4x = 28		StStYY	多年生Perennial	西藏 Xizang
R. turczaninovii (直穗鹅观草)	ZY220095	2n = 4x = 28		StStYY	多年生Perennial	四川红原 Hongyuan, Sichuan
R. varia (多变鹅观草)	ZY11092	2n = 4x = 28		StStYY	多年生Perennial	宁夏银川 Yinchuan, Ningxia
T. aestivum cv. Kaixianluohanmai (KL)	—	2n = 6x = 42		AABBDD	一年生Annual	四川农业大学 Sichuan Agricultural University
T. aestivum cv. Chinese Spring (CS)	—	2n = 6x = 42		AABBDD	一年生Annual	四川农业大学 Sichuan Agricultural University
T. aestivum (CSph1b)	—	2n = 6x = 42		AABBDD	一年生Annual	四川农业大学 Sichuan Agricultural University
T. aestivum (CSph2b)	—	2n = 6x = 42		AABBDD	一年生Annual	四川农业大学 Sichuan Agricultural University
T. aestivum (CSph2a)	—	2n = 6x = 42		AABBDD	一年生Annual	四川农业大学 Sichuan Agricultural University

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Yr 基因 Yr gene	类型 Type	分子标记 Molecular marker	引物序列 Primer sequence (5′-3′)	参考文献 Reference
Yr5	DM	Yr5_B	GGGAACACTTCACGATCA AATTCCTTCATGCCTTCC	[38]
Yr9	SSR	P6M12-P	GTACTAGTATCCAGAGGTCACAAG CAGACAAACAGAGTACGGGC	[39]
Yr10	DM	Yr10-5	GGAAATGTGGCGGAGTACCA CGGAAGGGAGAACCACTGTC	[40]
Yr15	DM	WJL3F, WJL3R	AAAAGAGCTCGCCTCCTACG GCCATGATGAGATCGGGAGG	[41]
Yr17	SCAR	SC2372	AGGGGCTACTGACCAAGGCT TGCAGCTACAGCAGTATGTACACAAAA	[42]
Yr18	STS	csLV34	GTTGGTTAAGACTGGTGATGG TGCTTGCTATTGCTGAATAGT	[43]
Yr24	SSR	Xgwm273	ATTGGACGGACAGATGCTTT AGCAGTGAGGAAGGGGATC	[44]
Yr26	STS	Xwe173	GGGACAAGGGGAGTTGAAGC GAGAGTTCCAAGCAGAACAC	[45]
Yr28	DM	P175, P176	GCACCGTCCTTCATCTCAGT TGCTTTTCCCCGTATCCCTT	[46]
Yr39	SSR	Xgwm131	AATCCCCACCGATTCTTCTC AGTTCGTGGGTCTCTGATGG	[47]
Yr41	SSR	Xgwm410	GCTTGAGACCGGCACAGT CGAGACCTTGAGGGTCTAGA	[48]
Yr48	SSR	Xwmc727	CATAATCAGGACAGCCGCAC TAGTGGCCTGATGTATCTAGTTGG	[49]
Yr65	SSR	Xgwm18	GGTTGCTGAAGAACCTTATTTAGG TGGCGCCATGATTGCATTATCTTC	[50]
Yr67	SSR	Xbarc182	CCATGGCCAACAGCTCAAGGTCTC CGCAAAACCGCATCAGGGAAGCACCAAT	[51]
Yr84	SSR	P70	AATGGGAGGACTCTTGCGTG CTGGGAATGAACCGACAGCT	[52]

物种 Species	染色体数 Chromosome number	构型Configuration						交叉值^a Chiasmata/cell ^a	C值^b C-value ^b	统计数 Statistics
		I	II			III	IV
		I	Total	Ring	Rod	III	IV
CSph2a	42	0.18	20.82	17.88	2.94	0.06	—	38.88	0.93	32
R. ciliaris	28	0.13	13.93	13.77	0.17	—	—	27.70	0.99	30
(R. ciliaris×CSph2a) F₁	35	26.84	3.97	0	3.97	0.03	0.03	4.13	0.12	32

纤毛鹅观草-普通小麦高抗条锈病多年生属间杂种F₁的创制及鉴定

Generation and identification of a resistance to stripe rust perennial intergeneric hybrid F₁ between Roegneria ciliaris and common wheat

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序号 Ordinal number	物种 Species	编号 Accession	成株期感染型 Infection type in adult-plant stage (ITs = 0~9)	Yr 基因 Yr gene
1	R. ciliaris	ZY11004-R	0	Yr9+Yr17+Yr28+Yr39
2	R. pendulina	ZY11006	0	Yr9+Yr10+Yr15+Yr17+Yr18+Yr28+Yr39+Yr48
3	R. pendulina	ZY11046	0	Yr9+Yr10+Yr15+Yr17+Yr18+Yr39+Yr48
4	R. sinica	ZY11029	0	Yr10+Yr15+Yr17+Yr24+Yr28+Yr48
5	R. sinica	ZY11021	0	Yr10+Yr15+Yr17+Yr24+Yr28+Yr39
6	R. stricta	ZY11030	0	Yr9+Yr10+Yr17+Yr18+Yr28+Yr39
7	R. tibetica	ZY230027	0	Yr10+Yr17+Yr28+Yr39+Yr48
8	R. varia	ZY11092	0	Yr9+Yr10+Yr17+Yr28+Yr39+Yr48+Yr84
9	R. stricta	ZY11083	0	Yr9+Yr10+Yr17+Yr18+Yr28+Yr39
10	R. ciliaris	ZY1008	1	Yr9+Yr10+Yr17+Yr28+Yr39
11	R. ciliaris	Z98058	1	Yr9+Yr10+Yr17+Yr28+Yr39
12	R. dolichathera	ZY230025	1	Yr10+Yr17+Yr24+Yr28
13	R. nakaii	ZY11027	1	Yr10+Yr17+Yr24+Yr28
14	R. hondai	ZY11020	1	Yr10+Yr17+Yr24+Yr28+Yr39
15	R. ciliaris	ZY276395	2	Yr9+Yr10+Yr15+Yr17+Yr28+Yr39+Yr48
16	R. barbicalla	ZY11089	2	Yr9+Yr10+Yr15+Yr17+Yr26+Yr39+Yr84
17	R. turczaninovii	ZY220095	2	Yr10+Yr15+Yr17+Yr26+Yr28+Yr48
18	R. ciliaris	Z98050	4	Yr5+Yr9+Yr10+Yr17+Yr28+Yr39+Yr84
19	R. japonesis	88-89-252	4	Yr9+Yr10+Yr17+Yr28+Yr39+Yr48+Yr84
20	R. scabridula.	ZY11012	4	Yr9+Yr17+Yr28+Yr39
21	R. ciliaris	88-89-294	5	Yr5+Yr9+Yr10+Yr17+Yr24+Yr28+Yr39+Yr84
22	R. japonesis	88-89-263	5	Yr9+Yr10+Yr17+Yr28+Yr48+Yr84
23	R. japonesis	88-89-261	5	Yr9+Yr10+Yr17+Yr28+Yr39+Yr84
24	R. ciliaris	88-89-236	6	Yr9+Yr10+Yr17+Yr28+Yr39+Yr84
25	R. ciliaris	88-89-238	7	Yr9+Yr10+Yr17+Yr28
26	R. japonesis	88-89-253	7	Yr5+Yr9+Yr10+Yr17+Yr26+Yr28+Yr39+Yr48+Yr84
27	R. japonesis	88-89-242	7	Yr9+Yr10+Yr17+Yr28+Yr39+Yr48+Yr84
28	R. ciliaris	88-89-228	8	Yr9+Yr10+Yr17+Yr28+Yr39+Yr48+Yr84
29	R. ciliaris	ZY11004-S	9	Yr9+Yr10+Yr17+Yr28+Yr39

母本 Female	父本 Male	杂交小花数 Number of hybrid florets	子房膨大数 Ovary enlargement number	子房膨大率 Ovary enlargement rate (%)	出愈数 Number of callus generated	出愈率 Rate of callus generated (%)
ZY11004-R	CS	792	32	4.04	2	6.25
ZY11004-R	CSph1b	887	35	3.95	0	—
ZY11004-R	CSph2a	1872	108	5.77	1	0.93
ZY11004-R	CSph2b	2160	140	6.48	4	2.86
ZY11004-R	KL	1104	58	5.25	0	—
CS	ZY11004-R	280	0	0	0	—
CSph1b	ZY11004-R	286	1	0.35	0	—
CSph2a	ZY11004-R	242	3	1.24	0	—
CSph2b	ZY11004-R	423	1	0.24	0	—
KL	ZY11004-R	461	12	2.60	0	—
总计 Total		8507	390	4.55	7	1.79

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