渗透胁迫下烟草叶片基因的差异表达研究

作物学报 ›› 2007, Vol. 33 ›› Issue (06): 914-920.

渗透胁迫下烟草叶片基因的差异表达研究

汪耀富^1,2;杨天旭¹;刘国顺¹;赵春华¹;王佩¹;陈新建^1,*

1 河南农业大学国家烟草栽培生理生化研究基地，河南郑州450002；2 长沙卷烟厂博士后工作站，湖南长沙470007

收稿日期:2006-08-01 修回日期:1900-01-01 出版日期:2007-06-12 网络出版日期:2007-06-12
通讯作者: 陈新建

Differently Expressed Genes in Tobacco Leaves under Osmotic Stress

WANG Yao-Fu¹²,YANG Tian-Xu¹,LIU Guo-Shun¹,ZHAO Chun-Hua¹,WANG Pei¹,CHEN Xin-Jian^1*

1 National Research Center for Tobacco Cultivation, Physiology and Biochemistry, Henan Agricultural University, Zhengzhou 450002, Henan; 2 Postdoctoral Workstation of Changsha Cigarette Factory, Changsha 410007, Hunan, China

Received:2006-08-01 Revised:1900-01-01 Published:2007-06-12 Published online:2007-06-12
Contact: CHEN Xin-Jian

摘要/Abstract

摘要：

为了解干旱胁迫下烟草的抗旱机制，采用拟南芥基因芯片检测了PEG渗透胁迫（－1.2 MPa）48 h后烟草叶片中基因表达的变化。在31 182个基因微矩阵点中，有效差异表达（ratio值≥2或≤0.5）的基因为135个，上调50个，下调85个。其中包括一些具有防御功能的上调表达基因如两个普遍胁迫蛋白（USP）和与渗透调节物质海藻糖合成有关的基因ATTPS11及多个参与复制、转录和翻译等过程的基因MAPKK、bZIP、锌指蛋白、组蛋白His1-3、脱水响应DREB转录因子、WRKY转录因子家族、分子伴侣等，MAPKK具最大上调幅度，为3.94倍（序列号为At1g51660）。而参与光合作用的6个PSⅠ的光捕获复合体中lhca3和PSⅡ的光捕获复合体中核编码基因的lcb1、lcb2、lcb5、lcb6、lcb4.2的基因表达下调。还检测到38个未知基因，它们的差异表达可能跟渗透胁迫诱导有关，是我们下一步研究的重点。通过分析这些特异表达基因，揭示出一些潜在的生物学规律，为研究烟草逆境生理学提供了有价值的信息。

关键词: 烟草, 渗透胁迫, 差异表达, 基因芯片

Abstract:

Attempt to study the drought resistance defense mechanism in tobacco plant, the changes of gene expression in osmotic stressed tobacco (Nicotiana tabacum L.) leaves were analyzed by cDNA micro-array analysis. RNAs of tobacco leaves with or without (CK) PEG treatment for 48 h under －1.2 MPa were extracted and subjected to analysis of cDNA microarray based on Arabidopsis genomic sequence. There were about 135 differently expressed genes (with ratio values≥2 or ≤0.5) among 31 182 genes set in a microarray plate, in which 50 appeared to be up-regulated and 85 appeared to be down-regulated by osmotic stress. Functional analysis showed that some were defensive genes such as trehalose synthase (ATTPS11) and universal stress protein (USP), and the others were related to replication, transcription, translation and protein folding including bZIP family transcription factors, zinc finger proteins, histone H1-3, DREB1C, the WRKY, molecular chaperones and so on. All genes mentioned above were up-regulated by osmotic stress. Gene of MAPKK (At1g51660), a key player in signal transduction pathway, was up-regulated dramatically by 3.94 times. Among down-regulated genes there were 6 genes of light harvesting complex in photosystem Ⅰ and photosystem Ⅱ, including lhca3, lcb1, lcb2, lcb5, lcb6, and lcb4.2. The gene lcb6 (At1g15820) was most down-regulated by 3.9 times. What’s more, 38 unknown function genes were also detected in the experiment. The functions of those genes in plant osmotic stress response need to be elucidated in the future. By analyzing the differently expressed genes some valuable information underlining plant osmotic stress response were highlighted.

Key words: Tobacco, Osmotic stress, Different expression, cDNA microarray

汪耀富;杨天旭;刘国顺;赵春华;王佩;陈新建. 渗透胁迫下烟草叶片基因的差异表达研究[J]. 作物学报, 2007, 33(06): 914-920.

WANG Yao-Fu;YANG Tian-Xu;LIU Guo-Shun;ZHAO Chun-Hua;WANG Pei;CHEN Xin-Jian. Differently Expressed Genes in Tobacco Leaves under Osmotic Stress[J]. Acta Agron Sin, 2007, 33(06): 914-920.

参考文献

相关文章 15

[1]	李阿立, 冯雅楠, 李萍, 张东升, 宗毓铮, 林文, 郝兴宇. 大豆叶片响应CO₂浓度升高、干旱及其交互作用的转录组分析[J]. 作物学报, 2022, 48(5): 1103-1118.
[2]	李富, 王延周, 严理, 朱四元, 刘头明. 苎麻茎皮环状RNA表达谱分析[J]. 作物学报, 2021, 47(6): 1020-1030.
[3]	黄文功, 姜卫东, 姚玉波, 宋喜霞, 刘岩, 陈思, 赵东升, 吴广文, 袁红梅, 任传英, 孙中义, 吴建忠, 康庆华. 亚麻响应低钾胁迫转录谱分析[J]. 作物学报, 2021, 47(6): 1070-1081.
[4]	许静, 潘丽娟, 李昊远, 王通, 陈娜, 陈明娜, 王冕, 禹山林, 侯艳华, 迟晓元. 花生油脂合成相关基因的表达谱分析[J]. 作物学报, 2021, 47(6): 1124-1137.
[5]	王瑞莉, 王刘艳, 雷维, 吴家怡, 史红松, 李晨阳, 唐章林, 李加纳, 周清元, 崔翠. 结合RNA-seq分析和QTL定位筛选甘蓝型油菜萌发期与铝毒胁迫相关的候选基因[J]. 作物学报, 2021, 47(12): 2407-2422.
[6]	李鹏, 刘彻, 宋皓, 姚盼盼, 苏沛霖, 魏跃伟, 杨永霞, 李青常. 烟草非特异性脂质转移蛋白基因家族的鉴定与分析[J]. 作物学报, 2021, 47(11): 2184-2198.
[7]	任蒙蒙, 张红伟, 王建华, 王国英, 郑军. 玉米耐深播主效QTL qMES20-10的精细定位及差异表达基因分析[J]. 作物学报, 2020, 46(7): 1016-1024.
[8]	董庆园,马德清,杨学,刘勇,黄昌军,袁诚,方敦煌,于海芹,童治军,沈俊儒,许银莲,罗美中,李永平,曾建敏. 高抗黑胫病烤烟BAC文库的构建及分析[J]. 作物学报, 2020, 46(6): 869-877.
[9]	王晓阳,王丽媛,潘兆娥,何守朴,王骁,龚文芳,杜雄明. 亚洲棉短绒突变体纤维发育及其差异基因表达分析[J]. 作物学报, 2020, 46(5): 645-660.
[10]	衡友强,游西龙,王艳. 费尔干猪毛菜病程相关蛋白SfPR1a基因的异源表达增强了烟草对干旱、盐及叶斑病的抗性[J]. 作物学报, 2020, 46(4): 503-512.
[11]	杨梦婷, 张春, 王作平, 邹华文, 吴忠义. 玉米ZmbHLH161基因的克隆及功能研究[J]. 作物学报, 2020, 46(12): 2008-2016.
[12]	陈杉彬, 孙思凡, 聂楠, 杜冰, 何绍贞, 刘庆昌, 翟红. 甘薯IbCAF1基因的克隆及耐盐性、抗旱性鉴定[J]. 作物学报, 2020, 46(12): 1862-1869.
[13]	贾小霞,齐恩芳,刘石,文国宏,马胜,李建武,黄伟. AtDREB1A基因过量表达对马铃薯生长及抗非生物胁迫基因表达的影响[J]. 作物学报, 2019, 45(8): 1166-1175.
[14]	李乐晨,朱国忠,苏秀娟,郭旺珍. 适于海岛棉指纹图谱构建的SNP核心位点筛选与评价[J]. 作物学报, 2019, 45(5): 647-655.
[15]	马晓寒,张杰,张环纬,陈彪,温心怡,许自成. 通过外源MeJA抑制H₂O₂积累提高烟草的耐冷性[J]. 作物学报, 2019, 45(3): 411-418.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed