关键词: 穗茎注射法, 分子检测, 整合, 转基因小麦

Abstract:

Antisense phospholipase D_γ (PLD_γ) gene was introduced into LK906 wheat mediated by improved earstem injecting of Agrobacterium. The transformants were screened by PCR and PCR-Southern technique and the variation between transformation lines and receptor was analyzed. The results indicated that（1）400 bp special fragment to the plasmid was amplified in three plants（03039,03048,03050）, which showed that anti-PLD_γ gene was integrated into wheat genomes, thus the transgenic wheats of anti-PLD_γ gene were obtained in the world first. (2) There were distinct differences between transgenic lines and receptor in agronomic characters, such as plant height, 1 000-kernel weight and spike number per plant that were higher in transgenic lines than in receptor. Compared with in the receptor, in transgenic lines the plant height was from 79.2 cm to 83.8 cm and 13.4 cm higher; the average value of spike number per plant was 3.82 and higher significantly too; and 1 000-kernel weight was from 50.53 g to 52.98 g and 6.94 g higher. While the spike length, number of spikelet and grain number per spike were lower (Table 1). (3) The mature date of CK was on June tenth, those of transgenic plants (03048,03039,03050) were on June second, June third, June sixth respectively, and 4 to 8 days shorter than that of receptor. It showed that anti-PLDγ gene was good to advance mature date. Complete male sterile was observed in T₁ and the following generations and it would play an important role in heterosis utilization.

Key words: Earstem injecting, Molecular detection, Integration, Transgenic wheat