关键词: 小麦, 农杆菌转化, 基因型筛选, 瞬时表达, 分子检测

Abstract: Thirty-five Chinese spring wheat genotypes were tested for their sensitivity to C58c1 Agrobacterium strain’s (harboring a binary vector pPTN290 with nptⅡ gene and GUS gene) infection and final transformation. Six cultivars including PM97034, P187, Ba1401051, Yangmai 158, Xinchun 9 and Kefeng 6 were proved to be more sensitive than Bobwhite to the Agro-infection according to X-Gluc staining, with GUS gene transient expression frequency of 15.0% to 30.0%. Regeneration plants obtained from selection medium were identified by ELISA, PCR, leaf bleach and X-Gluc, and stable transformation events were obtained from Xinchun 9, PM97034 and Bobwhite by 3.3%, 2.0% and 1.1%, respectively. Southern blot analysis showed the alien genes integrated in most transgenic plants just by single copy. The alien genes in most T₁ transgenic lines segregated by Mendelian regulation, but the segregation ratio in a few T₁ transgenic lines was a little bit away from the expecting value, according to X²_c test. Xinchun 9 and PM97034 are suggested to be used in wheat Agrobacterium mediated transformation to replace Bobwhite for better result.

Key words: Triticum aestivum, Agrobacterium mediated transformation, Genotype screening, Transient expression, Molecular test