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作物学报 ›› 2008, Vol. 34 ›› Issue (09): 1644-1649.doi: 10.3724/SP.J.1006.2008.01644

• 耕作栽培·生理生化 • 上一篇    下一篇

小麦腺苷二磷酸葡萄糖焦磷酸化酶同工酶基因型与酶活性及淀粉含量的关系

岳向文1;赵法茂2;李天骄1;王宪泽1,*   

  1. 1 山东农业大学作物生物学国家重点实验室, 山东泰安271018; 2 泰山学院生物系, 山东泰安271021
  • 收稿日期:2007-11-22 修回日期:1900-01-01 出版日期:2008-09-12 网络出版日期:2008-09-12
  • 通讯作者: 王宪泽

Relationship of Isozyme Genotypes of AGP with Activity of AGP and Starch Content in Wheat Grain

YUE Xiang-Wen1,ZHAO Fa-Mao2,LI Tian-Jiao1,WANG Xian-Ze1*   

  1. 1 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, Shandong; 2 Department of Biology, Taishan College, Tai’an 271021, Shandong, China
  • Received:2007-11-22 Revised:1900-01-01 Published:2008-09-12 Published online:2008-09-12
  • Contact: WANG Xian-Ze

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1654

被引次数

5

摘要： 采用非变性聚丙烯酰胺凝胶电泳鉴定了我国60个代表性小麦品种的腺苷二磷酸葡萄糖焦磷酸化酶(AGP)同工酶基因型, 并测定了AGP活性及总淀粉含量, 以明确小麦籽粒AGP同工酶基因型组成及其与AGP活性和淀粉含量的关系。结果表明, AGP有AGPa、AGPb、AGPc和AGPd 4个等位基因位点, 其出现频率分别为96.7%、80.0%、86.7%和16.7%; 共检测到5种基因型, 其中基因型AGPabc出现频率最高, 为46.7%。不同基因型的品种间AGP活性和总淀粉含量差异显著(P<0.05)。其中具有基因型AGPabcd的品种酶活性及总淀粉含量最高, 表明小麦籽粒中不同AGP同工酶基因型对酶活性及淀粉含量有不同遗传效应。

关键词: 普通小麦, 腺苷二磷酸葡萄糖焦磷酸化酶, 基因型, 淀粉含量, 酶活性

Abstract: ADP-Glucose pyrophosphorylase(AGP) controls a rate-limiting step in the starch biosynthesis in developing wheat (Triticum aestivum L.) seeds, therefore, determinately affecting seed sink strength. Evidence is presented for the existence of AGP isozymes in common wheat endosperm. Following an approach already shown to be successful in maize (Zea mays L.), native polyacrylamide gel electrophoresis (native PAGE) was designed to identify AGP isozyme patterns from crude enzyme prepara-tions. To explore the relationship of isozyme genotypes with the starch content in wheat grains, activity of AGP and starch content were measured in 60 cultivars from 17 provinces in China. The results showed that there were four alleles, namely AGPa, AGPb, AGPc, and AGPd, with frequencies of 96.7%, 80.0%, 86.7%, and 16.7%, respectively. Five genotypes were determined in which AGPabc took on the highest frequency of 46.7% and AGPbc took on the lowest frequency of 3.3%. The activity of AGP and starch content in the cultivars with distinct genotypes were significantly different (P<0.05). The cultivars with genotype AGPabcd and AGPabc showed a high value of AGP activity and starch content. The lack of AGPa, AGPb, or AGPc in cultivars resulted in a low value of AGP activity and starch content. It indicated that different genotypes of AGP isozyme show distinct genetic impact on enzyme activity and starch content in common wheat.

Key words: Common wheat (Triticum aestivum L.), ADP-Glucose pyrophosphorylase, Genotype, Stanch content, Enzyme activity

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