江苏和东北粳稻稻米理化特性及Wx和OsSSIIa基因序列分析

doi:10.3724/SP.J.1006.2020.92043

作物学报 ›› 2020, Vol. 46 ›› Issue (6): 878-888.doi: 10.3724/SP.J.1006.2020.92043

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

江苏和东北粳稻稻米理化特性及Wx和OsSSIIa基因序列分析

赵春芳,岳红亮,田铮,顾明超,赵凌,赵庆勇,朱镇,陈涛,周丽慧,姚姝,梁文化,路凯,张亚东,王才林()

江苏省农业科学院粮食作物研究所 / 江苏省优质水稻工程技术研究中心 / 国家水稻改良中心南京分中心, 江苏南京210014

收稿日期:2019-08-13 接受日期:2020-01-15 出版日期:2020-06-12 网络出版日期:2020-01-23
通讯作者: 王才林
作者简介:E-mail: czhao@jaas.ac.cn
基金资助:
江苏省自然科学基金项目(BK20180302);江苏省农业科技自主创新资金(CX[18]1001);江苏省重点研发计划项目(BE2018357);国家现代农业产业技术体系建设专项(CARS-01-62);江苏省重点生物学实验室项目(4911707Z201705)

Physicochemical properties and sequence analysis of Wx and OsSSIIa genes in japonica rice cultivars from Jiangsu province and northeast of China

ZHAO Chun-Fang,YUE Hong-Liang,TIAN Zheng,GU Ming-Chao,ZHAO Ling,ZHAO Qing-Yong,ZHU Zhen,CHEN Tao,ZHOU Li-Hui,YAO Shu,LIANG Wen-Hua,LU Kai,ZHANG Ya-Dong,WANG Cai-Lin()

Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Jiangsu High Quality Rice Research and Development Center / Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, Jiangsu, China

Received:2019-08-13 Accepted:2020-01-15 Published:2020-06-12 Published online:2020-01-23
Contact: Cai-Lin WANG
Supported by:
Natural Science Foundation of Jiangsu Province(BK20180302);Jiangsu Agriculture Science and Technology Innovation Fund(CX[18]1001);Key Research and Development Programs of Jiangsu Province(BE2018357);China Agriculture Research System(CARS-01-62);Provincial Key Laboratory of Agrobiology Jiangsu Academy of Agricultural Sciences(4911707Z201705)

摘要/Abstract

摘要：

依据地理来源和直链淀粉含量将87份供试粳稻品种(系)划分为江苏普通粳稻、东北粳稻和江苏半糯粳稻3种类型, 系统比较了稻米理化性状及淀粉合成主效基因Wx和OsSSIIa序列的差异, 以探究其食味品质特征及其形成的分子基础。与东北粳稻相比, 江苏普通粳稻的直链淀粉含量偏低, 蛋白质含量和糊化温度偏高, 而胶稠度和RVA谱特征值相当。与江苏普通粳稻和东北粳稻相比, 江苏半糯粳稻具有较低的直链淀粉含量、较大的胶稠度及独特的RVA谱特征。大多数性状变异系数在3种类型粳稻中均较小, 仅消减值变异系数较大, 说明品种(系)间的稻米回生性存在较大差异而其他性状较为一致。各性状间的相关性在3种类型粳稻中表现不尽相同, 说明地理来源和品种特性影响食味品质性状间的相关程度。聚类分析将87份粳稻分为4个类群, 江苏普通粳稻与东北粳稻主要分布于类群I和II, 且品种间相互交错; 类群III由2个江苏普通粳稻品系和1个江苏半糯粳稻(徐稻9号)组成, 多数性状值位于普通粳稻与半糯粳稻之间; 类群IV中均为江苏半糯粳稻。序列分析表明江苏普通粳稻和东北粳稻中Wx ^b基因无序列差异, 而江苏半糯粳稻在Wx ^b第4外显子上发生G/A替换, 属Wx ^mp等位基因型; 江苏省部分粳稻品种(系)携带OsSSIIa(G-GC)等位基因, 从而导致高糊化温度。

关键词: 粳稻, 食味品质, 低直链淀粉含量, Wx, OsSSIIa

Abstract:

According to geographic origin and amylose content, 87 investigated japonica rice cultivars were divided into three types: Jiangsu common japonica rice, Northeast japonica rice and Jiangsu semi-waxy japonica rice. In order to explore the eating quality characteristics of the three types of japonica rice and their molecular basis, the physicochemical properties and the sequence of major genes Wx and OsSSIIa for starch synthesis were analyzed. Compared with Northeast japonica rice, Jiangsu common japonica rice had lower amylose content, higher protein content and gelatinization temperature, and similar gel consistency and RVA profiles. Compared with Northeast japonica rice and Jiangsu common japonica rice, Jiangsu semi-waxy japonica rice contained the lowest amylose content, the highest gel consistency and unique RVA profile. Variation coefficients of all characters except for setback value were small in three types of rice varieties, indicating that there was a great range of retrogradation within all the cultivars. The correlation coefficients among traits were different among three types of japonica rice, which indicated that the correlations were affected by the ecological conditions of different regions and properties of varieties. By cluster analysis, 87 japonica rice varieties (lines) were divided into four groups. Jiangsu common and Northeast japonica rice varieties (lines) were intersected in group I and group II. Group III was composed of two lines of Jiangsu common japonica rice and one semi-waxy variety (Xudao 9), and the most of their character values were ranged between those common japonica rice and semi-waxy japonica rice. The Jiangsu semi-waxy rice varieties (lines) with unique physicochemical characteristics were clustered into group IV. By sequence analysis, there was no difference in Wx ^b gene between Jiangsu common japonica rice and Northeast japonica rice, but in Jiangsu semi-waxy japonica rice G/A substitution occurred in Exon 4 of Wx ^b, which is the Wx ^mp allele. The higher gelatinization temperature in some of Jiangsu japonica rice varieties (lines) was controlled by the allele of OsSSIIa (G-GC).

Key words: japonica rice, eating quality, low amylose content, Wx, OsSSIIa

赵春芳,岳红亮,田铮,顾明超,赵凌,赵庆勇,朱镇,陈涛,周丽慧,姚姝,梁文化,路凯,张亚东,王才林. 江苏和东北粳稻稻米理化特性及Wx和OsSSIIa基因序列分析[J]. 作物学报, 2020, 46(6): 878-888.

ZHAO Chun-Fang,YUE Hong-Liang,TIAN Zheng,GU Ming-Chao,ZHAO Ling,ZHAO Qing-Yong,ZHU Zhen,CHEN Tao,ZHOU Li-Hui,YAO Shu,LIANG Wen-Hua,LU Kai,ZHANG Ya-Dong,WANG Cai-Lin. Physicochemical properties and sequence analysis of Wx and OsSSIIa genes in japonica rice cultivars from Jiangsu province and northeast of China[J]. Acta Agronomica Sinica, 2020, 46(6): 878-888.

图/表 9

表1

供试材料信息"

类型 Type	份数 Number	材料名称 Variety name
江苏普通粳稻 Jiangsu common japonica rice	34	武运5020、武运5051、淮稻5号、扬粳3012、武运粳32、武育粳3号、泗稻16、泗稻212、泗稻301、连粳16、江苏省农业科学院粮食作物研究所培育的普通粳稻品系24份 Wuyun 5020, Wuyun 5051, Huaidao 5, Yangjing 3012, Wuyunjing 32, Wuyujing 3, Sidao 16, Sidao 212, Sidao 301, Lianjing 16, 24 breeding lines of common japonica rice bred by the Institute of Food Crops, Jiangsu Academy of Agricultural Sciences
东北粳稻 Northeast japonica rice	32	稻花香2号、龙洋16、松粳22、松粳34、松香2号、五优稻1号、龙稻16、龙稻18、龙稻25、龙稻28、吉粳113、吉粳302、吉粳303、吉粳306、吉粳511、吉粳512、吉粳526、吉粳528、吉粳803、吉粳809、吉粳81、吉粳83、吉粳88、吉农引6号、沈农9903、长白19、长白20、昌粳1号、昌尹1号、辽粳337、辽粳399、铁粳11 Daohuaxiang 2, Longyang 16, Songjing 22, Songjing 34, Songxiang 2, Wuyoudao 1, Longdao 16, Longdao 18, Longdao 25, Longdao 28, Jijing 113, Jijing 302, Jijing 303, Jijing 306, Jijing 511, Jijing 512, Jijing 526, Jijing 528, Jijing 803, Jijing 809, Jijing 81, Jijing 83, Jijing 88, Jinongyin 6, Shennong 9903, Changbai 19, Changbai 20, Changjing 1, Changyin 1, Liaojing 337, Liaojing 399, Tiejing 11
江苏半糯粳稻 Jiangsu semi-waxy japonica rice	21	徐稻9号、宁粳8号、苏香粳100、南粳46、南粳5055、南粳9108、南粳2728、南粳505、南粳3908、南粳5718、南粳58、南粳晶谷、江苏省农业科学院粮食作物研究所培育的半糯粳稻品系9份 Xudao9, Ningjing 8, Suxiangjing 100, Nanjing 46, Nanjing 5055, Nanjing 9108, Nanjing 2728, Nanjing 505, Nanjing 3908, Nanjing 5718, Nanjing 58, Nanjing jinggu, 9 breeding lines of semi-waxy japonica rice bred by the Institute of Food Crops, Jiangsu Academy of Agricultural Sciences

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参考文献 34

[1]	崔晶, 楠谷彰人, 松江勇次, 森田茂纪 . 中日合作水稻品质·食味研究的现状和展望. 北方水稻, 2011,41(4):1-6.
	Cui J, Akihito K, Yuji M, Shigenori M . Present situation and expectation of the rice quality and eating under Sino-Japanese cooperation. North Rice, 2011,41(4):1-6 (in Chinese with English abstract).
[2]	熊善柏, 赵思明, 李建林, 谭汝成 . 米饭理化指标与感官品质的相关性研究. 华中农业大学学报, 2002,21(1):83-87.
	Xiong S B, Zhao S M, Li J L, Tan R C . Study on the correlations between physicochemical properties and sensory qualities of cooked rice. J Huazhong Agric Univ, 2002,21(1):83-87 (in Chinese with English abstract).
[3]	朱满山, 汤述翥, 顾铭洪 . RVA谱在稻米蒸煮食味品质评价及遗传育种方面的研究进展. 中国农学通报, 2005,21(8):59-64.
	Zhu M S, Tang S Z, Gu M H . Progress in the study on the assessing, genetic and breeding of the rice starch RVA profile in rice eating quality. Chin Agric Sci Bull, 2005,21(8):59-64 (in Chinese with English abstract).
[4]	郑英杰, 于亚辉, 李振宇, 陈广红, 夏明, 阙补超 . 北方两系杂交粳稻淀粉RVA谱特征与食味品质的关系. 中国稻米, 2018,24(3):49-54.
	Zheng Y J, Yu Y H, Li Z Y, Chen G H, Xia M, Que B C . Relationship between RVA profile characteristics and eating value of japonica two line hybrid rice in northern China. China Rice, 2018,24(3):49-54 (in Chinese with English abstract).
[5]	张丽霞, 余飞宇, 闫影, 王冬兰, 李瑞清, 曹黎明, 万常照, 吴书俊 . 长三角地区54份粳稻种质食味指标的相关性分析. 上海农业学报, 2018,34(5):37-43.
	Zhang L X, Yu F Y, Yan Y, Wang D L, Li R Q, Cao L M, Wan C Z, Wu S J . Association analysis of eating quality traits in 54 Japonica rice germplasms in Yangtze River Delta region. Acta Agric Shanghai, 2018,34(5):37-43 (in Chinese with English abstract).
[6]	贾良, 丁雪云, 王平荣, 邓晓建 . 稻米淀粉RVA谱特征及其与理化品质性状相关性的研究. 作物学报, 2008,34:790-794.
	Jia L, Ding X Y, Wang P R, Deng X J . Rice RVA profile characteristics and correlation with the physical/chemical quality. Acta Agron Sin, 2008,34:790-794 (in Chinese with English abstract).
[7]	张昌泉, 赵冬生, 李钱峰, 顾铭洪, 刘乔泉 . 稻米品质性状基因的克隆与功能研究进展. 中国农业科学, 2016,49:4267-4283.
	Zhang C Q, Zhao D S, Li Q F, Gu M H, Liu Q Q . Progresses in research on cloning and functional analysis of key genes involving in rice grain quality. Sci Agric Sin, 2016,49:4267-4283 (in Chinese with English abstract).
[8]	朱霁晖, 张昌泉, 顾铭洪, 刘巧泉 . 水稻Wx基因的等位变异及育种利用研究进展. 中国水稻科学, 2015,29:431-438.
	Zhu J H, Zhang C Q, Gu M H, Liu Q Q . Progress in the allelic variation of Wx gene and its application in rice breeding. Chin J Rice Sci, 2015,29:431-438 (in Chinese with English abstract).
[9]	Jeon J S, Ryoo N, Hahn T R, Walia H, Nakamura Y . Starch biosynthesis in cereal endosperm. Plant Physiol Bioch, 2010,48:383-392. doi: 10.1016/j.plaphy.2010.03.006
[10]	Gao Z Y, Zeng D L, Cheng F M, Tian Z X, Guo L B, Su Y, Yan M X, Jiang H, Dong G J, Huang Y C, Han B, Li J Y, Qian Q . ALK, the key gene for gelatinization temperature is a modiﬁer gene for gel consistency in rice. J Integr Plant Biol, 2011,53:756-765. doi: 10.1111/j.1744-7909.2011.01065.x
[11]	Tian Z X, Qian Q, Liu Q Q, Yan M X, Liu X F, Yan C J, Liu G F, Gao Z Y, Tang S Z, Zeng D L, Wang Y H, Yu J M, Gu M H, Li J Y . Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities. Proc Natl Acad Sci USA, 2009,106:21760-21765.
[12]	陈温福, 潘文博, 徐正进 . 我国粳稻生产现状及发展趋势. 沈阳农业大学学报, 2006,37:801-805.
	Chen W F, Pan W B, Xu Z J . Current situation and trends in production of japonica rice in China. J Shenyang Agr Univ, 2006,37:801-805 (in Chinese with English abstract).
[13]	王小虎, 钟卫国, 李标, 潘斌清, 端木李玲, 俞良, 胡江, 端木银熙, 梁国华, 钱前 . 江苏省杂交粳稻与常规粳稻新品种主要性状比较. 江苏农业学报, 2017,33:729-738.
	Wang X H, Zhong W G, Li B, Pan B Q , Duan-Mu L L, Yu L, Hu J, Duanmu Y X, Liang G H, Qian Q. Comparative analysis of major traits of new varieties between japonica hybrid rice and conventional japonica rice in Jiangsu province. Jiangsu J Agric Sci, 2017,33:729-738 (in Chinese with English abstract).
[14]	陈培峰, 顾俊荣, 乔中英, 赵步洪, 季红娟, 董明辉 . 江苏省主要粳稻品种稻米品质研究. 西南农业学报, 2018,31:877-883.
	Chen P F, Gu J R, Qiao Z Y, Zhao B H, Ji H J, Dong M H . Study on rice quality of main japonica rice varieties in Jiangsu province. Southwest China J Agric Sci, 2018,31:877-883 (in Chinese with English abstract).
[15]	王才林, 张亚东, 朱镇, 赵凌, 陈涛, 林静 . 优质水稻新品种南粳46的选育与应用. 中国稻米, 2008, ( 3):38-40.
	Wang C L, Zhang Y D, Zhu Z, Zhao L, Chen T, Lin J . Breeding and application of a new high quality rice variety Nanjing 46. China Rice, 2008, ( 3):38-40 (in Chinese with English abstract).
[16]	王才林, 张亚东, 朱镇, 姚姝, 赵庆勇, 陈涛, 周丽慧, 赵凌 . 优良食味粳稻新品种南粳9108的选育与利用. 江苏农业科学, 2013,41(9):86-88.
	Wang C L, Zhang Y D, Zhu Z, Yao S, Zhao Q Y, Chen T, Zhou L H, Zhao L . Development of a new japonica rice variety Nanjing 9108 with good eating quality. Jiangsu Agric Sci, 2013,41(9):86-88 (in Chinese with English abstract).
[17]	王才林, 张亚东, 朱镇, 陈涛, 赵庆勇, 赵凌, 周丽慧, 姚姝 . 优良食味粳稻新品种南粳5055的选育及利用. 农业科技通讯, 2012, ( 2):84-87.
	Wang C L, Zhang Y D, Zhu Z, Chen T, Zhao Q Y, Zhao L, Zhou L H, Yao S . Breeding and application of new good eating quality rice variety Nanjing 5055. Bull Agr Sci Technol, 2012, ( 2):84-87 (in Chinese).
[18]	陈培峰, 乔中英, 谢裕林, 朱勇良 . 优质多抗香稻新品种‘苏香粳100’的选育与应用. 作物研究, 2017,31(1):15-17.
	Chen P F, Qiao Z Y, Xie Y L, Zhu Y L . Breeding and application of new high-quality, multi-resistant and fragrant rice variety ‘Suxiangjing 100’. Crop Res, 2017,31(1):15-17 (in Chinese).
[19]	赵春芳, 岳红亮, 黄双杰, 周丽慧, 赵凌, 张亚东, 陈涛, 朱镇, 赵庆勇, 姚姝, 梁文化, 路凯, 王才林 . 南粳系列品种的食味品质及理化特性. 中国农业科学, 2019,52:909-920.
	Zhao C F, Yue H L, Huang S J, Zhou L H, Zhao L, Zhang Y D, Chen T, Zhu Z, Zhao Q Y, Yao S, Liang W H, Lu K, Wang C L . Eating quality and physicochemical properties in Nanjing rice varieties. Sci Agric Sin, 2019,52:909-920 (in Chinese with English abstract).
[20]	朱盈, 徐栋, 胡蕾, 花辰, 陈志峰, 张振振, 周年兵, 刘国栋, 张洪程, 魏海燕 . 江淮优良食味高产中熟常规粳稻品种的特征. 作物学报, 2019,45:578-588.
	Zhu Y, Xu D, Hu L, Hua C, Chen Z F, Zhang Z Z, Zhou N B, Liu G D, Zhang H C, Wei H Y . Characteristics of medium- maturity conventional japonica rice with good taste and high yield in Jianghuai area. Acta Agron Sin, 2019,45:578-588 (in Chinese with English abstract).
[21]	陈涛, 骆名瑞, 张亚东, 朱镇, 赵凌, 赵庆勇, 周丽慧, 姚姝, 于新, 王才林 . 利用四引物扩增受阻突变体系PCR技术检测水稻低直链淀粉含量基因Wx-mq. 中国水稻科学, 2013,27:529-534.
	Chen T, Luo M R, Zhang Y D, Zhu Z, Zhao L, Zhao Q Y, Zhou L H, Yao S, Yu X, Wang C L . Detection of Wx-mq gene for low amylose content by tetra-primer amplification refractory mutation system PCR in rice. Chin J Rice Sci, 2013,27:529-534 (in Chinese with English abstract).
[22]	徐铨, 唐亮, 徐凡, 福嶌阳, 黄瑞冬, 陈温福, 徐正进 . 粳稻食味品质改良研究现状与展望. 作物学报, 2013,39:961-968.
	Xu Q, Tang L, Xu F, Fukushima A, Huang R D, Chen W F, Xu Z J . Research advances and prospects of eating quality improvement in japonica rice( Oryza sativa L.). Acta Agron Sin, 2013,39:961-968 (in Chinese with English abstract).
[23]	Nakamura S, Cui J, Zhang X, Yang F, Xu X M, Sheng H, Ohtsubo K . Comparison of eating quality and physicochemical properties between Japanese and Chinese rice cultivars. Biosci Biotech Bioch, 2016,80:2437-2449. doi: 10.1080/09168451.2016.1220823
[24]	Yamakawa H, Hirose T, Kuroda M, Yamaguchi T . Comprehensive expression profiling of rice grain filling-related genes under high temperature using DNA microarray. Plant Physiol, 2007,144:258-277.
[25]	查曼 . 高温影响水稻不同Wx等位基因表达及品质形成的研究. 扬州大学硕士学位论文, 江苏扬州, 2017.
	Zha M . The Research of Different Wx Alleles Expressions and Quality Formation Responding to High Temperature. MS Thesis of Yangzhou University, Yangzhou, Jiangsu, China, 2017 (in Chinese with English abstract).
[26]	王慧, 张从合, 陈金节, 严志, 周桂香, 黄艳玲 . 稻米品质性状影响因素及相关基因研究进展. 中国稻米, 2018,24(4):16-21.
	Wang H, Zhang C H, Chen J J, Yan Z, Zhou G X, Huang Y L . Research advances in the influence factors and related genes of rice quality traits. China Rice, 2018,24(4):16-21 (in Chinese with English abstract).
[27]	于新, 赵庆勇, 赵春芳, 张亚东, 朱镇, 赵凌, 陈涛, 周丽慧, 姚姝, 王才林 . 携带Wx-mq基因的不同类型水稻新品种(系)直链淀粉含量分析. 江苏农业学报, 2012,28:1218-1222.
	Yu X, Zhao Q Y, Zhao C F, Zhang Y D, Zhu Z, Zhao L, Chen T, Zhou L H, Yao S, Wang C L . Analyses of amylose contents in different types of new rice varieties (lines) carrying Wx-mq gene. Jiangsu J Agric Sci, 2012,28:1218-1222 (in Chinese with English abstract).
[28]	蔡一霞, 刘春香, 王维, 张洪熙, 张祖建, 杨静, 唐汉忠 . 灌浆期表观直链淀粉含量相似品种稻米胶稠度和RVA谱的动态差异. 中国农业科学, 2011,44:2439-2445.
	Cai Y X, Liu C X, Wang W, Zhang H X, Zhang Z J, Yang J, Tang H Z . Dynamic differences of the RVA profile and gel consistency in two rice varieties with similar apparent amylose content during grain filling. Sci Agric Sin, 2011,44:2439-2445 (in Chinese with English abstract).
[29]	胡培松, 翟虎渠, 唐邵清, 万建民 . 利用RVA 快速鉴定稻米蒸煮及食味品质的研究. 作物学报, 2004,30:519-524.
	Hu P S, Zhai H Q, Tang S Q, Wan J M . Rapid evaluation of rice cooking and palatability quality by RVA profile. Acta Agron Sin, 2004,30:519-524 (in Chinese with English abstract).
[30]	李刚, 邓其明, 李双成, 王世全, 李平 . 稻米淀粉RVA谱特征与品质性状的相关性. 中国水稻科学, 2009,23:99-102.
	Li G, Deng Q M, Li S C, Wang S Q, Li P . Correlation analysis between RVA profile characteristics and quality in rice. Chin J Rice Sci, 2009,23:99-102 (in Chinese with English abstract).
[31]	隋炯明, 李欣, 严松, 严长杰, 张蓉, 汤述翥, 陆驹飞, 陈宗祥, 顾铭洪 . 稻米淀粉RVA谱特征与品质性状相关性研究. 中国农业科学, 2005,38:657-663.
	Sui J M, Li X, Yan S, Yan C J, Zhang R, Tang S Z, Lu J F, Chen Z X, Gu M H . Studies on the rice RVA profile characteristics and its correlation with the quality. Sci Agric Sin, 2005,38:657-663 (in Chinese with English abstract).
[32]	Sata H, Yasuhiro S, Sakai M, Imbe T . Molecular characterization of Wx-mq, a novel mutant gene for low-amylose content in endosperm of rice( Oryza sativa L.). Breed Sci, 2002,52:131-135.
[33]	Yang J, Wang J, Fan F J, Zhu J Y, Chen T, Wang C L, Zheng T Q, Zhang J, Zhong W G, Xu J L . Development of AS-PCR marker based on a key mutation confirmed by resequencing of Wx-mp in Milky Princess and its application in japonica soft rice( Oryza sativa L.) breeding. Plant Breed, 2013,132:595-603.
[34]	Bao J S . Towards understanding of the genetic and molecular basis of eating and cooking quality of rice. Cereal Food World, 2012,57:148-156.

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引物名称 Primer	引物序列 Primer sequence (5°-3°)	片段长度 Fragment length (bp)
Wx-seq1	F: GTGCCACCGTCGCAGGAGAAGAGATGT	1753
	R: GGGGTGGTGGTGTGGGTGGCTATTTGT
Wx-seq2	F: GGCTTCACGCAACGGCGCTACAAATAG	1812
	R: CCATTCGCCTGCAAAGAACACAAGAAC
Wx-seq3	F: TCGTCGCTGACCGTCGTCGTCTTCAAC	2327
	R: CGCAAGCACAGGGCTGGAGAAATCAAC
Wx-seq4	F: ACGGAACGGTATACAATTTCCATCTAT	1096
	R: ACATAAATTCAGGGTCCGATAAGATAC
OsSSIIa-seq1	F: CGCGCACACACTCAAACCC	1573
	R: ATCTAACAGCTGGAGTAGCCTT
OsSSIIa-seq2	F: CAGGCCCATACAATTAGGGAT	1426
	R: TGTCCCTGCTCAATAATTACCAT
OsSSIIa-seq3	F: AGTTCAGCAATTTCTCCGTTT	1614
	R: TCACATCCATTCAGACACGAG
Wx-mp-O	F: ATGTTGTGTTCTTGTGTTCTTTGCAGGC	439/292/200
	R: GTAGATCTTCTCACCGGTCTTTCCCCAA
Wx-mp-I	F: GGGTGAGGTTTTTCCATTGCTACAATCG
	R: GTCGATGAACACACGGTCGACTCAAT

性状 Trait	江苏普通粳稻 Jiangsu common japonica rice				东北粳稻 Northeast japonica rice				江苏半糯粳稻 Jiangsu semi-waxy japonica rice
性状 Trait	均值 Mean	最小值 Min.	最大值 Max.	变异系数 CV (%)	均值 Mean	最小值 Min.	最大值 Max.	变异系数 CV (%)	均值 Mean	最小值 Min.	最大值 Max.	变异系数 CV (%)
AC (%)	16.5 b	14.2	18.9	7.8	19.0 a	14.8	22.6	9.4	9.2 c	7.5	11.0	10.3
PC (%)	9.0 a	7.2	11.4	10.7	8.5 b	6.4	10.9	12.8	9.2 a	7.1	10.6	9.5
GC (mm)	68.8 b	52.0	85.0	14.4	69.4 b	58.5	86.5	10.2	84.2 a	76.5	98.5	7.2
GT (^°C)	73.4 a	68.9	80.0	3.5	70.7 b	67.3	74.5	2.2	73.2 a	69.6	80.7	3.8
PV (cP)	2462.0 a	1973.0	2944.0	10.5	2441.5 a	1796.0	2946.0	9.5	2502.8 a	1705.0	2953.0	15.0
TV (cP)	1560.4 a	1210.0	1950.0	13.3	1586.1 a	1110.0	2104.0	15.6	1261.8 b	763.0	1765.0	22.4
FV (cP)	2602.4 a	2135.0	3017.0	8.7	2634.9 a	2015.0	3243.0	10.6	1823.0 b	1229.0	2461.0	18.3
BDV (cP)	901.6 b	586.0	1284.0	18.4	855.4 b	479.0	1222.0	22.0	1241.0 a	942.0	1683.0	16.5
SBV (cP)	140.4 a	-193.0	456.0	105.9	193.4 a	-212.0	471.0	86.5	-679.9 b	-1269.0	-334.0	36.5
Pet (min)	6.4 a	6.1	6.7	2.6	6.5 a	6.1	6.9	3.4	5.9 b	5.2	6.3	5.1

性状 Trait	直链淀粉含量AC	蛋白质含量PC	胶稠度 GC	糊化温度 GT	峰值黏度 PV	热浆黏度 TV	最终黏度 FV	崩解值 BDV	消减值 SBV
蛋白质含量PC	-0.054
	0.278
	0.061
直链淀粉含量GC	0.307	0.343^*
	-0.081	0.097
	-0.204	-0.036
糊化温度 GT	-0.291	0.662^**	-0.137
	-0.176	0.336	0.296
	-0.366	0.269	0.348
峰值黏度 PV	-0.089	0.427^*	0.268	0.693^**
	0.046	0.251	-0.047	0.455^**
	-0.056	-0.187	0.111	0.123
热浆黏度 TV	-0.228	0.433^**	0.295	0.470^**	0.768^**
	0.329	0.343	0.148	0.479^**	0.691^**
	0.240	-0.146	-0.036	-0.234	0.842^**
最终黏度 FV	-0.159	0.334^*	0.267	0.408^*	0.820^**	0.938^**
	0.285	0.389^*	0.068	0.404^*	0.803^**	0.906^**
	0.400	-0.165	-0.072	-0.323	0.761^**	0.972^**
崩解值 BDV	0.146	0.032	0.050	0.493^**	0.600^**	-0.051	0.108
	-0.375^*	-0.153	-0.252	-0.070	0.321	-0.463^**	-0.203
	-0.435^*	-0.139	0.254	0.549^**	0.667^**	0.159	0.049
消减值 SBV	-0.088	-0.239	-0.060	-0.584^**	-0.492^**	0.090	0.095	-0.880^**
	0.414^*	0.303	0.179	0.047	-0.039	0.562^**	0.565^**	-0.784^**
	0.625^**	0.057	-0.265	-0.622^**	-0.487^*	0.036	0.196	-0.942^**
峰值时间 Pet	-0.162	0.311	0.223	-0.003	0.129	0.678^**	0.526^**	-0.647^**	0.576^**
	0.310	0.320	0.284	0.522^**	0.358^*	0.885^**	0.678^**	-0.721^**	0.642^**
	0.551^**	0.045	-0.305	-0.635^**	0.157	0.645^**	0.708^**	-0.604^**	0.717^**

江苏和东北粳稻稻米理化特性及Wx和OsSSIIa基因序列分析

Physicochemical properties and sequence analysis of Wx and OsSSIIa genes in japonica rice cultivars from Jiangsu province and northeast of China

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