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作物学报 ›› 2009, Vol. 35 ›› Issue (5): 848-854.doi: 10.3724/SP.J.1006.2009.00848

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

郑麦9023春化基因VRN-1的组成及表达

袁秀云1,2，李永春1,**，孟凡荣3，闫延涛1，尹钧1,*   

  1. 1河南农业大学国家小麦工程技术研究中心，河南郑州450002；2郑州师范高等专科学校，河南郑州450044；3河南农业大学生命科学学院，河南郑州450002
  • 收稿日期:2008-09-11 修回日期:2009-02-14 出版日期:2009-05-12 网络出版日期:2009-03-23
  • 通讯作者: 尹均
  • 基金资助:

    本研究由国家自然科学基金（30671261），国家“十一五”科技支撑计划重大项目（2006BAD02A07-4)资助。

Allelic Composition and Expression of  Vernalization Gene Vrn-1 in Wheat Cultivar Zhengmai 9023

YUAN Xiu-Yun12，LI Yong-Chun1**，MENG Fan-Rong3，YAN Yan-Tao1,YIN Jun1*   

  1. 1National Engineering Research Center for Wheat,Henan Agricultural University,Zhengzhou 450002,China;2Zhengzhou Normal College,Zhengzhou 450044,China;3College of Life Science,Henan Agricultural University,Zhengzhou 450002,China
  • Received:2008-09-11 Revised:2009-02-14 Published:2009-05-12 Published online:2009-03-23
  • Contact: YIN Jun

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1510

被引次数

3

摘要：

以郑麦9023叶片为材料，利用序列特异性PCR扩增技术克隆了春化基因VRN-1，并通过0~2℃冰箱模拟春化处理0、10、20和30 d，对该基因在一叶期至九叶期叶片中的表达进行了分析。PCR分析表明，VRN-1基因在郑麦9023的A和D基因组中均为隐性，在B基因组中为显性，基因等位类型为vrnA1VrnB1vrnD1。在克隆VRN-A1、VRN-B1和VRN-D1基因序列的基础上，设计了3个等位基因的特异引物，并利用该特异引物进行半定量RT-PCR分析。结果显示，在未经春化处理的条件下，一叶期VRN-A1和VRN-D1均未检测到表达，而VRN-B1已有较低水平的表达；从三叶期开始，3个等位基因都有较高水平的表达，并一直持续至开花期。在春化处理10、20和30 d条件下，VRN-1的3个等位基因在一叶期就出现较高水平的表达，并保持至开花期。

关键词: 郑麦9023, 春化基因vrn-1, 基因克隆, 半定量RT-PCR

Abstract:

Zhengmai 9023 is an elite winter wheat (Triticum aestivum L.) cultivar grown in a large scale in China, and often injured by coldness when it easily starts reproductive growth before winter because of its weak vernalization characteristic. Vernalization gene VRN-1 is one of the key genes controlling the conversion from vegetative growth to reproductive growth in wheat. To explore the regulation mechanism of vernalization in Zhengmai 9023, the VRN-1 gene was cloned from leaf tissues using gene-specific PCR amplification technique, and its expressions were analyzed under simulated vernalization at 0–2°C for 0, 10, 20, and 30 d. The gene-specific primers were designed for semiquantitative PCR analysis based on the sequences of the VRN-A1, VRN-B1, and VRN-D1, which were cloned from Zhengmai 9023. The results showed that the genotype of VRN-1 was vrnA1VrnB1vrnD1 with the unique dominant allele in B genome of Zhengmai 9023. Under the treatment of 0 d vernalization, the expressions of VRN-A1 and VRN-D1 were not detected at one-leaf stage, whereas VRN-B1 expressed at a low level and the expressions of the three VRN-1 alleles were all at relative high levels from three-leaf stage to flowering stage. However, under the treatments with 10 to 30 d vernalizaion, the three alleles of VRN-1 gene showed high-level expressions throughout the period from one-leaf to flowering stages.

Key words: Zhengmai 9023, Vernalization gene VRN-1;Gene cloning, Semiquantitative RT-PCR


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