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作物学报 ›› 2009, Vol. 35 ›› Issue (8): 1386-1394.doi: 10.3724/SP.J.1006.2009.01386

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用双向导入系解析水稻抽穗期和株高QTL及其与环境互作表达的遗传背景效应

王韵1,2,程立锐2,孙勇2,周政2,朱苓华2,徐正进1,徐建龙2,*,黎志康2,3   

  1. 1沈阳农业大学/农业部作物生理生态遗传育种重点开放实验室，辽宁沈阳110161；2中国农业科学院作物科学研究所/农作物基因资源与遗传改良国家重点科学工程，北京100081；3International Rice Research Institute,DAPO Box 7777,Metro Manila,Philippines
  • 收稿日期:2009-01-21 修回日期:2009-03-13 出版日期:2009-08-12 网络出版日期:2009-06-10
  • 通讯作者: 徐建龙, E-mail: xujl@caas.net.cn
  • 基金资助:

    本研究由引进国际先进农业科学技术计划（948计划）项目（2006-G51和2006-G1）资助。

Genetic Background Effect on QTL Expression of Heading Date and Plant Height and Their Interaction with Environment in Reciprocal Introgression Lines of Rice 

WANG Yun1,2, CHENG Li-Rui2, SUN Yong2, ZHOU Zheng2, ZHU Ling-Hua2, XU Zheng-Jin1, XU Jian-Long2,*,LI Zhi-Kang2,3   

  1. 1 Shenyang Agricultural University / Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang 110161, China;
    2 Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines
  • Received:2009-01-21 Revised:2009-03-13 Published:2009-08-12 Published online:2009-06-10
  • Contact: XU Jian-Long, E-mail: xujl@caas.net.cn

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被引次数

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摘要：

利用粳稻Lemont和籼稻特青相互导入构建的遗传背景基本一致的双向回交导入系群体，分别在北京和海南环境定位影响抽穗期和株高的主效QTL及其环境互作，分析QTL及其与环境互作表达的遗传背景效应。在北京和海南分别检测到影响抽穗期和株高的主效QTL 16个和17个，其中有5个主效QTL (QHd2、QHd8a、QPh3、QPh5和QPh12)在两种背景下同时被检测到，表明多数主效QTL的表达具有遗传背景特异性。两种背景下检测到影响抽穗期的3个主效QTL (QHd8a、QHd9和QHd10b)存在环境互作，其中QHd8a与海南环境的互作在两种背景下提早抽穗2~3 d，与北京环境的互作则延迟抽穗2~3 d，是影响抽穗期的一个重要主效QTL。通过与以往相同亲本来源的7个不同定位群体在不同环境下定位结果的比较，鉴定出一些在不同遗传背景和环境下稳定表达的主效QTL，如QHd3、QHd8a、QPh3和QPh4，适宜用于水稻抽穗期和株高的分子标记改良。基于QTL定位结果，本文对如何通过分子标记辅助改良品种在不同环境下的抽穗期进行了深入探讨。

关键词: 数量性状基因座, 遗传背景, 基因与环境互作, 双向导入系, 抽穗期, 株高

Abstract:

Expression of quantitative trait is affected by genetic background and environment. Genetic background effect on QTL mapping and QTL by environment interaction for heading date (HD) and plant height (PH) in Beijing and Hainan environments were dissected using a large set of reciprocal introgression lines (ILs) derived from a japonica variety “Lemont” and indica variety “Teqing”. The two sets of ILs showed transgressive segregation for the two traits. Total 16 and 17 main-effect QTLs were identified for HD and PH in the two environments, respectively. Among them, only five main-effect QTLs (QHd2, QHd8a, QPh3, QPh5,and QPh12) were detected under the two backgrounds, indicating expression of most main-effect QTLs are specific to genetic background. Three main-effect QTLs (QHd8a, QHd9, and QHd10b) by environment interactions for HD were significantly detected under the two backgrounds, of which that of QHd8a had earlier heading for 2–3 days in Hainan, but delayed heading for 2–3 days in Beijing. Therefore, QHd8a could be considered as an important main-effect QTL for HD. By comparison with the QTL mapping results previously identified in the seven different mapping populations derived from the same parents in different environments, some stably expressed main-effect QTLs including QHd3, QHd8a, QPh3,and QPh4 were identified under different backgrounds and environments, suggesting these QTLs could be used in marker-assisted breeding for HD and PH. On the basis of the mapping information, marker-assisted improvement of HD for a rice variety under different environments was deeply discussed.

Key words: Quantitative trait loci, Genetic background, Gene X environment interaction, Reciprocal introgression lines, Heading date, Plant height

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