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作物学报 ›› 2010, Vol. 36 ›› Issue (3): 391-400.doi: 10.3724/SP.J.1006.2010.00391

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

云南茶树资源遗传多样性与亲缘关系的ISSR分析

刘本英1, 2，李友勇2，唐一春2，王丽鸳1，成浩1,*，王平盛2   

  1. 1中国农业科学院茶叶研究所茶树资源与改良研究中心/国家茶树改良中心，浙江杭州310008；2云南省农业科学院茶叶研究所，云南勐海666201
  • 收稿日期:2009-09-08 修回日期:2009-12-08 出版日期:2010-03-12 网络出版日期:2010-01-22
  • 通讯作者: 成浩, E-mail: chenghao@mail.tricaas.com
  • 基金资助:

    本研究由国家科技基础条件平台项目建设计划（2005DKA21002）和浙江省重点科技计划项目（2006C22070）资助。

Genetic Diversity and Relationship of Tea Germplasm in Yunnan Revealed by ISSR Analysis

LIU Ben-Ying1,2,LI You-Yong2,TANG Yi-Chun2,WANG Li-Yuan1,CHENG Hao1,*,WANG Ping-Sheng2   

  1. 1 Research Center for Tea Germplasm and Improvement, Tea Research Institute of Chinese Academy of Agricultural Science, National Center for Tea Improvement, Hangzhou 310008, China; 2 Tea Research Institute of Yunnan Academy of Agricultural Science, Menghai 666201, China
  • Received:2009-09-08 Revised:2009-12-08 Published:2010-03-12 Published online:2010-01-22
  • Contact: CHENG Hao, E-mail: chenghao@mail.tricaas.com

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2051

被引次数

66

摘要：

以8个种群134份云南茶树资源为材料，应用ISSR标记方法，进行了遗传多样性与亲缘关系分析。结果表明，18个多态性ISSR引物对全部试验材料进行PCR扩增，共获得475条稳定的谱带，其中多态性谱带470条(占98.9%)，遗传多样性丰富；应用Nei-Li相似系数法估算了134个材料间的相似系数为0.445~0.819，平均为0.512，说明茶树资源间的遗传基础较宽；对134份茶树资源的分子系统聚类分析(UPGMA)将资源分为3大组，聚类结果与地理距离没有明显的相关性；主成分分析(PCA)表明主成分分析的结果与系统聚类基本一致，但是主成分分析更加直观清晰地显示各个材料间的亲缘关系；大厂茶等8个种群间的遗传相似系数于0.850~0.987间，平均为0.92，表明不同种群间的遗传差异较小。

关键词: 云南, 茶树资源, ISSR, 遗传多样性, 亲缘关系

Abstract:

Tea is an important beverage crop in the world. Yunnan region in China is the origin center of tea plants. Our knowledge on genetic diversity and relationship of tea germplasm in Yunnan province is critical to guide tea breeding. In order to provide theoretical information for using tea germplasm in tea breeding, we investigated the relationship and genetic diversity of the tea germplasm in Yunnan province. Eight species, including 134 tea varieties were used to detect the genetic variation by inter-simple sequence repeats (ISSR) analysis. The result showed that 475 DNA fragments among all 134 tea accessions were amplified, using 18 reliable ISSR primers, among which 470 DNA bands were polymorphic (PPB=98.9%). This indicated that a great amount of genetic polymorphism exists among tea germplasm tested. The genetic similarity (GS) among the tested genotypes ranged from 0.445 to 0.819, with an average of 0.512, indicating a wide gene pool among tea varieties in Yunnan. The cluster analysis presented that these resources were divided into three main groups using the unweighted pair-group method with arithmetic average (UPGMA) based on ISSR molecular marker data, but the dendrogram did not indicate clear division among tested varieties based on their geographical origin. Principal Component analysis (PCA) for ISSR data showed that PCA supported UPGMA clustering result, but showed more explicit relationships among the test accessions with different lays, orientationsand positions. The GS among 8 populations ranged from 0.850 to 0.987, with an average of 0.92, indicating that there existed a small variation of genetic diversity among different population. The findings of this research would be favorable for the further practice, such as tea breeding, the molecular genetic linkage mapping and the DNA fingerprint building of tea germplasm.

Key words: Yunnan, Tea Germplasm, ISSR, Genetic diversity, Genetic Relationship


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