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作物学报 ›› 2010, Vol. 36 ›› Issue (3): 385-390.doi: 10.3724/SP.J.1006.2010.00385

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

黄淮冬麦区小麦冬、春性改良及分子标记辅助选择技术初探

张晶1，吴锁伟1，刘秉华1，宋梅芳2，周朋1，郭春燕3，詹克慧3，王山荭1，杨丽1，董冬1，于立强4，李辉利4，周阳1*，杨建平1*   

  1. 1中国农业科学院作物科学研究所/农作物基因资源与基因改良重大科学工程，北京100081；2山东农业科学院蔬菜研究所，山东济南250100；3河南农业大学农学院，河南郑州450002；4河北省赵县农业科学研究所，河北石家庄051530
  • 收稿日期:2009-10-09 修回日期:2010-01-08 出版日期:2010-03-12 网络出版日期:2010-01-22
  • 通讯作者: 周阳, E-mail: zhouyang@mail.caas.net.cn, Tel: 010-82108628; 杨建平, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859
  • 基金资助:

    本研究由国家高科技研究发展计划（863计划）项目（2006AA10Z1F2）和国家重点基础研究发展计划（973计划）项目（2009CB118300）资助。

Genetic Improvement of Wheat Growth Habit and Its Molecular Marker-Assisted Selection in Yellow and Huai River Reaches

ZHANG Jing1, WU Suo-Wei1, LIU Bing-Hua1, SONG Mei-Fang2, ZHOU Peng1, GUO Chun-Yan3, ZHAN Ke-Hui3, WANG Shan-Hong1, YANG Li1, DONG Dong1, YU Li-Qiang4, LI Hui-Li4, ZHOU Yang1,*, and YANG Jian-Ping1,*   

  1. 1Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Vegetable Research Institute,Shandong Academy of Agricultural sciences,Jinan 250100,China;3College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China;4Zhaoxian Agricultural Research Institute of Hebei Province,Shijiazhuang 051530.China
  • Received:2009-10-09 Revised:2010-01-08 Published:2010-03-12 Published online:2010-01-22
  • Contact: ZHOU Yang,E-mail: zhouyang@mail.caas.net.cn, Tel: 010-82108628; YANG Jian-ping, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859

PDF

1755

被引次数

7

摘要：

通过对小麦品种石麦12春化特性的遗传和分子标记研究，探索黄淮冬麦区小麦冬、春性改良途径和分子标记辅助选择技术。石麦12与冬性品种石家庄8号杂交后代F2:3株系中的春性株系、冬春性分离株系、冬性株系的分离比例符合1∶2∶1，表明石麦12具有一个显性春化基因，经已知春化基因的基因特异性标记鉴定为Vrn-D1。利用Vrn-D1的基因特异性标记对上述F2:3株系进行冬、春性鉴定的结果与表型鉴定结果一致，说明该分子标记可用于小麦冬、春性改良中对Vrn-D1的辅助选择。在高海拔、长日照地区夏播是小麦冬、春性表型鉴定的一个快速、简便途径。

关键词: 小麦育种, 春化基因, 分子标记

Abstract:

The vernalizatioin gene in wheat cultivar Shimai 12 (Triticum aestivum L.) was investigated to find a more efficient way for the improvement of growth habit of wheat cultivars grown in Yellow and Huai River Reaches. In the F2:3 lines derived from the cross between Shimai 12 and Shijiazhuang 8 (a winter wheat cultivar), the ratio of the spring, segregating, and winter lines was in accordance to 1:2:1 based on chi-square test, showing that the spring growth habit of Shimai 12 was determined by single dominant gene, which was identified as Vrn-D1 by gene-specific markers. The Vrn-D1 gene-specific marker was further used to genotype the F2:3 lines, and the results were consistent with phenotyping, verifying the available utilization of the marker assisted selection for Vrn-D1 in breeding programs. Besides, summer sowing in high altitude and cool area proved to be a cost-saving and efficient method for wheat growth habit identification.

Key words: Wheat breeding, Vernalization gene, Molecular marker

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