作物学报 ›› 2010, Vol. 36 ›› Issue (10): 1683-1690.doi: 10.3724/SP.J.1006.2010.01683
周琳璘1,宋国琦1,李红燕1,胡银岗1,2,3,*,何蓓如1
ZHOU Lin-Lin1,SONG Guo-Qi1,LI Hong-Yan1,HU Yin-Gang1,2,3,*,HE Bei-Ru1
摘要: 为揭示YS型小麦温敏雄性不育育性转换的基础, 以该类型不育系A3017不育幼穗和可育幼穗为材料构建正、反杂交SSH-cDNA文库, 从可育文库中筛选出一个与G3BP (Ras-GTPase activating protein SH3 domain-binding protein)基因同源的EST序列(GenBank登录号为DY543200)。以该EST序列的同源性比对和拼接结果为依据设计引物,在可育幼穗中扩增出一条1 230 bp的cDNA序列。该片段含有与G3BP相似的由409个氨基酸组成的结构域, 与水稻G3BP的氨基酸序列同源性为79%, 被命名为TaG3BP (GenBank登录号为GU475149)。利用Real-time PCR检测TaG3BP基因在YS型小麦温敏雄性不育系花药发育各时期中的表达模式, 发现该基因在育性转换的关键时期上调表达, 且可育条件下的表达量高于同时期不育条件下的表达量。进一步对TaG3BP在3种不同类型的小麦K型雄性不育材料的不育系及其保持系的幼穗中的表达模式进行半定量RT-PCR分析, 结果该基因在保持系幼穗中表达量较高, 在不育系中表达量较低。表明该基因可能在其育性转换中具有重要作用。
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