同源四倍体水稻花粉母细胞减数分裂期间微管骨架组织和结构变化

doi:10.3724/SP.J.1006.2010.01777

摘要/Abstract

摘要： 应用间接免疫荧光标记-激光扫描共聚焦显微术，观察了同源四倍体水稻花粉母细胞减数分裂过程的微管骨架和染色体行为变化。结果表明：同源四倍体水稻花粉母细胞微管组织形态变化与二倍体水稻的基本一致，但出现一些不同，如核周微管较长，纺锤体微管数量较多；并呈现许多异常现象。前期I细线期，微管数量少，分布不均匀；偶线期，呈现不规则网络状结构，未见明显的极性分布；粗线期，核仁解体，呈现混乱的网络状结构；双线期，微管提早解聚，不形成环绕状微管；终变期，微管数量稀少，核周未见明显的微管聚集；中期I纺锤体形态出现多种异常现象；末期I成膜体形状异常，出现点状微管；末期II-四分体时期，二分体细胞两端不分开；出现三分体细胞，其内微管混乱。以上各时期的染色体行为也出现不同程度的异常。综合认为，微管组织的异常可能与染色体的行为异常存在一定的关联，两者共同作用影响花粉发育，导致育性偏低。

关键词: 水稻(Oryza sativa L.), 同源四倍体水稻, 减数分裂, 分粉, 微管骨架

Abstract: Changes of microtubule distribution and chromosome behavior in autotetraploid rice during meiosis of PMC were detected in detail by using indirect-immunofluorescence laser scanning confocal microscopy. The result showed that microtubule distribution pattern in PMC of autotetraploid rice was similar to that of diploid rice during meiosis. But in comparison to diploid rice, different microtubule behaviors, such as longer circumferential microtubules, more spindle microtubules, were found in autotetraploid rice. At prophase I, microtubules were decreased a lot in number and distributed unevenly at leptotene. At zygotene, microtubules with no obvious polar distribution formed a diffuse network. At pachytene, nucleolus disintegration and distorted microtubules structure were found. At diplotene, no circumferential microtubule was found and microtubules depolymerization displayed in advance. At diakinesis, microtubules were decreased a lot in number. Perinuclear microtubules abnormally knitted. At MetaphaseI, many kinds of abnormalities appeared in spindle. At telophase I, phragmoplast was abnormal, and speckle microtubules were found in the cell. At telophaseII-tetra stage, two daughter cells connected, and microtubules randomly arranged in tri-fission. The results showed that microtubule abnormal reorganization pattern had a close relationship with abnormal chromosome behavior, which may act synergistically on pollen fertility and seed setting rate in autotetraploid rice.

Key words: Rice(Oryza sativa L.), Autotetraploid rice, Meiosis, Meiosis, Pollen, Microtubule

何金华, 程杏安, 陈志雄, 郭海滨, 刘向东, 卢永根. 同源四倍体水稻花粉母细胞减数分裂期间微管骨架组织和结构变化[J]. 作物学报, 2010, 36(10): 1777-1785.

HE Jin-Hua, CHENG Xing-An, CHEN Zhi-Xiong, GUO Hai-Bin, LIU Xiang-Dong, LU Yong-Gen. Changes in the Pattern of Organization of Microtubules during Meiosis in Pollen Mother Cell (PMC) of Autotetraploid Rice[J]. Acta Agron Sin, 2010, 36(10): 1777-1785.

参考文献

[1]Bingham E T, Groose R W, Woodfield D R, Kidwell K K. Complementary gene interactions in alfalfa is greater in autotetraploids than in diploids. Crop Sci, 1994, 34: 823-829
[2]Chen Z-Y(陈志勇), Wu D-Y(吴德瑜), Song W-C(宋文昌), Zhang Y-H(张玉华), Qin R-Z(秦瑞珍), Bao W-K(鲍文奎). Research advances in autotetraploid rice breeding. Sci Agric Sin (中国农业科学), 1987, 20(1): 20-24 (in Chinese with English abstract)
[3]Song W-C(宋文昌), Zhang Y-H(张玉华). Rice tetraploid and its affect on agronomic traits and nutritional constituents. Acta Agron Sin (作物学报), 1992, 18(2): 137-144 (in Chinese with English abstract)
[4]Zhang H-H(张华华), Feng J-H(冯九焕), Lu Y-G(卢永根), Yang B-Y(杨秉耀), Liu X-D(刘向东). Observation on the formation and development of autotetraploid rice embryo sac using laser scanning confocal microscopy. J Chin Electron Microscopy Soc (电子显微学报), 2003, 2(5): 380-384 (in Chinese with English abstract)
[5]Guo H-B(郭海滨), Lu Y-G(卢永根), Liu X-D(刘向东), Feng J-H(冯九焕), Yang B-Y(杨秉耀). Further observation on the formation and development of autotetraploid rice embryo sac using laser scanning confocal microscopy. Acta Laser Biol Sin (激光生物学报), 2006, 15(2): 111-117 (in Chinese with English abstract)
[6]Xiao B(肖波), Liu X-D(刘向东), Lu Y-G(卢永根), Li J-Q(李金泉), Zhao X-J(赵杏娟). Structure of embryo sac and fertilization in intersubspecific hybrids of autotetraploid rice. Acta Agron Sin (作物学报), 2005, 31(9): 1150-1156 (in Chinese with English abstract)
[7]Zhao M-H(赵明辉), Liu X-D(刘向东), Lu Y-G(卢永根), Li J-Q(李金泉), Guo H-B(郭海滨). Chromosome pairing behavior and reproduction in the hybrid developed by the interaction of different pollen sterile genes in autotetrapioid rice. Acta Agron Sin (作物学报), 2006, 32(10): 1472-1478 (in Chinese with English abstract)
[8]Hu C Y, Zeng Y X, Lu Y G, Li J Q, Liu X D. High embryo sac fertility and diversity of abnormal embryo sacs detected in autotetraploid indica/japonica hybrids in rice by whole-mount eosin B-staining confocal laser scanning microscopy. Plant Breed, 2009, 128: 187-192
[9]Long W-B(龙文波), Luan L(栾丽), Wang X(王兴), Liu Y-H(刘玉花), Tu S-B(涂升斌), Kong F-L(孔繁伦), He T(何涛). Cytogenetical comparison of restorers TP-4 and D Minghui 63 and maintainer D46B of autotetraploid rice. Hereditas (遗传), 2007, 29(4): 462-470 (in Chinese with English abstract)
[10]Luan L, Tu S B, Long W B, Wang X, Liu Y H, Kong F L, He T, Yan W G, Yu M Q. Cytogenetic studies on two F1 hybrids of autotetraploid rice varieties showing extremely high level of heterosis. Plant System Evol, 2007, 26: 205-213
[11]Schmit A C, Endle M C, Lambert A M. The perinuclear microtubule-organizing center and the synaptonemal complex of higher plants share a common antigen: its putative transfer and role in meiotic chromosome ordering. Chromosoma, 1996, 104: 405-413
[12]Xu S X, Ye X L. Changes in the pattern of organization of microtubules during microspore formation in rice (Oryza sativa L.). Acta Bot Sin, 1998, 40: 585-590
[13]Ye X-L(叶秀麟), Yeung E(杨子德), Xu S-X(徐是雄), Liang C-Y(梁承邺). Microtubule structure and male sterility in a gene-cytoplasmic male sterile line of rice, Zhenshan 97A. Acta Bot Sin (植物学报), 2003, 45(2): 183-192 (in English with Chinese abstract)
[14]Liu X D, Lu Y G, Zhu H L, Feng J H, Xu S X. Abnormal behavior of nuclei and microtubule(MT) organizational changes during embryo sac development in the poly-egg mutant, APIV of rice. Acta Bot Sin, 2004, 46: 829-838
[15]Meerow A W, Dehgan B. Pollen morphology of the eucharidead (Amaryllidaceae). Am J Bot, 1988, 75: 1857-1870
[16]Traas J A, Burgain S, Dumas R. The organization of cytoskeleton during meiosis in eggplant (Solanum melongena L.): microtubules and F-actin are both necessary for coordinated meiotic division. J Cell Sci, 1989, 92: 541-550
[17]Hause G, Hause B, Lammeren A A M. Microtubular and actin filament configurations during microspore and pollen development in Brassica napus cv. Topas. Can J Bot, 1992, 70: 1369-1376
[18]Brown R C, Lemmon B E. Pollen development in orchids 4. Cytoskeleton and ultrastructure of the unequal pollen mitosis in Phalaenopsis. Protoplasma, 1992, 167: 183-192
[19]Brown R C, Lemmon B E. Division polarity, development and configuration of microtubule arrays in Bryophyte meiosis: II. Anaphase to the tetrad. Protoplasma, 1987, 138: 1-10
[20]Uehare K, Murakami S. Arrangement of microtubules during spore formation in Equisetum arvense (Equisetaceae). Am J Bot, 1995, 82: 75-80
[21]Nick P. Plant Microtubules: Potential for Biotechnology. German: Springer, 2000. pp 159-162
[22]Dickson H G, Sheldon J M. A radial system of microtubules extending between the nuclear envelope and the plasma membrane during early male haplophase in flowering plants. Planta, 1984, 161: 86-90
[23]Feng J-H(冯九焕), Liu X-D(刘向东), Lu Y-G(卢永根). A preliminary study on microtubular cytoskeleton during microsporogenesis of rice. J South China Agric Univ (华南农业大学学报), 1999, 20(3): 1-5 (in Chinese with English abstract)
[24]Zee S Y, Ye X L. Microtubule reorganization during pollen development of rice (Oryza sativa L.). Protoplasma, 2000, 210: 188-201
[25]Xu S X, Liu X D, Feng J H, Lu Y G. Comparative studies on the changes of microtubule distribution and reorganization during the meiotic stages of development in normal (IR36) and a temperature/photoperiod sensitive male sterile line (Pei’ai 64S) of rice (Oryza sativa L.). Acta Bot Sin, 2001, 43: 221-226
[26]Zhu Z(朱徵), Xu S-X(徐是雄). Plant Cell Cytoskeleton (植物细胞骨架). Beijing: Science Press, 1996. pp 60-61 (in Chinese)
[27]Liu Z-X(刘宗贤), Qin R-Z(秦瑞珍). Selection of primary trisomics from anther culture of autotetraploid rice (Oryza sativa L.). Acta Bot Sin (植物学报), 1995, 37(2): 125-133 (in Chinese with English abstract)
[28]Liu Z-X(刘宗贤), Qin R-Z(秦瑞珍). Cytological basis of selecting primary trisomics through anther culture of tetraploid rice (Oryza sativa L.). Sci Agric Sin (中国农业科学), 1995, 28(6): 1-8 (in Chinese with English abstract)
[29]Yang M, Ma H. Male meiotic spindle lengths in normal and mutant Arabidopsis cell. Plant Physiol, 2001, 126: 622-630
[30]Higuchi T, Uhlmann F. Stabilization of microtubule dynamics at anaphase onset promotes chromosome segregation. Nature, 2005, 433: 171-176
[31]Zhang X-J(张晓菁), Chen J-S(陈劲松), Lei C(雷春), Chen Z-L(陈龙正), Qian C-T(钱春桃), Diao W-P(刁卫平). AFLP analysis of genetic differences among cucumber materials with different ploidies. Acta Bot Boreal-Occident Sin (西北植物学报), 2006, 26(11): 2265-2269 (in Chinese with English abstract)
[32]Xie H-B(谢慧波), Huang Q-C(黄群策), Li G-P(李国平), Wang X-L(汪杏莉), Ye C-Y(叶丛云), Qin G-Y(秦广雍). Differential expression of the proteins in endosperms of rice with different chromosome sets. Hereditas (遗传), 2007, 29(3): 360-364 (in Chinese with English abstract)
[33]Jiang J-Z(姜金仲), Li-Y(李云), Cheng J-X(程金新). Variation of both DNA genetic structure and reproduction traits of plant autotetraploid. Hereditas (遗传), 2006, 28(9): 1185-1190 (in Chinese with English abstract)

相关文章 15

[1]	殷家明,钟荣棋,林呐,唐章林,李加纳. 诸葛菜小孢子培养及其单倍体减数分裂染色体配对观察[J]. 作物学报, 2020, 46(02): 194-203.
[2]	郭国强，孙学武，孙平勇，尹建英，何强，袁定阳，邓华凤，袁隆平. 水稻斑马叶突变体zebra1349的表型鉴定及基因精细定位[J]. 作物学报, 2016, 42(07): 957-965.
[3]	杨波,夏敏,张孝波,王晓雯,朱小燕,何沛龙,何光华,桑贤春. 水稻早衰突变体esl6*的鉴定与基因定位[J]. 作物学报, 2016, 42(07): 976-983.
[4]	冯萍,邢亚迪,刘松,郭爽,朱美丹,娄启金,桑贤春,何光华,王楠. 水稻卷叶突变体rl28的特性与基因定位[J]. 作物学报, 2015, 41(08): 1164-1171.
[5]	张天泉,郭爽,邢亚迪,杜丹,桑贤春,凌英华,何光华. 水稻新黄绿叶基因YGL9的分子定位[J]. 作物学报, 2015, 41(07): 989-997.
[6]	陈玉宇,朱玉君,张宏伟,王琳琳,樊叶杨,庄杰云. 水稻第1染色体长臂上微效千粒重QTL qTGW1.2的验证与分解[J]. 作物学报, 2014, 40(05): 761-768.
[7]	马娇，任德勇，吴国超，朱小燕，马玲，桑贤春，凌英华，何光华. 水稻叶缘白化突变体mal的遗传分析与基因定位[J]. 作物学报, 2014, 40(04): 591-599.
[8]	李宏岩,王思哲,SHAHID Muhammad Qasim*,陈志雄,王兰,陈凤宜,刘向东,卢永根*. 从携带S₅ⁿ基因的水稻种质中发掘S_aⁿ、S_bⁿ和S_cⁿ基因[J]. 作物学报, 2013, 39(08): 1366-1376.
[9]	朱小燕,徐芳芳,桑贤春,蒋钰东,代高猛,王楠,张长伟,何光华*. 水稻叶脉白化突变体wpsm的遗传分析与基因定位[J]. 作物学报, 2013, 39(08): 1409-1415.
[10]	陈红霖,向阳海,赵纪莹,尹德东,梁国华,翟文学,江光怀. 水稻类病变突变体c5的遗传分析与目标基因的精细定位[J]. 作物学报, 2013, 39(07): 1148-1154.
[11]	代高猛,朱小燕,李云峰,凌英华,赵芳明,杨正林,何光华. 水稻类病斑突变体spl31的遗传分析与基因定位[J]. 作物学报, 2013, 39(07): 1223-1230.
[12]	程欣,任德勇,马娇,朱晓燕,桑贤春,凌英华,赵芳明,何光华. 水稻叶片灰白转黄突变体pyr1*的鉴定与基因定位[J]. 作物学报, 2013, 39(06): 992-998.
[13]	徐芳芳，桑贤春，任德勇，唐彦强，胡宏伟，杨正林，赵芳明，何光华. 水稻早衰突变体esl2的遗传分析和基因定位[J]. 作物学报, 2012, 38(08): 1347-1353.
[14]	田晓庆, 桑贤春, 赵芳明, 李云峰, 凌英华, 杨正林, 何光华. 水稻卷叶基因RL₁₃的遗传分析和分子定位[J]. 作物学报, 2012, 38(03): 423-428.
[15]	丁沃娜, 黄炜, 宁永强, 朱世华. 一个新的水稻短根毛突变体的遗传分析和基因定位[J]. 作物学报, 2012, 38(02): 240-244.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed