作物学报 ›› 2013, Vol. 39 ›› Issue (06): 992-998.doi: 10.3724/SP.J.1006.2013.00992
程欣,任德勇,马娇,朱晓燕,桑贤春,凌英华,赵芳明,何光华*
CHENG Xin,REN De-Yong,MA Jiao,ZHU Xiao-Yan,SANG Xian-Chun,LING Ying-Hua,ZHAO Fang-Ming,HE Guang-Hua*
摘要:
鉴定和克隆叶色突变基因对于深入了解叶绿素合成、降解途径的关系以及植物的光合作用有着重要的作用。从EMS诱变恢复系缙恢10号后代中鉴定出1个灰白转黄突变体pyr1,该突变体在苗期部分死亡,整张叶片呈现灰白色,在不同的生育时期叶片呈现不同的颜色,直到孕穗期叶片上部和叶缘表现黄色。苗期到抽穗期突变体叶绿素含量比野生型显著或极显著降低。透射电镜观察表明,突变体与野生型细胞结构无明显差异,但叶绿体发育异常,内部大量降解,基质片层退化。遗传分析表明该性状受1对隐性基因控制,利用326株F2隐性定位群体将PYR1基因定位在第1染色体长臂上,位于标记RM11722和Ind1之间,物理距离约92 kb,本研究为PYR1基因的图位克隆奠定了基础。
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