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作物学报 ›› 2010, Vol. 36 ›› Issue (11): 1853-1863.doi: 10.3724/SP.J.1006.2010.01853

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

基于rDNA-ITS序列探讨甘蔗近缘属种的系统进化关系

刘新龙1,苏火生1,马丽1,陆鑫1,应雄美1,蔡青1,2,范源洪1,*   

  1. 1 云南省农业科学院甘蔗研究所 / 云南省甘蔗遗传改良重点实验室, 云南开远 661600; 2 云南省农业科学院生物技术与种质资源研究所, 云南昆明 650223
  • 收稿日期:2010-03-16 修回日期:2010-06-27 出版日期:2010-11-12 网络出版日期:2010-08-30
  • 通讯作者: 范源洪, E-mail: fyhysri@vip.sohu.com
  • 基金资助:

    本研究由云南省应用基础研究计划重点项目(2006C0013Z)和国家科技基础条件平台工作项目子专题(2007DKA21002-11)资助。

Phylogenetic Relationships of Sugarcane Related Genera and Species Based on ITS Sequences of Nuclear Ribosomal DNA

LIU Xin-Long1,SU Huo-Sheng1,MA Li1,LU Xin1,YING Xiong-Mei1,CAI Qing1,2,FAN Yuan-Hong1,*   

  1. 1 Yunnan Key Laboratory of Sugarcane Genetic Improvement / Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661600, China; 2 Biotechnology & Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China
  • Received:2010-03-16 Revised:2010-06-27 Published:2010-11-12 Published online:2010-08-30
  • Contact: FAN Hong-Li,E-mail:fyhysri@vip.sohu.com

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被引次数

14

摘要： 以狼尾草属(Pennisetum Rich.)的象草(P. purpureum) 为外群体，依据rDNA-ITS序列探讨了甘蔗亚族(Saccharinae)内与甘蔗植物分类关系较近的8属37种120份材料的系统进化关系，结果表明，ITS1序列长度为204~208 bp，变异位点91个，简约信息位点70个，GC含量为60.40%~69.10%；ITS2序列长度为215~220 bp，变异位点93个，简约信息位点68个，GC含量为66.10%~73.40%；5.8sDNA序列长度为164 bp, 变异位点18个，简约信息位点9个，GC含量为54.1%~58.0%；根据变异位点，简约信息位点占总位点的比例可以看出，ITS序列比5.8sDNA序列变异程度高，其中ITS1序列又较ITS2序列变异丰富。属种间遗传距离表明芒属(Miscanthus)和荻属(Triarrhena)与甘蔗属(Saccharum)的亲缘关系最近，其次为蔗茅属(Erianthus)和河八王属(Narenga)；而莠竹属(Microstegium)、大油芒属(Spodiopogon)、白茅属(Imperata)与甘蔗属亲缘关系较远。根据甘蔗近缘属种的NJ和MP系统发育关系，支持将斑茅(E. arundinaceus)归入蔗茅属，荻属归入芒属的观点；河八王属的河八王(N. porphyrocoma)与滇蔗茅(E. rockii)亲缘关系较近，而与同属的金猫尾(N. fallax)亲缘关系较远；蔗茅属和芒属属种系统进化关系较其他属种复杂；有4份材料被发现鉴定有误，不应用于后续研究。

关键词: 甘蔗, ITS, 系统进化, 甘蔗亚族

Abstract: Sugarcane related genera and species are important germplasm resources for sugarcane breeding and germplasm innovation. To effectively collect these wild resources and utilize them, ITS (Internal Transcribed Spacer) sequence of 120 accessions which belong to eight genera and thirty seven species were used to analyze their genetic relationships and construct phylogentic relationships with P. purpureum as an outgroup. The results showed the sequence lengths of ITS1, ITS2 and 5.8sDNA of all accessions were 204–208 bp, 215–220 bp, and 164 bp respectively, and their variable sites were 91, 93, and 18, the informative sites were 70, 68, and 9, the GC content was 60.4%–69.1%, 66.1%–73.4% and 54.1%–58.0%. According to the ratio of the variable sites and informative sites to all sites, ITS sequence was richer in variances than 5.8sDNA sequence, and the variances of ITS1 sequence were richer than those of ITS2’s. The genetic distance analysis between sugarcane related genera and species indicated that Miscanthus and Triarrhena were the closest to Saccharum, and the closer ones were Erianthus and Narenga, so the species from these genera should be more considered on collecting sugarcane wild resources in the future; Microstegium, Spodiopogon and Imperata represented farther relationships with Saccharum. The sugarcane related genera and species were separated into ten groups according to the phylogenetic tree of neighbor-joining and maximum parsimony. According to their phylogenetic relationships, E. arundinaceus should be separated from Saccharum and clastified as Erianthus species; Triarrhena shoud be included in Miscanthus; the two species of Narenga did not remain in the same group, but N. porphyrocoma and E. rockii were clustered into the same group, and N. fallax did not belong to any groups, so further researches are needed in identifying the two species. These species from Erianthus and Miscanthus were put in different groups, indicating the very complex genetic relationships within Erianthus and Miscanthus, their species possess big genetic differences. Four samples were found error in classifying species, so the application of their ITS sequences should be avoided. 

Key words: Sugarcane, ITS, Phylogeny, Saccharinae

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