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作物学报 ›› 2011, Vol. 37 ›› Issue (03): 389-396.doi: 10.3724/SP.J.1006.2011.00389

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

棉花叶片早衰的诊断及遗传效应分析

郝俊杰1,刘焕民1,马奇祥1,崔小伟1,于霁雯2,贾新合3,高俊山4   

  1. 1 河南省农业科学院植物保护研究所 / 河南省农作物病虫害防治重点实验室, 河南郑州 450002；2 中国农业科学院棉花研究所, 河南安阳455000；3 郑州市农林科学研究所, 河南郑州 450005；4 开封市农林科学研究院, 河南开封 475004
  • 收稿日期:2010-06-11 修回日期:2010-12-06 出版日期:2011-03-12 网络出版日期:2011-01-17
  • 基金资助:

    本研究由 转基因生物新品种培育重大专项(2008ZX08005-002)和国家棉花产业技术体系和国家科技支撑计划(2006BAD01A05-11)项目资助。

Genetic Effects and Diagnosis of Premature Senescence of Leaf in Upland Cotton

HAO Jun-Jie1,LIU Huan-Min1,MA Qi-Xiang1,CUI Xiao-Wei1,YU Ji-Wen2,JIA Xin-He3,GAO Jun-Shan4   

  1. 1 Plant Protection Institute, Henan Academy of Agricultural Sciences / Henan Key Laboratory for Control of Crop Diseases and Insect Pests, Zhengzhou 450002, China; 2 China Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, China; 3 Zhengzhou Institute of Agriculture and Forestry Sciences, Zhengzhou 450002, China; 4 Kaifeng Academy of Agriculture and Forestry Sciences, Kaifeng 475004, China
  • Received:2010-06-11 Revised:2010-12-06 Published:2011-03-12 Published online:2011-01-17

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1404

被引次数

9

摘要： 比较了利用活体叶片快速、无损害诊断棉花叶片早衰的SPAD差值法和绿色叶面积分级诊断方法，并分析了棉花叶片早衰的数量遗传行为。选用不同叶形和早衰类型的9个棉花品种(系)，比较开花当天倒4叶及以后每5 d一次的SPAD值表明, 早衰棉花品种在开花35 d后SPAD值明显降低，因此，将开花后35 d和开花当天倒4叶的SPAD的差值作为诊断棉花叶片早衰的指标。构建持绿亲本33B和早衰亲本CJ463的6个世代(P1、F1、P2、F2、BC1和BC2)，用联合尺度检验法对其叶片早衰的数据进行世代均值分析，结果表明棉花叶片早衰主要受加性遗传效应控制，至少是一对加性效应的主基因控制陆地棉叶片早衰遗传，且遗传力较高，说明对持绿材料的早代选择是有效的。总之，用SPAD差值和绿色叶面积分级两种诊断叶片早衰的方法来分析叶片早衰遗传及其与叶面积关系的结果基本一致。

关键词: 棉花, SPAD值, 叶片早衰, 遗传效应

Abstract: Premature senescence of cotton has been occurring on an increasing scale in China, directly influencing both yield and fiber quality. The most effective way avoiding premature senescence is to obtain stay-green variety in breeding programs. Moreover, plant breeders demand a simple and efficient diagnosis method, and farther understand the genetic basis of premature senescence in cotton. The objective of this paper was to discuss the quick measurement harmless to plant, and to analyze the genetic effects for leaf premature senescence in upland cotton. The SPAD readings of the fourth leaf from the top were measured at the flowering day and also each five days after flowering in nine cotton varieties (lines). The SPAD readings at 35 days after flowering (DAF) were decreasing in premature  senescence cotton varieties (lines). Therefore, the SPAD difference between 35 DAF and the flowering day was calculated as an indicator of the reduction in chlorophyll content; the greater the rate of reduction, the earlier the senescence, and vice versa. The degree of leaf premature senescence was expressed by the reduction of SPAD or the scale of the green-area in generations (P1, F1, P2, F2, BC1, and BC2) of the 33B×CJ463 cross. Generation mean analyses were conducted to explain the inheritance of leaf premature senescence. The results showed the relative importance of additive effects controlling leaf premature senescence. The estimated minimum number of genes controlling leaf premature senescence was an additive major allele at least with relatively high heritability, suggesting the early selection for late-senescence varieties (lines) was effective in the offspring. The relationships between premature senescence and leaf area per plant were different in the different segregating generations. In a few words, the results of genetic analysis and the relationships between premature senescence and leaf area measured by the reduction of SPAD and the scale of the green-area were relatively consistent.

Key words: Cotton, SPAD reading, Leaf premature senescence, Genetic effects

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