硝普钠缓解花生铝毒害作用和影响AhSAG和AhBI-1基因表达

doi:10.3724/SP.J.1006.2011.00459

摘要/Abstract

摘要： 以花生为材料，研究了铝胁迫诱导花生发生细胞程序性死亡的条件下，加入外源NO供体SNP对不同耐性花生品种根尖生长及相关细胞程序性死亡基因的影响。根长生长、根尖苏木精染色和铝含量测定结果表明，适当浓度的SNP能够缓解一定浓度铝对花生根生长的毒害作用，1.0 mmol L^–1的SNP缓解作用最明显，此浓度下，99-1507和中花2号与对照相比，根长增加45%和52%，根尖铝含量降低10%和23%，苏木精染色变浅。加入1.0 mmol L^–1的SNP处理后，与对应铝胁迫相比，花生衰老基因AhSAG的相对表达量在耐铝品种99-1507中先升高后降低，在0.1 mmol L^–1和0.4 mmol L^–1Al³⁺处理时，显著下降；在铝敏感品种中花2号中呈缓慢升高的趋势，在0.1 mmol L^–1和0.4 mmol L^–1 Al³⁺处理时，显著上升；花生细胞程序性死亡相关基因AhBI-1的相对表达量在99-1507中呈先升高后降低的趋势，在中花2号中则先降低后升高，当99-1507在0和0.02 mmol L^–1 Al³⁺处理时，中花2号在0、0.02和0.1 mmol L^–1 Al³⁺处理时，SNP处理后的AhBI-1表达量均低于对应的单独铝处理。由此说明，NO对花生铝毒害有一定的缓解作用，其作用机理可能与影响细胞程序性死亡基因表达而调控细胞程序性死亡有关。

关键词: 一氧化氮, 花生, 铝胁迫, 缓解效应, 基因表达

Abstract: Recent researches have found that NO (nitric oxide) plays an important role in mediating some biotic and abiotic stress-induced oxidative stresses in plant kingdom. Meanwhile, Al toxicity is a major constraint for crop production in acidic soils worldwide, especially in southern China. In order to enucleate the control effects of NO (nitric oxide) on aluminum (Al) stress, we researched the effects of exogenous NO donor (sodium nitroprusside, SNP) on rootelongation and relative gene expression when Al-induced PCD in root tips happened with peanut (Arachis hypoganea L.) as material. The results of root elongation, hematoxylin dying and root tips Al^{3+ content detection showed that suitable exogenous NO could alleviate Al toxicity on root tips of peanut, especially at 1.0 mmol L^–1SNP. Compared with the control, SNP at 1.0 mmol L^–1 increased root growth 45% and 52%, decreased Al^{3+ content of root tips 10% and 23% in 99-1507 and Zhonghua 2, respectively, in the meanwhile, the color of root tips with hematoxylin dying became light. Compared to Al stress only, relative expression of AhSAG gene in 99-1507(Al-resistant) increased firstly, than decreased after adding SNP at 1.0 mmol L^–1, it decreased significantly in 0.1 and 0.4 mmol L^–1 Al treatments, while that inZhonghua No.2 increased slowly, it increased significantlyin 0.1 and 0.4 mmol L^–1 Al³⁺ treatments. Compared with Al stress only, relative expression of PCD-related AhBI-1 gene in 99-1507 increased firstly, than decreased after adding SNP at 1.0 mmol L^–1, but that in Zhonghua 2 had a reverse change. The relative expression of AhBI-1 gene was lower in Al+SNP treatmentsthan in Al treatments only at 0 and 0.02 mmol L^–1 in 99-1507, and at 0, 0.02, and 0.1 mmol L^–1 in Zhonghua 2. The results showed that NO can alleviate Al toxicity in peanut, and it maybe relate to theregulationof the expression of programmed cell death related genes, while controls the programmed cell death.}}

Key words: Nitric Oxide, Peanut (Arachis hypoganea L.), Aluminum stress, Ameliorating effect, Gene expression

詹洁, 王天菊, 何虎翼, 李创珍, 何龙飞. 硝普钠缓解花生铝毒害作用和影响AhSAG和AhBI-1基因表达[J]. 作物学报, 2011, 37(03): 459-468.

ZHAN Ji, WANG Tian-Ju, HE Hu-Yi, LI Chuang-Zhen, HE Long-Fei. Effects of SNP on AhSAG and AhBI-1 Genes Expression and Amelioration of Aluminum Stress to Peanut (Arachis hypoganea L.)[J]. Acta Agron Sin, 2011, 37(03): 459-468.

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