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作物学报 ›› 2012, Vol. 38 ›› Issue (04): 632-638.doi: 10.3724/SP.J.1006.2012.00632

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

OsGA20ox2不同长度RNAi片段对水稻株高等农艺性状的遗传效应

王坚1,2，赵开军2,*，乔枫2,3，杨生龙1   

  1. 1 宁夏农林科学院农作物研究所, 宁夏银川750105；2 中国农业科学院作物科学研究所 / 农业部作物遗传育种重点实验室 / 农作物基因资源与基因改良国家重大科学工程, 北京100081；3青海师范大学生命与地理科学学院，青海西宁810008
  • 收稿日期:2011-08-01 修回日期:2011-12-19 出版日期:2012-04-12 网络出版日期:2012-02-13
  • 通讯作者: 赵开军, E-mail: zhaokj@mail.caas.net.cn?
  • 基金资助:

    本研究由宁夏自然科学基金项目(NZ0984)资助。

Genetic Effects of Different RNA Interference Fragments from OsGA20ox2 on Plant Height and Other Agronomic Traits in Rice

WANG Jian1,2, ZHAO Kai-Jun2,*, QIAO Feng2,3,YANG Sheng-Long1   

  1. 1 Crop Institute of Ningxia Academy of Agriculture and Forestry Sciences, Ningxia 750105, China; 2 Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture / National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Life and Geography Sciences, Qinghai Normal University, Xining 810008, China?
  • Received:2011-08-01 Revised:2011-12-19 Published:2012-04-12 Published online:2012-02-13
  • Contact: 赵开军, E-mail: zhaokj@mail.caas.net.cn?

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摘要： 利用OsGA20ox2基因序列构建不同长度的RNAi片段并导入水稻，获得不同高度的矮化植株。将这些矮化植株与野生型植株回交获得B1F2群体，卡方检测表明B1F2群体矮秆植株数和高秆植株数符合3∶1比例，表现为矮秆显性的遗传规律。对矮化植株的F5和B1F2群体株高、各茎节间长度和一些主要农艺性状方差分析显示, OsGA20ox2基因的RNAi能显著缩短株高和各节间长度(P<0.05)，RNAi干扰片段越长，使植株株高和节间长度缩短程度越大，可使株高降低24~42 cm，矮化22%~39%。在同一长度的RNAi干扰片段下，倒一节节间长度平均缩短与倒二节节间长度平均缩短非常相近，倒三节和倒四节节间长度平均缩短非常相近，总的缩短程度是倒四节>倒三节>倒二节>倒一节。这种近基部节间长度缩短幅度和比例较大的特点，利于提高水稻的抗倒伏能力，同时上部节间缩短幅度和比例较小，有效地保持合理株高，不使生物产量明显降低，有利于水稻的稳产和高产。OsGA20ox2基因的RNAi不影响如千粒重、结实率、穗长等其他主要农艺性状或影响很小。

关键词: RNAi, 株高, 节间长度, 遗传效应

Abstract: In our previous study, different rice dwarf lines were obtained through rice genetic transformation using RNAi vectors harboring different fragments of OsGA20ox2 gene. In this study, B1F2 populations were produced by backcrossing between those RNAi dwarf lines and wild-type plants. The progenies of B1F2 populations segregated in a ratio of 3 dwarf plants to 1 normal height plant, indicating that dwarf trait was dominantly inherited. At the same time, plant height, the internode length and some major agronomic traits of dwarf plants of F5 and B1F2 populations were analyzed. It was resulted that RNAiof OsGA20ox2 gene significantly reduced the rice plant height and the internode length. The longer the interference fragment was, the shorter the plant height and internode length were. In general, the rice plant height was reduced by 24–42 cm, dwarfing ratio was 22–39%. For the same RNAi fragment, the average shorten length of the first internode (counting from the top to bottom) was very similar to that of the second, and the third was very similar to the fourth. The dwarfing ratio of each internode showed a trend of the fourth > third > second > first section. Bottom internodes obtained a greater dwarfing ratio, which is good for improving lodging resistance of rice, and the upper internodes showed a lower dwarfing ratio that made the plant maintain a reasonable height, which helps the rice plants to remain a stable and high yield. Other major agronomic traits such as grain weight, seed setting rate, panicle length were not significantly affected by the RNAiof OsGA20ox2 gene.

Key words: RNAi, Plant height, Length of internode, Genetic effect

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