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作物学报 ›› 2012, Vol. 38 ›› Issue (07): 1339-1345.doi: 10.3724/SP.J.1006.2012.01339

• 研究简报 • 上一篇    

12个小麦品种(系)白粉病抗性的遗传分析

宋凤景1，肖明纲1，黄江1,2,3，王晓鸣1，朱振东1，武小菲1，李洪杰1,*   

  1. 1 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程, 北京100081; 2 中国科学院遗传与发育生物学研究所农业资源研究中心农业水资源重点实验室，河北石家庄 050021; 3 中国科学院研究生院，北京 100049
  • 收稿日期:2011-12-30 修回日期:2012-04-16 出版日期:2012-07-12 网络出版日期:2012-05-11
  • 通讯作者: 李洪杰, E-mail: hongjie@caas.net.cn
  • 基金资助:

    本研究由中央级公益性科研院所基本科研业务费专项(20105), 国家现代农业产业技术体系建设项目(CARS-3-1)和国家转基因生物新品种培育重大专项(2009ZX08002-006B)的资助。

Inheritance of Resistance to Powdery Mildew in 12 Wheat Cultivars

SONG Feng-Jing1,XIAO Ming-Gang1,HUANG Jiang1,2,3,WANG Xiao-Ming1,ZHU Zhen-Dong1,WU Xiao-Fei1,LI Hong-Jie1,*   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China; 2 Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050021, China; 3 Graduate School of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-12-30 Revised:2012-04-16 Published:2012-07-12 Published online:2012-05-11
  • Contact: 李洪杰, E-mail: hongjie@caas.net.cn

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1853

被引次数

30

摘要： 利用17个不同来源和毒力的白粉菌菌株对12个小麦品种(系)进行苗期抗性鉴定和抗病性遗传分析，同时利用Pm2和Pm8基因的特异分子标记检测了相应基因。供试的12个品种至少能够抗11个白粉菌菌株。用E09、E20和Bg2菌株接种F2群体，抗感植株分离比例和适合性测验证明这12个品种对不同白粉菌菌株的抗性均受1对显性基因控制。抗谱分析和基因紧密连锁分子标记(Xcfd81)分析表明良星66很可能含有Pm2或其等位基因。ω-黑麦碱基因(1RS染色体)和Glu-B1基因(1BS染色体)特异分子标记分析结果证明，山农20和郑麦9962含有T1BL·1RS易位染色体，即可能携带Pm8基因。由于Pm8基因对大多数菌株表现感病，所以这2个品种除Pm8外，还具有其他抗病基因。偃展4110与天民668对参试菌株的反应型表现一致，其他材料对不同菌株的反应型表现不同。

关键词: 小麦, 白粉病, 抗病基因, 遗传分析

Abstract: Wheat powdery mildew, caused by the biotrophic parasitic fungus Blumeria graminis (DC.) f. sp. tritici E.O. Speer. is one of the most epidemic diseases in many wheat (Triticum aestivum L.) producing regions in China and other parts of the world. The information on inheritance of resistance to powdery mildew in commercial wheat cultivars is important for controlling the disease and developing new resistant cultivars. In the present study, 17 isolates of B. graminis were used to postulate and analyze powdery mildew resistance genes in 12 wheat cultivars at seedling stage. Molecular detection was performed to disclose the presence of Pm2 and Pm8 for powdery mildew resistance. All of the 12 wheat cultivars were resistant to at least 11 tested isolates. Isolates E09, E20, and Bg2 were used to test F2 populations for analyzing inheritance of powdery mildew resistance in these wheat cultivars. The results demonstrated that single dominant gene(s) was responsible for powdery mildew resistance in these cultivars. The reactions to different isolates of B. graminis and analysis of amplification with Pm2-linked marker Xcfd81showed that Pm2 or its allele was most likely present in Liangxing 66. Using ω-secalin gene- and Glu-B1 gene-specific markers on chromosome arms 1RS and 1BS, respectively, the presence of T1BL·1RS translocation chromosome carrying Pm8 was detected in Shannong 20 and Zhengmai 9962. Since Pm8 was not effective against most isolates tested in this study, other unknown genes for powdery mildew resistance could be present in Shannong 20 and Zhengmai 9962 in addition to Pm8. The reaction patterns of Yanzhan 4110 and Tianmin 668 were identical. The remaining cultivars developed different patterns of reaction to the B. graminis isolates tested.

Key words: Wheat, Powdery mildew, Resistance gene, Inheritance

[1]Zhuang Q-S(庄巧生). Wheat Improvement and Pedigree Analysis in China (中国小麦品种改良及系谱分析). Beijing: China Agriculture Press, 2003 (in Chinese)

[2]Zhan H-X(詹海仙), Chang Z-J(畅志坚), Yang Z-J(杨足君), Zhang H-J(张晖军), Li X(李欣). Sources and evaluation of powdery mildew resistance genes in wheat. Chin Agric Sci Bull (中国农学通报), 2010, 26(10): 42–46 (in Chinese with English abstract)

[3]Wu X-H(吴先华), Luo P-G(罗培高), Yan B-J(晏本菊), Ren Z-L(任正隆). Researches on Powdery mildew resistance genes and its breeding in wheat. Chin Agric Sci Bull (中国农学通报), 2006, 22(5): 346–351 (in Chinese with English abstract)

[4]Zhang H-Q(张海泉). Research advances in molecular breeding of powdery mildew resistance of wheat. Chin J Eco-Agric (中国生态农业学报), 2008, 16(4): 1060–1066 (in Chinese with English abstract)

[5]Li G P, Chen P D, Zhang S Z, Wang X E, He Z H, Zhang Y, Zhao H, Huang H Y, Zhou X C. Effect of the 6VS.6AL translocation on agronomic traits and dough properties of wheat. Euphytica, 2007, 155: 305–313

[6]Wang Z L, Li L H, He Z H, Duan X Y, Zhou Y L, Chen X M, Lillemo M, Singh R P, Wang H., Xia X C. Seedling and adult plant resistance to powdery mildew in Chinese bread wheat cultivars and lines. Plant Dis, 2005, 89: 457–463

[7]Li H-J(李洪杰), Wang X-M(王晓鸣), Song F-J(宋凤景), Wu C-P(伍翠平), Wu X-F(武小菲), Zhang N(张宁), Zhou Y(周阳), Zhang X-Y(张学勇). Response to powdery mildew and detection of resistance genes in wheat cultivars from China. Acta Agron Sin (作物学报), 2011, 37(6): 943–954 (in Chinese with English abstract)

[8]Wu Q-A(吴全安). Methods Used in the Evaluation of Pest Resistant Potentialities in Food Crop Germplasm Resources (粮食作物种质资源抗病虫鉴定方法). Beijing: China Agriculture Press, 1991 (in Chinese)

[9]Qiu Y C, Sun X L, Zhou R H, Kong X Y, Zhang S S, Jia J Z. Identification of microsatellite markers linked to powdery mildew resistance gene Pm2 in wheat. Cereal Res Commun, 2006, 34: 1267–1273

[10]Chai J F, Zhou R H, Jia J Z, Liu X. Development and application of a new codominant PCR marker for detecting 1BL.1RS wheat-rye chromosome translocations. Plant Breed, 2006, 125: 302–304

[11]Cao X-R(曹学仁), Zhou Y-L(周益林), Duan X-Y(段霞瑜), Song Y-L(宋玉立), He W-L(何文兰), Ding K-J(丁克坚), Wang B-T(王保通), Xia X-C(夏先春). Postulation of wheat powdery mildew resistance genes in commercial wheat cultivars and advanced lines from Gansu Province. J Triticeae Crops (麦类作物学报), 2011, 30(5): 948–953 (in Chinese with English abstract)

[12]Li H J, Conner R L, Liu Z Y, Li Y W, Chen Y, Zhou Y L, Duan X Y, Shen T M, Chen Q, Graf R J, Jia X. Characterization of wheat-triticale lines resistant to powdery mildew, stem rust, stripe rust, wheat curl mite, and limitation on spread of WSMV. Plant Dis, 2007, 91: 368–374

[13]Hu T-Z(胡铁柱), Li H-J(李洪杰), Liu Z-J(刘子记), Xie C-J(谢超杰), Zhou Y-L(周益林), Duan X-Y(段霞瑜), Jia X (贾旭), You M-S(尤明山), Yang Z-M(杨作明), Sun Q-X(孙其信), Liu Z-Y(刘志勇). Identification and molecular mapping of the powdery mildew resistance gene in wheat cultivar Yumai 66. Acta Agron Sin (作物学报), 2008, 34(4): 545–550 (in Chinese with English abstract)

[14]Niu J S Wang B Q, Wang Y H, Cao A Z, Qi Z J, Shen T M. Chromosome location and microsatellite markers linked to a powdery mildew resistance gene in wheat line ‘Lankao 90(6)’. Plant Breed, 2008, 127: 346–349
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