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作物学报 ›› 2012, Vol. 38 ›› Issue (07): 1334-1338.doi: 10.3724/SP.J.1006.2012.01334

• 研究简报 • 上一篇    下一篇

利用比较基因组学开发山羊草属InDel分子标记

吴磊，王丹，苏文悦，郭长虹*，束永俊*   

  1. 哈尔滨师范大学生命科学与技术学院 / 黑龙江省分子细胞遗传与遗传育种重点实验室, 黑龙江哈尔滨 150025
  • 收稿日期:2012-02-14 修回日期:2012-04-20 出版日期:2012-07-12 网络出版日期:2012-05-11
  • 通讯作者: 束永俊, Email: syjun2003@126.com; 郭长虹, Email: kaku3008@yahoo.com.cn
  • 基金资助:

    本研究由国家重点基础研究计划(973计划)前期项目(2011CB111505), 黑龙江省青年科学基金项目(QC2011C108), 国际科技合作项目(2009DFA32470),黑龙江省教育厅科学技术研究项目(12521149)和哈尔滨师范大学科技创新团队(KJTD201102)资助。

Developing InDel Markers from Aegilops Genus Based on Comparative Genomics

WU Lei, WANG Dan,SU Wen-Yue,GUO Chang-Hong*,SHU Yong-Jun*   

  1. Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province/College of Life Science and Technology, Harbin Normal University, Harbin 150025, China
  • Received:2012-02-14 Revised:2012-04-20 Published:2012-07-12 Published online:2012-05-11
  • Contact: 束永俊, Email: syjun2003@126.com; 郭长虹, Email: kaku3008@yahoo.com.cn

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1852

被引次数

13

摘要： 为开发和利用小麦野生近缘种的有益基因, 采用比较基因组学方法, 通过拟斯卑尔脱山羊草EST(expressed sequence tag)与小麦UniGene序列的比对分析, 发现山羊草插入/缺失(InDel)位点137个, 在这些位点两端序列设计引物24对, 通过在15个小麦野生近缘属种基因组DNA的扩增分析, 发现11对引物具多态性, 可以作为InDel标记。这些包含突变位点的基因涉及亚细胞定位、蛋白质结合与催化以及代谢等过程。

关键词: 比较基因组学, 插入/缺失突变, 小麦, 拟斯卑尔脱山羊草, 功能分子标记

Abstract: The expressed sequence tags (ESTs) of Aegilops speltoides were aligned with UniGene sequences of wheat to develop molecular markers for favorable genes that can be used in wheat breeding. A total of 137 insertion/deletion (InDel) sites were identified in Ae. speltoides, and 24 pairs of primers flanking these InDel sites were designed. Of the 24 pairs of primer, 11 had polymorphic amplification in 15 species of wheat relatives, suggesting that they can be used as InDel markers. These InDel markers were functional markers involved in subcellular localization, protein binding or catalyzing, metabolic process and cell rescue, defense, and disease resistance.

Key words: Comparative genomics, Insertion/deletion mutation (InDel), Wheat, Aegilops speltoides, Functional molecular marker

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