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作物学报 ›› 2012, Vol. 38 ›› Issue (08): 1397-1406.doi: 10.3724/SP.J.1006.2012.01397

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻叶色白化转绿及多分蘖矮秆基因hw-1(t)的图位克隆

郭涛**，黄永相**，黄宣，刘永柱，张建国，陈志强*，王慧*   

  1. 华南农业大学 / 国家植物航天育种工程技术研究中心, 广东广州510642
  • 收稿日期:2012-01-06 修回日期:2012-04-16 出版日期:2012-08-12 网络出版日期:2012-06-04
  • 通讯作者: 王慧, E-mail: wanghui@scau.edu.cn; 陈志强, E-mail: chenlin@scau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(30771313)和国家科技支撑计划项目(2008BAD97B02)资助。

Map-Based Cloning of a Green-Revertible Albino and High-Tillering Dwarf Gene hw-1(t) in Rice

GUO Tao**,HUANG Yong-Xiang**,HUANG Xuan,LIU Yong-Zhu,WANG Hui,ZHANG Jian-Guo,CHEN Zhi-Qiang*,WANG Hui*   

  1. South China Agricultural University, National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China
  • Received:2012-01-06 Revised:2012-04-16 Published:2012-08-12 Published online:2012-06-04
  • Contact: 王慧, E-mail: wanghui@scau.edu.cn; 陈志强, E-mail: chenlin@scau.edu.cn

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2115

被引次数

11

摘要： 通过增加作图群体的样本量, 将控制水稻白化转绿和多分蘖矮秆的基因hw-1(t)定位在第4染色体长臂2个InDel标记HW36和HW7之间24.9 kb的物理距离内, 该区域含有5个阅读框架。测序及酶切分析表明, 突变体hfa-1仅在LOC_Os04g57320产生一个碱基(G→A)的突变, 导致翻译提取终止, 推断LOC_Os04g57320为hw-1(t)。进一步分析发现hw-1(t)在水稻基因组中为单拷贝, 与拟南芥im和番茄PTOX基因同源性较高, 但在转录子和蛋白质结构上存在一定差异；基因表达分析显示HW-1(t)属组成型表达基因, 表达不受光照影响。

关键词: 水稻, 白化转绿, 多分蘖矮秆, hw-1(t), 克隆

Abstract: In this study, the green-revertible albino and high-tillering dwarf gene hw-1(t) was fine mapped with a 24.9 kb physical distance between two InDel markers, HW36 and HW7 on chromosome 5, where five open reading frames were predicted. Sequencing and restriction enzyme digestion analysis revealed hfa-1 mutant carried a single nucleotide substitution (G→A) in the putative gene LOC_Os4g57320, which resulted in a premature stop codon. Therefore, we deduced that LOC_Os4g57320 is hw-1(t). BLAST analysis indicated that hw-1(t) exits as a single copy in rice genome. Sequence alignment revealed that hw-1(t) was highly homologous to AtIM and SiPTOX, except some variation on transcript and protein structure. Gene expression analysis indicated HW-1(t) was constitutive gene, which did not be affected by light.

Key words: Rice (Oryza sativa L.), Green-revertible albino, High-tillering dwarf, hw-1(t), Cloning

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