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作物学报 ›› 2012, Vol. 38 ›› Issue (11): 1969-1976.doi: 10.3724/SP.J.1006.2012.01969

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

普通小麦–簇毛麦种质对菲利普孢囊线虫的抗性分析

张佳佳1,袁虹霞1,张瑞奇2,邢小萍1,代君丽1,牛吉山1,李洪连1,*,陈佩度2,*   

  1. 1河南农业大学植物保护学院, 河南郑州 450002;2南京农业大学细胞遗传研究所, 江苏南京210095
  • 收稿日期:2012-04-05 修回日期:2012-07-05 出版日期:2012-11-12 网络出版日期:2012-09-10
  • 通讯作者: 李洪连, E-mail: honglianli@sina.com; 陈佩度, E-mail: pdchen@njau.edu.cn
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(200903040-4)和国家高技术研究发展计划(863计划)项目(2012AA101105)资助。

Analysis of Resistance to Heterodera filipjevi in Triticum aestivum–Dasypyrum villosum Germplasm

ZHANG Jia-Jia1,YUAN Hong-Xia1,ZHANG Rui-Qi2,XING Xiao-Ping1,DAI Jun-Li1,NIU Ji-Shan1,LI Hong-Lian1,*,CHEN Pei-Du2,*   

  1. 1 College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China; 2 Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-04-05 Revised:2012-07-05 Published:2012-11-12 Published online:2012-09-10
  • Contact: 李洪连, E-mail: honglianli@sina.com; 陈佩度, E-mail: pdchen@njau.edu.cn

摘要:

菲利普孢囊线虫(Heterodera filipjevi)是在我国新发现的一种小麦病原线虫, 已严重威胁我国小麦的安全生产。小麦近缘种属具有改良小麦所需的许多目标性状, 是丰富小麦遗传变异、选育抗病品种的重要基因资源。采用室内接种鉴定法, 从20份小麦近缘属种材料中发现簇毛麦(Dasypyrum villosum)高抗H. filipjevi。分别对3套普通小麦–簇毛麦染色体附加系和6VS易位系进行接虫抗性鉴定, 结果6V染色体附加系高抗H. filipjevi;含6VS的易位系则表现感病。据此推测, 簇毛麦6V染色体上可能含有抗H. filipjevi基因, 且可能位于6VL上。

关键词: 小麦近缘属种, 簇毛麦, 菲利普孢囊线虫, 原位杂交, 基因定位

Abstract:

Heterodera filipjevi is a new pathogenic species of cereal cyst nematode (CCN) in China, which has a potential threat to wheat production security. Wheat relatives are important resources of multiple resistance genes to many biotic and abiotic stresses, including CNN resistance genes. Using relative resistance index (RRI) as the evaluation indicator, we screened resistant resources against H. filipjevi from 20 wheat relatives after artificial inoculation, and found Dasypyrum villosum highly and stably resistant to H. filipjevi Xuchang population. Three sets of common wheat (Triticum aestivum L. cv. Chinese Spring) chromosome addition lines with individual D. villosum chromosome 1V to 7V, as well as 6VS translocation lines from different origins, were evaluated for CCN resistance. The Triticum aestivum–D. villosum 6V chromosome addition line showed high resistance to CCN with RRI higher than 0.90 in all sets of addition lines; however, the translocation lines 6VS were susceptible to CNN with RRI ranging from 0.43 to 0.51. Therefore, the CCN resistance gene is most probably located on chromosome 6VL.

Key words: Wheat relatives, Dasypyrum villosum, Heterodera filipjevi, GISH, Gene location

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