作物学报 ›› 2013, Vol. 39 ›› Issue (01): 43-49.doi: 10.3724/SP.J.1006.2013.00043
姚坚强1,2,鲍坚东2,3,朱金庆1,桂毅杰2,沈秋芳2,胡伟民2,樊龙江2,*
YAO Jian-Qiang1,2,BAO Jian-Dong2,3,ZHU Jin-Qing1,GUI Yi-Jie2,SHEN Qiu-Fang2,HU Wei-Min2,FAN Long-Jiang2,*
摘要:
Waxy(Wx)基因是众多作物导致糯性突变的关键基因,在玉米作物群体内深入研究Wx基因对中国糯玉米品质育种和种质创新具有重要意义。本研究利用325份(309份来自中国,11份来自泰国和5份来自韩国)糯玉米种质材料做了wx基因突变类型的调查和表观直链淀粉含量(AAC)测定。结果表明, 中国糯玉米的wx基因的遗传多样性很低,主要存在wx-D7和wx-D10两种突变类型,占96.9%;中国北方及韩国的糯玉米wx基因突变类型都是wx-D7类型,南方及泰国糯玉米wx基因突变类型都是wx-D10类型;糯玉米地方品种的wx基因型以wx-D10为主,占78.9%,而商业杂交种和自交系的wx基因型以wx-D7为主,分别占88.7%和86.6%。分析表明,中国糯玉米商业杂交种和自交系的AAC均值较低(小于2.2%)且无显著差异,但地方品种的AAC均值较高;wx-D7基因型糯玉米的AAC均值低且变化幅度较小,而wx-D10基因型糯玉米的AAC均值最高且变化幅度较大。本文还讨论了中国糯玉米主要wx基因型的地理分布、起源以及其他的wx基因突变类型。
| [1]Fedoroff N, Wessler S, Shure M. Isolation of the transposable maize controlling elements Ac and Ds. Cell, 1983, 35: 235–242[2]Hirano H Y, Sano Y. Molecular characterization of the waxy locus of rice (Oryza sativa). Plant Cell Physiol, 1991, 32: 989–997[3]Wang Z Y, Zheng F Q, Shen G Z, Gao J P, Snustad D P, Li M G, Zhang J L, Hong M M. The amylose content in rice endosperm is related to the posttranscriptional regulation of the waxy gene. Plant J, 1995, 7: 613–622[4]Hirano H Y, Eiguchi M, Sano Y. A single base change altered the regulation of the waxy gene at the posttranscriptional level during the domestication of rice. Mol Biol Evol, 1998, 15: 978–987 [5]Isshiki M, Morino K, Nakajima M, Okagaki R J, Wessler S R, Izawa T, Shimamoto K. A naturally occurring functional allele of the rice waxy locus has a GT to TT mutation at the 5’ splice site of the first intron. Plant J, 1998, 15: 133–138[6]Olsen K M, Purugganan M D. Molecular evidence on the origin and evolution of glutinous rice. Genetics, 2002, 162: 941–950[7]McIntyre C L, Drenth J, Gonzalez N, Henzell R G, Jordan D R. Molecular characterization of the waxy locus in sorghum. Genome, 2008, 51: 524–533 [8]Domon E, Fuijita M, Ishikawa N. The insertion/deletion polymorphisms in the waxy gene of barley genetic resources from east Asia. Theor Appl Genet, 2002, 104: 132–138[9]Patron N J, Smith A M, Fahy B F, Hylton C M, Naldrett M J, Rossnagel B G, Denyer K. The altered pattern of amylose accumulation in the endosperm of low-amylose barley cultivars is attributable to a single mutant allele of granule-bound starch synthase i with a deletion in the 5'-non-coding region. Plant Physiol, 2002, 130: 190–198[10]Hunt H V, Denyer K, Packman L C, Jones M K, Howe C J. Molecular basis of the waxy endosperm starch phenotype in broomcorn millet (Panicum miliaceum L.). Mol Biol Evol, 2010, 27: 1478–1494[11]Nakamura T, Yamamori M, Hirano H, Hidaka S, Nagamine T. Production of waxy (amylose-free) wheats. Mol Gen Genet, 1995, 248: 253-259[12]Wessler S R, Varagona M J. Molecular basis of mutations at the waxy locus of maize: correlation with the fine structure genetic map. Proc Natl Acad Sci USA, 1985, 82: 4177–4181[13]Wessler S, Tarpley A, Purugganan M, Spell M, Okagaki R. Filler DNA is associated with spontaneous deletions in maize. Proc Natl Acad Sci USA, 1990, 87: 8731–8735[14]Marillonnet S, Wessler S R. Retrotransposon insertion into the maize waxy gene results in tissue-specific RNA processing. Plant Cell, 1997, 9: 967–978[15]Liu J, Rong T Z, Li W C. Mutation loci and intragenic selection marker of the granule-bound starch synthase gene in waxy maize. Mol Breed, 2007, 20: 93–102[16]Klosgen R B, Gierl A, Schwarzsommer Z, Saedler H. Molecular analysis of the waxy locus of Zea mays. Mol Biol Evol, 1986, 203: 237–244[17]Fan L J, Quan L Y, Leng X D, Guo X Y, Hu W M, Ruan S L, Ma H S, Zeng M Q. Molecular evidence for post-domestication selection in the waxy gene of Chinese waxy maize. Mol Breed, 2008, 22: 329–338[18]Fan L J, Bao J D, Wang Y, Yao J Q, Gui Y J, Hu W M, Zhu J Q, Zeng M Q, Li Y, Xu Y B. Post-domestication selection in the maize starch pathway. PLoS One, 2009, 4: e7612[19]Tian M-L(田孟良), Huang Y-B(黄玉碧), Tan G-X(谭功燮), Liu Y-J(刘永建), Rong T-Z(荣廷昭). Sequence polymorphism of waxy genes in landraces of waxy maize from Southwest China. Acta Agron Sin (作物学报), 2008, 34(5): 729–736 (in Chinese with English abstract)[20]Okagaki R J, Neuffer M G, Wessler S R. A deletion common to two independently derived waxy mutations of maize. Genetics, 1991, 128: 425–431[21]Huang Y-B(黄玉碧), Rong T-Z(荣廷昭). Genetic diversity and origin of Chinese waxy maize. Crop (作物杂志), 1998, (suppl): 77–80 (in Chinese)[22]Zeng M-Q(曾孟潜), Yang T-X(杨太兴), Wang P(王璞). The relative analyses on maize cultivar Menghai Four-Row Wax. Acta Genet Sin (遗传学报), 1981, 8(1): 91-96 (in Chinese with English abstract)[23]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321-4325[24]Shin J H, Kwon S J, Lee J K, Min H K, Kim N S. Genetic diversity of maize kernel starch-synthesis genes with SNAPs. Genome, 2006, 49: 1287–1296[25]Bao J D, Yao J Q, Zhu J Q, Hu W M, Cai D G, Li Y, Shu QY, Fan L J. Identification of glutinous maize landraces and inbred lines with altered transcription of waxy gene. Mol Breed, 2012 (Published online: DOI 10.1007/s11032-012-9754-3) |
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