作物学报 ›› 2013, Vol. 39 ›› Issue (03): 423-430.doi: 10.3724/SP.J.1006.2013.00423
蔺芳芳,杨旭,武小翠,刘晓梅,葛荣朝,赵宝存*
LIN Fang-Fang,YANG Xu,WU Xiao-Cui,LIU Xiao-Mei,GE Rong-Chao,ZHAO Bao-Cun*
摘要:
TaSC (Triticum asetivum L. salt-tolerance related gene, GenBank 登录号为AY956330)是从小麦耐盐突变体RH8706-49 中克隆的高盐诱导表达的耐盐相关基因。以该基因编码的蛋白质TaSC 为诱饵, 运用分裂泛素酵母双杂交技术从小麦cDNA 表达文库中钓取互作蛋白质, 筛选到一个编码小麦未知功能蛋白质的基因(GenBank 登录号为AK336035), 命名为TaSCIP1 (TaSC interaction protein 1)。双分子荧光互补(BiFC)实验证实TaSCIP1 与TaSC 存在互作。该互作蛋白的分离有利于进一步研究TaSC 基因的耐盐机制。
| [1]Zhu M-Y(朱睦元), Huang P-Z(黄培忠). Barley Breeding and Bioengineering (大麦育种与生物工程). Shanghai: Shanghai Scientific and Technical Publishers, 1999. pp 202–210 (in Chinese) [2]Huang G T, Ma S L, Bai L P, Zhang L, Ma H, Jia P, Liu J, Zhong M, Guo Z F. Signal transduction during cold, salt, and drought stresses in plants. Mol Biol Rep, 2012, 39: 969–987[3]Fu J, Zhang D F, Liu Y H, Ying S, Shi Y S, Song Y C, Li Y, Wang T Y. Isolation and characterization of maize PMP3 genes involved in salt stress tolerance. PLoS One, 2012; 7(2): e31101[4]Zhang H X, Blumwald E. Transgenic salt-tolerant tomato Plants accumulate salt in foliage but not in fruit. Nat. Biotechnol., 2001, 19: 765–768[5]Katiyar-Agarwal S, Zhu J, Kim K, Agarwal M, Fu X, Huang A, Zhu J K. The plasma membrane Na+/H+ antiporter SOS1 interacts with RCD1 and functions in oxidative stress tolerance in Arabidopsis. Proc Natl Acad Sci USA, 2006, 103: 18816–18821[6]Sade N, Gebretsadik M, Seligmann R, Schwartz A, Wallach R, Moshelion M. The role of tobacco Aquaporin 1 in improving water use efficiency, hydraulic conductivity, and yield production under salt stress. Plant Physiol, 2010, 152: 245–254[7]Giri J, Vij S, Dansana P K, Tyagi A K. Rice A20/AN1 zinc-finger containing stress-associated proteins (SAP1/11) and a receptor-like cytoplasmic kinase (OsRLCK253) interact via A20 zinc-finger and confer abiotic stress tolerance in transgenic Arabidopsis plants. New Phytol, 2011, 191: 721–732[8]Johnsson N, Varshavsky A. Split ubiquitin as a sensor of protein interactions in vivo. Proc Natl Acad Sci USA. 1994, 91: 10340–10344[9]Reinders A, Schulze W, Kühn C, Barker L, Schulz A, Ward J M, Frommer W B. Protein-protein interactions between sucrose transporters of different affinities colocalized in the same enucleate sieve element. Plant Cell, 2002, 14: 1567–1577[10]Stagljar I, Korosotensky C, Johnsson N, te Heesen S. A genetic system based on split-ubiquitin for analysis of interacton between membrane proteins in vivo. Proc Natl Acad Sci USA, 1998, 95: 5187–5192[11]Thaminy S, Auerbach D, Arnoldo A, Stagljar I. Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system. Genome Res, 2003, 13: 1744–1753[12]Wang B, Nguyen M, Breckenridge D G, Stojanovic M, Clemons P A, Kuppig S, Shore G C. Uncleaved BAP31 in association with A4 protein at the endoplasmic reticulum is an inhibitor of Fas-initiated release of cytochrome c from mitochondria. J Biol Chem, 2003, 278: 14461–14468[13]Bracha-Drori K, Shichrur K, Katz A, Oliva M, Angelovici R, Yalovsky S, Ohad N. Detection of protein-protein interactions in plants using bimolecular fluorescence complementation. Plant J, 2004, 40: 419–427[14]Ge RC,Chen GP, Zhao BC, Shen YZ, Huang ZJ. Cloning and functional characterization of a wheat serine/threonine kinase gene (TaSTK) related to salt-resistance. Plant Sci, 2007, 173: 55–60[15]Huang X, Zhang Y, Jiao B, Chen G P, Huang S H, Guo F, Shen Y Z, Huang Z J, Zhao B C. Overexpression of the wheat salt tolerance-related gene TaSC enhances salt tolerance in Arabidopsis. J Exp Bot, 2012, 63: 5463–5473[16]Walter M, Chaban C, Schutze K, Batistic O, Weckermann K, Nake C, Blazevic D, Grefen C, Schumacher K, Oecking C, Harter K, Kudla J. Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J, 2004, 40: 428–438[17]Wang B-S(王宝山), Zou Q(邹琦). Advances in the study on plasma membrane-bound translocating proteins and their relations with salt tolerance in plants. Chin Bull Bot (植物学通报), 2000, 17(1): 17–26 (in Chinese)[18]Gouiaa S, Khoudi H, Leidi E O, Pardo J M, Masmoudi K. Expression of wheat Na(+)/H(+) antiporter TNHXS1 and H(+)-pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance. Plant Mol Biol, 2012, 79: 137–155[14]Qiu Q S, Guo Y, Dietrich M A, Schumaker K S, Zhu J K. Regulation of SOS1, a plasma membrane Na+/H+ exchanger in Arabidopsis thaliana, by SOS2 and SOS3. Proc Natl Acad Sci USA, 2002, 99: 8436–8441 [20]Shi H, Ishitani M, Kim C, Zhu J K. The A rabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter. Proc Natl Acad Sci USA, 2000, 97: 6896–6901[21]Li R, Zhang J, Wu G, Wang H, Chen Y, Wei J. HbCIPK2, a novel CBL-interacting protein kinase from halophyte Hordeum brevisubulatum, confers salt and osmotic stress tolerance. Plant Cell Environ, 2012, 35: 1582–1600[22]Yang L, Ji W, Gao P, Li Y, Cai H, Bai X, Chen Q, Zhu Y M. GsAPK, an ABA-activated and calcium-independent SnRK2-type kinase from G. soja, mediates the regulation of plant tolerance to salinity and ABA stress. PLoS One, 2012, 7: e33838 |
| [1] | 翟胜男, 曹新有, 李豪圣, 李吉虎, 李法计, 刘金栋, 夏先春, 吕莹莹, 马瑞峰, 王颖, 耿洪伟, 刘建军. 小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J]. 作物学报, 2026, 52(6): 1593-1603. |
| [2] | 习千辉, 徐梓瑗, 刘梦梦, 王宏艺, 郎凯琳, 井震海, 陈锋, 赵磊. 小麦籽粒铜含量的全基因组关联分析及候选基因预测[J]. 作物学报, 2026, 52(6): 1604-1617. |
| [3] | 毛嘉琦, 黄朋雨, 赵佳佳, 郑兴卫, 武棒棒, 郝宇琼, 屈非, 刘成, 马朋涛, 郑军. 山西小麦品种白粉病抗性评价及抗病基因分子检测[J]. 作物学报, 2026, 52(6): 1669-1681. |
| [4] | 胡川, 赵凯男, 黄修利, 吴金芝, 任开明, 王贺正, 付国占, 黄明, 李友军. 一次灌溉下耕作方式和氮肥用量对旱地小麦产量和品质的影响[J]. 作物学报, 2026, 52(6): 1830-1846. |
| [5] | 陈雪燕, 何华川, 李政嘉, 董新盼, 李藕琪, 刘小云, 李丹萍, 陈志伟, 刘国霞, 吕胜源, 吴印莹, 赵振东, 曹新有, 万何平. 水培盐碱复合胁迫下‘济麦60’苗期根系有机酸分泌动态变化及其转录调控机制[J]. 作物学报, 2026, 52(6): 1859-1875. |
| [6] | 高沛阳, 李瑾璇, 董宇奎, 石玉, 张振, 张永丽. 测墒补灌下小麦分蘖发生和成穗对施氮量的响应[J]. 作物学报, 2026, 52(6): 1847-1858. |
| [7] | 张献丰, 郭利建, 李康春, 孔斌雪, 刘玉芳, 车卓, 杨德龙. 小麦ABHD6基因家族鉴定与粒重功能标记开发[J]. 作物学报, 2026, 52(6): 1711-1727. |
| [8] | 王壮壮, 武紫君, 张永新, 张芯源, 袁丽雪, 陈如雪, 刘世举, 段剑钊, 冯伟, 王同朝, 王永华. 豫东南黏壤潮土区水氮优化协同提高冬小麦产量和氮素利用效率[J]. 作物学报, 2026, 52(5): 1501-1521. |
| [9] | 何万龙, 耿洪伟, 张飞飞, 米克热阿依·阿巴白克热, 罗紫洋, 李鹏程, 周钊宇, 程宇坤. 基于深度学习的小麦重要病害图像识别系统的研究[J]. 作物学报, 2026, 52(5): 1401-1417. |
| [10] | 张振, 冯连杰, 石玉, 于振文, 张永丽. 节水补灌下不同穗型小麦产量形成差异研究[J]. 作物学报, 2026, 52(5): 1522-1535. |
| [11] | 侯思宇, 王国璀, 韦金贵, 谢玮欣, 殷文, 樊志龙, 柴强, 胡发龙. 绿肥配施化学氮肥对西北干旱灌区小麦干物质积累及产量形成的影响[J]. 作物学报, 2026, 52(4): 1208-1219. |
| [12] | 尚云秋, 赵竹, 陈欢, 丁永刚, 乔玉强, 李玮, 张向前, 曹承富, 杜世州. 长期定位耕作方式对雨养小麦籽粒灌浆和产量形成的影响[J]. 作物学报, 2026, 52(4): 1236-1250. |
| [13] | 乔宇馨, 李程越, 康晓玉, 张鑫琪, 贾绍辉, 刘倩, 曹亚丽, 史鑫蕊, 郝兴宇, 李萍. 基于APSIM模型的长期免耕秸秆覆盖对旱地小麦增产效应研究[J]. 作物学报, 2026, 52(4): 1181-1192. |
| [14] | 李灿, 张喜伟, 朱博涛, 张沛沛. 小麦GSK激酶TaSK41的功能分析及互作蛋白的筛选[J]. 作物学报, 2026, 52(3): 677-687. |
| [15] | 侯洁, 付朵朵, 武海峰, 郝宇琼, 郑兴卫, 武棒棒, 周凯, 李晓华, 郑军, 赵佳佳. 山西省小麦地方品种的染色体多样性及遗传效应分析[J]. 作物学报, 2026, 52(3): 746-763. |
|
||