作物学报 ›› 2013, Vol. 39 ›› Issue (03): 423-430.doi: 10.3724/SP.J.1006.2013.00423
蔺芳芳,杨旭,武小翠,刘晓梅,葛荣朝,赵宝存*
LIN Fang-Fang,YANG Xu,WU Xiao-Cui,LIU Xiao-Mei,GE Rong-Chao,ZHAO Bao-Cun*
摘要:
TaSC (Triticum asetivum L. salt-tolerance related gene, GenBank 登录号为AY956330)是从小麦耐盐突变体RH8706-49 中克隆的高盐诱导表达的耐盐相关基因。以该基因编码的蛋白质TaSC 为诱饵, 运用分裂泛素酵母双杂交技术从小麦cDNA 表达文库中钓取互作蛋白质, 筛选到一个编码小麦未知功能蛋白质的基因(GenBank 登录号为AK336035), 命名为TaSCIP1 (TaSC interaction protein 1)。双分子荧光互补(BiFC)实验证实TaSCIP1 与TaSC 存在互作。该互作蛋白的分离有利于进一步研究TaSC 基因的耐盐机制。
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