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作物学报 ›› 2014, Vol. 40 ›› Issue (03): 416-423.doi: 10.3724/SP.J.1006.2014.00416

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

核盘菌诱导下甘蓝型油菜防御相关基因表达差异分析

马田田1,**,彭琦2,**,陈松2,张洁夫2,*   

  1. 1南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京 210095;2江苏省农业科学院经济作物研究所 / 农业部长江下游棉花与油菜重点实验室, 江苏南京 210014
  • 收稿日期:2013-07-02 修回日期:2013-10-28 出版日期:2014-03-12 网络出版日期:2014-01-17
  • 通讯作者: 张洁夫, E-mail: jiefu_z@163.com, Tel: 025-84390657
  • 基金资助:

    本研究由国家科技支撑计划项目(2010BAD01B10), 国家自然科学基金项目(31301357), 江苏省自然科学基金项目(BK20130719)和江苏省农业科技自主创新资金项目(CX(13)5009)资助。

Differential Expression of Defense Related Genes in Brassica napus Infected by Sclerotinia sclerotiorum

MA Tian-Tian1,**,PENG Qi2,**,CHEN Song2,ZHANG Jie-Fu2,*   

  1. 1 National Key Laboratory of Crop Genetics and Germplasm Innovation, Nanjing Agricultural University, Nanjing 210095, China; 2 Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture / Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2013-07-02 Revised:2013-10-28 Published:2014-03-12 Published online:2014-01-17
  • Contact: 张洁夫, E-mail: jiefu_z@163.com, Tel: 025-84390657

摘要:

菌核病是油菜主要病害, 至今尚未在油菜及相关植物中找到抗性基因。本研究利用qRT-PCR法比较了抗病品种宁RS-1和感病品种APL01在接种核盘菌后0~48 h11个防御相关基因的表达差异, 以揭示抗病品种宁RS-1的抗病机制。结果表明, 4个基因(PGIPCu/ZnSODOXOGLP)在核盘菌诱导前后抗、感品种内表达量均较高, 且抗病品种的表达量显著高于感病品种, 尤其是PGIP基因, 抗病品种宁RS-124 h的表达量为诱导前的170.4, 而感病品种仅为诱导前的3.5, 该时期抗病品种PGIP的表达量为感病品种的1299.4倍;2个基因(LOX2PDF1.2)在诱导前后抗、感品种内的表达量均较低, 抗、感品种间表达量差异显著;5个基因(FeSODPALEDS1PR1EIN3)诱导前后抗、感品种内的表达量均较低, 抗、感品种间的表达量差异不显著。推测抗病品种宁RS-1对菌核病的抗性可能是由于PGIP的上调表达, 抑制了核盘菌PG蛋白对侵染部位油菜组织细胞壁的降解, 从而抑制了油菜菌核病的发生与蔓延。

关键词: 核盘菌, 甘蓝型油菜, 防御基因, 表达差异

Abstract:

Sclerotinia stem rot is the main disease of rapeseed. Up to date, genes involved in defending Sclerotinia sclerotiorum have not been found in rapeseed and some other related plants. In order to reveal the disease resistance mechanism, we inoculated Sclerotinia sclerotiorum on the stems of resistant variety Ning RS-1 and susceptible one APL01, and compared eleven defensive related genes’ expression profiles between two varieties during the period of inoculation by using fluorescence quantitative PCR. Results showed that four genes (PGIP, Cu/ZnSOD, OXO, and GLP) were highly expressed both in Ning RS-1 and APL01, and the expression in Ning RS-1 was much higher than that in APL01. Especially, PGIP’s expression at 24 hours after inoculation (hai) with Sclerotinia sclerotiorum was 170.4 times as high as that at 0 hai in Ning RS-1, while it was only 3.5 times in APL01, and PGIP’s expression in Ning RS-1 was 1299.4 times as high as that at 24 hai in APL01. Two genes (LOX2 and PDF1.2)were expressed low both in Ning RS-1 and APL01, without significant difference between two varieties.Five genes (FeSOD, PAL, EDS1, PR1, and EIN3) were also expressed low in Ning RS-1 and APL01, without significant difference between two varieties. We inferred that the reason of resistance against Sclerotinia sclerotiorum in Ning RS-1 is related to the up-regulated expression of PGIP, which prevents the PGprotein in pathogen from degrading the cell wall of infected host tissues, resulting in the inhibition of incidence and spread of sclerotinia stem rot in rapeseed.

Key words: Sclerotinia sclerotiorum, Brassica napus, Defense gene, Differential expression

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