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作物学报 ›› 2014, Vol. 40 ›› Issue (08): 1380-1385.doi: 10.3724/SP.J.1006.2014.01380

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

豫粳6号B与TR2604间杂种花粉不育基因的定位

张宏根**,孙一标**,封智蔷,钱凯,裴艳,李闯,汤述翥*,梁国华,顾铭洪   

  1. 扬州大学农学院 / 江苏省作物遗传生理重点实验室 / 教育部植物功能基因组学重点实验室，江苏扬州225009
  • 收稿日期:2013-12-27 修回日期:2014-04-16 出版日期:2014-08-12 网络出版日期:2014-05-16
  • 通讯作者: 汤述翥, E-mail: sztang@yzu.edu.cn **同等贡献(Contributed equally to this work)
  • 基金资助:

    本研究由国家自然科学基金项目(31071384, 31000533)和江苏高校优势学科建设工程项目资助

Mapping of a Gene Causing Hybrid Pollen Sterility between Yujing 6B and TR2604

ZHANG Hong-Gen**,SUN Yi-Biao**,FENG Zhi-Qiang,QIAN Kai,PEI Yan,LI Chuang,TANG Shu-Zhu*,LIANG Guo-Hua,GU Ming-Hong   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • Received:2013-12-27 Revised:2014-04-16 Published:2014-08-12 Published online:2014-05-16
  • Contact: 汤述翥, E-mail: sztang@yzu.edu.cn **同等贡献(Contributed equally to this work)

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摘要：

阐明BT型杂交粳稻组合间育性差异的遗传基础有助于三系法杂交粳稻组合的选育。根据TR2604与豫粳6号A(B)、9201A(B)后代的花粉育性及小穗育性，明确了豫粳6号A(B)/TR2604 F1不育由双亲间特异性不亲和造成。遗传分析表明豫粳6号A(B)与TR2604 F1花粉不育受单基因S38(t)控制。以352株豫粳6号A/TR2604//TR2604、豫粳6号B/TR2604//豫粳6号B等群体中单株为定位群体，将S38(t)定位于第7染色体上标记RM18和RM234之间，与两标记遗传距离分别为0.43 cM和0.14 cM，两标记间物理距离为180 kb，相关结果为S38(t)图位克隆工作奠定了基础。

关键词: 三系杂交粳稻, 杂种花粉不育, 遗传分析, 基因定位

Abstract:

Understanding the genetic basis of spikelet fertility differences between japonica hybrids is good for BT-type three-line japonica hybrid breeding. According to the pollen and spikelet fertility of the plants in the populations derived from Yujing 6A(B), 9201A(B) and TR2604, it was confirmed that the sterility of Yujing 6A(B)/TR2604 F1 plants was induced by the incompatibility between the parents. Genetic analysis suggested that the hybrid pollen sterility character was controlled by a single nuclear gene named S38(t). A total of 352 plants from Yujing 6A//TR2604/TR2604 and Yujing 6B/TR2604//Yujing 6B segregation populations were used for gene mapping, and S38(t) wasmapped between RM18 and RM234 on chromosome 7 with genetic distances of 0.43 cM and 0.14 cM, respectively. The physical distance between RM18 and RM234 was about 118 kb. These results are useful for the map-based cloning of S38(t) gene.

Key words: Three-line japonica hybrid, Hybrid pollen sterility, Genetic analysis, Gene mapping

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