作物学报 ›› 2015, Vol. 41 ›› Issue (02): 228-239.doi: 10.3724/SP.J.1006.2015.00228
韩泽刚1,赵曾强1,何兰兰1,柴蒙亮2,李会会1,张薇1,*
HAN Ze-Gang1,ZHAO Zeng-Qiang1,HE Lan-Lan1,CHAI Meng-Liang2,LI Hui-Hui1,ZHANG Wei1,*
摘要:
以陆地棉抗病品种中棉所12和感病品种新陆早7号为材料, 利用Solexa高通量测序技术调查枯萎病菌诱导后不同时间感、抗陆地棉品种转录因子家族及转录因子的表达情况。结果表明, 枯萎病菌诱导后, 抗病品种中棉所12有39个转录因子家族的433个转录因子在至少一个比对组中表达活性发生变化; 感病品种新陆早7号则有52个转录因子家族的588个转录因子在至少一个比对组中表达活性发生变化。新陆早7号对枯萎病菌响应的转录因子及转录因子家族的数目明显多于中棉所12, 且2个品种的下调基因数目均多于上调基因。随着枯萎病菌诱导后时间延长, 两品种对枯萎病菌诱导响应的转录因子家族及转录因子数量均呈现先增加后降低的变化趋势, 中棉所12在枯萎病菌诱导后的6 h达最多, 而新陆早7号在诱导后的3 h达最多。在6个比对组中表达活性均发生变化的重叠转录因子, 中棉所12中有9个, 隶属于6个转录因子家族; 新陆早7号中有31个, 隶属于17个转录因子家族。不同抗病性品种对枯萎病的响应有较强的品种特异性, 除37个共有的转录因子家族外, 2个转录因子家族是中棉所12所特有, 15个转录因子家族是新陆早7号所特有。
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