作物学报 ›› 2017, Vol. 43 ›› Issue (01): 63-71.doi: 10.3724/SP.J.1006.2017.00063
白娜,李永祥*,焦付超,陈林,李春辉,张登峰,宋燕春,王天宇,黎裕,石云素*
BAI Na,LI Yong-Xiang*,JIAO Fu-Chao,CHEN Lin,LI Chun-Hui,ZHANG Deng-Feng,SONG Yan-Chun,WANG Tian-Yu,LI Yu,SHI Yun-Su*
摘要:
穗行数是影响玉米产量的重要因素之一,其遗传机制解析和关键基因精细定位对开展分子育种具有重要的意义。本研究以穗行数仅有4行的“四路糯选系”和多穗行自交系“农531”(18~22行)为亲本,构建了高代回交群体和次级定位群体(四路糯选系为供体亲本,农531为轮回亲本)。通过对不同类型试验群体的多环境表型鉴定和基因型鉴定,利用完备区间作图法(ICIM)进行穗行数主效QTL定位分析,将穗行数主效位点qKRN5.04定位到第5染色体136.3~140.0 Mb的区间之内;遗传效应分析发现,该位点在不同环境条件下最大可解释的表型变异为21.76%,效应值为0.80~1.76行。通过次级分离群体重组事件分析可将其进一步定位到~300 kb区间内。本研究结果不仅为分子标记辅助选择提供了实用的InDel标记,而且为玉米穗行数主效位点qKRN5.04的图位克隆和候选基因挖掘奠定了重要的基础。
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