作物学报 ›› 2017, Vol. 43 ›› Issue (06): 829-838.doi: 10.3724/SP.J.1006.2017.00829
董雪1,刘梦1,赵献林2,*,冯玉梅1,杨燕1,*
DONG Xue1,LIU Meng1,ZHAO Xian-Lin2,*,FENG Yu-Mei1,YANG Yan1,*
摘要:
低分子量麦谷蛋白约占小麦种子贮藏蛋白的三分之一,对面团延展性和食品加工品质有重要影响。普通小麦近缘种是小麦遗传改良的重要基因资源。本研究利用Glu-A3位点特异性引物对野生二粒小麦、栽培二粒小麦、硬粒小麦及野生一粒小麦共计9份材料进行GluA3-1、GluA3-2、GluA3-3基因扩增和鉴定,各发现5个等位变异,共计15个单元型;其中,有两个等位变异含有9个半胱氨酸残基,可能属于优良品质亚基。对近缘种中这些Glu-A3位点等位变异的鉴别,进一步完善了小麦低分子量麦谷蛋白亚基的构成,并为小麦品质育种中亲本的选择提供了相应依据。
[1] L?ve á. Conspectus of the Triticeae. Feddes Report, 1984, 95: 425–521 [2] Luo C, Griffin W B, Branlard G, Mcneil D L. Comparison of low- and high molecular-weight wheat glutenin allele effects on flour quality. Theor Appl Genet, 2001, 102: 1088–1098 [3] Zhen S M, Han C X, Ma C Y, Gu A Q, Zhang M, Shen X X, Li X H, Yan Y M. Deletion of the low-molecular-weight glutenin subunit allele Glu-A3a of wheat (Triticum aestivum L.) significantly reduces dough strength and breadmaking quality. BMC Plant Biol, 2014, 14: 1–17 [4] Mcintosh R A,Yamazaki Y, Devos K M, Dubcovsky J, Rogers W J, Appels R. Catalogue of gene symbols for wheat. Proceedings of the 10th international wheat genetics symposium, Paestum, Italy, 2003 [5] Liu C Y, Shepherd K W. Inheritance of B subunits of glutenin and ω-and γ-gliadins in tetraploid wheats. Theor Appl Genet, 1995, 90: 1149–1157 [6] Sreeramulu G, Singh N K. Genetic and biochemical characterization of novel low molecular weight glutenin subunits in wheat. Genome, 1997, 40: 41–48 [7] D'Ovidio R, Masci S. The low-molecular-weight glutenin subunits of wheat gluten. J Cereal Sci, 2004, 39: 321–339 [8] Luo G B, Zhang X F, Zhang Y L, Yang W L, Li Y W, Sun J Z, Zhan K H, Zhang A M, Liu D C. Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu. BMC Plant Biol, 2015, 15: 68 [9] 李黎, 李锁平, 李玉阁. 豫麦50低分子量麦谷蛋白亚基基因的克隆与序列分析. 麦类作物学报, 2015, 35: 1215–1221 Li L, Li S P, Li Y G. Cloning and sequence analysis of LMW-GS genes from common wheat (Triticum aestivum L.) cultivar Yumai 50. J Triticeae Crops, 2015, 35: 1215–1221 (in Chinese with English abstract) [10] Wang Y P, Zhen S M, Luo N N, Han C X, Lu X B, Li X H, Xia X C, He Z H, Yan Y M. Low molecular weight glutenin subunit gene Glu-B3h confers superior dough strength and breadmaking quality in wheat (Triticum aestivum L.). Sci Rep, 2016, 6: 27182 [11] Si H Q, Zhao M L, Zhang X, Yao Guo L, Sun G L, Ma C X. Cloning and characterization of low-molecular-weight glutenin subunit alleles from Chinese wheat landraces (Triticum aestivum L.). Sci World J, 2014, 2014: 371045 [12] Wang L H, Zhao X L, He Z H, Ma W, Appels R, Pe?a R J, Xia X C. Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theor Appl Genet, 2009, 118: 525–539 [13] Zhao X L, Yang Y, He Z H, Lei Z S, Ma W, Sun Q X, Xia X C. Characterization of novel LMW-GS genes at Glu-D3 locus of chromosome 1D in Aegilops tauschii. Hereditas, 2008, 145: 238–250 [14] 程西永, 吴少辉, 李海霞, 董中东, 任妍, 詹克慧, 许海霞. 小麦高、低分子量麦谷蛋白亚基对品质性状的影响. 麦类作物学报, 2014, 34: 482–488 Cheng X Y, Wu S H, Li H X, Dong Z D, Ren Y, Zhan K H, Xu H X. Effects of HMW and LMW glutenin subunits on wheat quality traits. J Triticeae Crops, 2014, 34: 482–488 (in Chinese with English abstract) [15] Ito M, Fushie S, Funatsuki W M, Ikeda T M, Nishio Z, Nagasawa K, Tabiki T, Yamauchi H. Effect of allelic variation in three glutenin loci on dough properties and bread-making qualities of winter wheat. Breed Sci, 2011, 61: 281–287 [16] Jin H, Zhang Y, Li G, Mu P, Fan Z, Xia X, He Z. Effects of allelic variation of HMW-GS and LMW-GS on mixograph properties and Chinese noodle and steamed bread qualities in a set of Aroona near-isogenic wheat lines. J Cereal Sci, 2013, 57: 146–152 [17] Zhang X, Jin H, Zhang Y, Liu D, Li G, Xia X, Zhang A. Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat. BMC Plant Biol, 2011, 12: 1–16 [18] Qin L M, Liang Y, Yang D Z, Sun L, Xia G G, Liu S W. Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in relation to Glu-3 evolution. Dev Genes Evol, 2015, 225: 31–37 [19] Andersen J R, Lübberstedt T. Functional markers in plants. Trends Plant Sci, 2003, 8: 554–560 [20] Zhang W, Gianibelli M C, Rampling L R, Gale K R. Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 108: 1409–1419 [21] 孙学永, 马传喜, 司红起, 张琪琪, 乔玉强. 中国小麦微核心种质低分子量麦谷蛋白Glu-A3位点等位基因的PCR检测. 分子植物育种, 2006, 4: 477–482 Sun X Y, Ma C X, Si H Q, Zhang Q Q, Qiao Y Q. Screening Glu-A3 alleles of LMW-GS genes from mini-core collections of Chinese wheat varieties using alleles-specific PCR markers. Mol Plant Breed, 2006, 4: 477–482 (in Chinese with English abstract) [22] 赵曼丽. 低分子量麦谷蛋白亚基基因的克隆及序列分析. 安徽农业大学硕士学位论文, 安徽合肥, 2014 Zhao M L. Cloning and sequence analysis of low molecular weight glutein subunit gene. MS Thesis of Anhui Agricultural University, Hefei, China, 2014 (in Chinese with English abstract) [23] 王林海. 普通小麦及其近缘种低分子量麦谷蛋白基因克隆与STS标记开发. 中国农业科学院博士学位论文, 北京, 2009 Wang L H. Characterisation of Low-Molecular-Weight Glutenin Genes and Development of STS Markers in Common Wheat and Its Relatives. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2009 (in Chinese with English abstract) [24] Gale K R, Ma W, Zhang W, Rampling L, Hill A S, Appels R, Morris P, Morrel M. Simple high-throughput DNA markers for genotyping in wheat. In: Eastwood R, Hollamby G, Rathjen T, Gororo N, eds. Proceedings of 10th Australian Wheat Breeding Assembly. Mildura VIC: Wheat Breeding Society of Australia, 2001. pp 26–31 [25] Stepien L, Holubec V. Chelkowski J. Resistance genes in wild accessions of Triticeae-inoculation test and STS marker analyses. Theor Appl Genet, 2002, 43: 423–435 [26] Qin L M, Liang Y, Yang D Z, Sun L, Xia G M, Liu S W. Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in relation to Glu-3 evolution. Dev Genes Evol, 2015, 225: 1–7 [27] Long H, Wei Y M, Yan Z H, Baum B, Nevo E, Zhen Y L. Classification of wheat low–molecular–weight glutenin subunit genes and its chromosome assignment by developing LMW-GS group-specific primers. Theor Appl Genet, 2005, 111: 1251–1259 [28] Zhao H X, Wang R J, Guo A G, Hu S W, Sun G L. Development of primers specific for LMW-GS genes located on chromosome 1D and molecular characterization of a gene from Glu-D3 complex locus in breed wheat. Hereditas, 2004, 141: 193–198 [29] Xu H, Wang R J, Shen X, Zhao Y L, Sun G L, Zhao H X, Guo A G. Functional properties of a new low -molecular -weight glutenin subunit gene from a bread wheat cultivar. Theor Appl Genet, 2006, 113: 1295–1303 |
[1] | 张钰坤, 陆赢, 崔看, 夏石头, 刘忠松. 芥菜种子颜色调控基因TT8的等位变异及其地理分布分析[J]. 作物学报, 2022, 48(6): 1325-1332. |
[2] | 黄义文, 代旭冉, 刘宏伟, 杨丽, 买春艳, 于立强, 于广军, 张宏军, 李洪杰, 周阳. 小麦多酚氧化酶基因Ppo-A1和Ppo-D1位点等位变异与穗发芽抗性的关系[J]. 作物学报, 2021, 47(11): 2080-2090. |
[3] | 张福彦, 程仲杰, 陈晓杰, 王嘉欢, 陈锋, 范家霖, 张建伟, 杨保安. 黄淮麦区小麦粒重基因等位变异的分子鉴定及育种应用[J]. 作物学报, 2021, 47(11): 2091-2098. |
[4] | 王娟,董承光,刘丽,孔宪辉,王旭文,余渝. 棉花适宜机采相关性状的SSR标记关联分析及优异等位基因挖掘[J]. 作物学报, 2017, 43(07): 954-966. |
[5] | 寇程,高欣,李立群,李扬,王中华,李学军*. 小麦粒重基因TaGW2-6A等位变异的组成分析及育种选择[J]. 作物学报, 2015, 41(11): 1640-1647. |
[6] | 李文,万千,刘风珍,张昆,张秀荣,厉广辉,万勇善. 花生转录因子基因NAC4的等位变异分析[J]. 作物学报, 2015, 41(01): 31-41. |
[7] | 相吉山,穆培源,桑伟,徐红军,聂迎彬,崔凤娟,庄丽,韩新年,邹波. 新疆小麦Psy-A1、Ppo-A1、Ppo-D1、TaLox-B1等位变异对面粉色泽的影响[J]. 作物学报, 2015, 41(01): 72-79. |
[8] | 张福彦,尚晓丽,吴培培,宋双,陈锋,崔党群. 硬粒小麦品种Lpx-B1位点等位变异的分子鉴定及其脂肪氧化酶活性[J]. 作物学报, 2014, 40(08): 1364-1370. |
[9] | 孙晓棠,卢冬冬,欧阳林娟,胡丽芳,边建民,彭小松,陈小荣,傅军如,贺晓鹏,贺浩华*,朱昌兰*. 水稻纹枯病抗性关联分析及抗性等位变异发掘[J]. 作物学报, 2014, 40(05): 779-787. |
[10] | 胡文明,阚海华,王伟,徐辰武. 等位基因功能差异的统计遗传学分析及应用[J]. 作物学报, 2014, 40(01): 72-79. |
[11] | 张国华,高明刚,张桂芝,孙金杰,靳雪梅,王春阳,赵岩,李斯深. 黄淮麦区小麦品种(系)产量性状与分子标记的关联分析[J]. 作物学报, 2013, 39(07): 1187-1199. |
[12] | 范虎,文自翔,王春娥,王芳,邢光南,赵团结,盖钧镒. 中国野生大豆群体农艺加工性状与SSR关联分析和特异材料的遗传构成[J]. 作物学报, 2013, 39(05): 775-788. |
[13] | 刘亚男,夏先春,何中虎. 普通小麦TaDep1基因克隆与特异性标记开发[J]. 作物学报, 2013, 39(04): 589-598. |
[14] | 黄冰艳,张新友,苗利娟,高伟,韩锁义,董文召,汤丰收,刘志勇. 花生ahFAD2A等位基因表达变异与种子油酸积累关系[J]. 作物学报, 2012, 38(10): 1752-1759. |
[15] | 武玉国,吴承来,秦保平,王振林,黄玮,杨敏,尹燕枰. 黄淮冬麦区175个小麦品种的SSR多态性及其与株高、产量相关性状的关联分析[J]. 作物学报, 2012, 38(06): 1018-1028. |
|