欢迎访问作物学报,今天是

作物学报 ›› 2017, Vol. 43 ›› Issue (10): 1426-1433.doi: 10.3724/SP.J.1006.2017.01426

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

一个水稻温敏黄化突变体的表型分析和基因定位

张天雨1,周春雷1,刘喜1,孙爱伶1,曹鹏辉1,Thanhliem NGUYEN1,田云录1,翟虎渠1,2,江玲1,*   

  1. 本研究由国家重点研发项目(2016YFD0100101-08),江苏省科技支撑计划项目(BE2015363)和安徽省科技重大专项(16030701068)资助。
  • 收稿日期:2017-03-02 修回日期:2017-05-10 出版日期:2017-10-12 网络出版日期:2017-05-23
  • 通讯作者: Jing Ling, E-mail:jiangling@njau.edu.cn
  • 基金资助:

    本研究由国家重点研发项目(2016YFD0100101-08),江苏省科技支撑计划项目(BE2015363)和安徽省科技重大专项(16030701068)资助。

Phenotypes and Gene Mapping of a Thermo-sensitive Yellow Leaf Mutant of Rice

ZHANG Tian-Yu1,ZHOU Chun-Lei1,LIU Xi1,SUN Ai-Ling1,CAO Peng-Hui1,Thanhliem NGUYEN1, TIAN Yun-Lu1,ZHAI Hu-Qu1,2,JIANG Ling1,*   

  1. This study was supported by the National Key Research and Development Program of China (2016YFD0100101-08), theScience and Technology Support Project of Jiangsu Province(BE2015363), and the Science and Technology Major Projects(16030701068).
  • Received:2017-03-02 Revised:2017-05-10 Published:2017-10-12 Published online:2017-05-23
  • Contact: Jing Ling, E-mail:jiangling@njau.edu.cn
  • Supported by:

    This study was supported by the National Key Research and Development Program of China (2016YFD0100101-08), theScience and Technology Support Project of Jiangsu Province(BE2015363), and the Science and Technology Major Projects(16030701068).

摘要:

对水稻叶色进行表型分析与基因定位,可为图位克隆该类基因和研究水稻光合系统功能奠定基础。利用甲基磺酸乙酯(Ethylmethylsulfone,EMS)诱变的方法,从籼稻品种“南京11”(简称NJ11)中获得温敏黄化突变体dy1;与野生型相比,dy1在自然环境下,从苗期至成熟期始终表现叶片黄化,透射电镜显示dy1类囊体结构异常;同时株高、分蘖数、结实率等农艺性状也表现出极显著的差异;实验室光照培养箱条件下,dy1苗期在20℃下表现白化,在25℃表现黄化,在30℃下为浅绿的表型;遗传分析表明水稻温敏黄化突变体dy1的表型是由1对隐性基因控制;将突变体与粳稻广亲和品种‘02428’杂交,构建F2群体,从中选出隐性极端个体,通过基因定位,将控制黄化的基因限定在第1染色体长臂上标记Y-4和Y-35之间的115 kb的区间内,含有16个开放阅读框(ORF),测序发现,dy1中编码尿嘧啶核苷酸激酶的基因LOC_Os01g73450的第4个内含子与第5个外显子交界处存在单碱基替换,拟作为候选基因;且叶绿体合成相关基因表达量显著降低,猜测该基因可能参与了叶绿素合成途径。

关键词: 水稻, 温度敏感型, 遗传分析, 叶色, 基因定位

Abstract:

Phenotypic analysis and gene mapping of rice leaf color could lay a foundation for map-based cloning of related genes and the function research of rice photosynthetic system. Leaf yellow mutant dy1 was obtained from rice cultivar “Nanjing 11”(abbreviated as NJ11) mutated by ethyl methanesulfonate (EMS). The mutant dy1 showed leaf yellowing at seedling stage and maturity stage in natural environment, with an abnormal structure of thylakoids under TEM, and significant differences in plant height, tiller number, seed setting rate and so on. The mutant dy1 showed albinism in 20°C,etiolation in 25°C andvirescence in 30°C.The rice leaf yellow mutant dy1 was controlled by a single recessive gene. F2 population was constructed by crossing the mutant with “02428”, and the mutant phenotypesof extreme individualswere selected to map gene dy1. The gene was located in the 115 kb region of the long arm of chromosome 1, and contained 16 ORFs. Sequencing analysis showed that LOC_Os01g73450controlling a uracil nucleotidekinase, with a single base substitution in the junction of the fourth intron and the fifth exon in dy1, might be a candidate gene.And the expression of genes relatedto chloroplast synthesis was significantly decreased, indicatingdy1 may involve in the chloroplast synthesis

Key words: Rice, Thermo-sensitive, Genetic analysis, Leaf color, Gene mapping

[1] Leister D. Chloroplast research in the genomic age. Trends Genet, 2003, 19: 47–56
[2] Afsar Awan M, Konzak C F, Rutger J N,Nilan R A. Mutagenic effects of sodium azide in rice. Crop Sci, 1980, 76: 663–668
[3] 胡忠, 彭丽萍, 蔡永华. 一个黄绿色的水稻细胞核突变体. 遗传学报, 1981,8:256–261
Hu Z, Peng L P, Cai Y H. A yellow rice mutants of nuclei. Acta Genet Sin, 1981, 8: 256–261(in Chinese)
[4] 陈青, 卢芙萍, 徐雪莲. 水稻叶色突变体研究进展. 热带生物学报, 2010, 1: 269–281
Chen Q, Lu F P, Xu X L. Progress in research of rice leaf coloration mutant. J Tropical Agric, 2010, 1: 269–281(in Chinese)
[5] Wu Z M, Zhang X, He B, Diao L P, Sheng S L, Wang J L, Guo X P, Su N, Wang L F, Jiang L, Wang C M, Zhai H Q, Wan J M. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29–40
[6] Yang Y L, Xu J, Huang L C, Leng Y J, Rao Y C, Chen L, Wang Q Q, Tu Z J, Hu J, Ren D Y, Zhang G H, Zhu L, Guo L B, Qian Q, Zeng D L. PGL, encoding chlorophyllide a oxygenase1, impacts leaf senescence and indirectly affects grain yield and quality in rice. J Exp Bot, 2007, 67: 297–310
[7] Zhang H T, Li J J, Yoo J H, Yoo S C, Cho S H, Koh H J, Seo H S, Peak N C. Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development. Plant Mol Biol, 2006, 62: 325–337
[8] Zhao C F, Xu J M, Chen Y, Mao C Z, Zhang S L, Bai Y H, Jiang D, Wu P. Molecular cloning and characterization of OsCHR4, a rice chromatin-remodeling factor required for early chloroplast development in adaxial mesophyll. Planta, 2012, 236: 1165–1176
[9] Yoo S C, Cho S H, Sugimoto H, Li J J, Kusumi K, Koh H J, Iba K, Peak N C. Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development. Plant Physiol, 2009, 150: 388–401
[10] Park S Y, Yu J W, Park J S, Li J J, Yoo S C, Lee S K, Lee N Y, Jeong S W, Jeong S W, Seo H S, Koh H J, Jeon J S, Park Y L, Peak N C. The Senescence-induced staygreen protein regulates chlorophyll degradation. Plant Cell, 2007, 19: 1649–1664
[11] Hartmut K, Lichtenthaler. Biosynthesis, accumulation and emission of carotenoids, a-tocopherol, plastoquinone,and isoprene in leaves under high photosynthetic irradiance. Photosynth Res, 2007, 92: 163–179
[12] 刘喜, 周春雷, 任雅琨, 扬春艳, 何旎清, 柳周, 江玲, 万建民. 水稻叶色白化转绿突变体WGL的遗传分析与基因定位. 南京农业大学学报, 2015, 38: 712–719
Liu X, Zhou C L, Ren Y K, Yang C Y, He N Q, Liu Z, Jiang L, Wan J M. Genetic analysis and gene mapping of virescent albino leaf mutant WGL in rice. JNanjing Agric Univ, 2015, 38:712–719(in Chinese with English abstract)
[13] 何旎清, 柳周, 张龙, 白苏阳, 田云录, 江玲, 万建民. 一个新的水稻黄绿叶突变体的遗传分析及突变基因的精细定位. 作物学报, 2015, 41: 1155–1163
He N Q, Liu Z, Zhang L,Bai S Y, Tian Y L, Jiang L, Wan J M. Genetic analysis of a new yellow-gren leaf mutant and fine-mapping of mutant gene in rice. Acta Agron Sin, 2015, 41: 1155–1163(in Chinese with English abstract)
[14] 孔飞, 蔡跃, 汪鹏, 尤小满, 张杰, 江玲, 张文伟, 万建民. 水稻温度敏感型黄叶突变体yl2(t)的表型分析和基因定位. 南京农业大学学报, 2016, 39: 703-710
Kong F, Cai Y, Wang P, You X M, Zhang J, Jiang L, Zhang W W, Wan J M. Characterization and gene mapping of a thermo-sensitive yellow leaf mutant yl2(t)in rice. J Nanjing Agric Univ, 2016, 39: 703–710 (in Chinese with English abstract)
[15] 刘艳霞, 林冬枝, 董彦君. 水稻温敏感叶色突变体研究进展. 中国水稻科学, 2015,29: 439–446
Liu Y X, Lin D Z, Dong Y J. Research advances in thermo-sensitive leaf coloration mutants in rice. Chin J Rice Sci, 2015,29: 439–446 (in Chinese with English abstract)
[16] Kensuke K, Shoko H, Hiroshi S, Yoko C, Osanu M, Koh I. Contribution of chloroplast biogenesis to carbon-nitrogen balance during early leaf development in rice. J Plant Res, 2010, 123: 617–622
[17] Hiroki S, Kensuke K, Ko N, Masahiro Y, Atsushi Y, Koh I. The rice nuclear gene, VIRESCENT 2, is essential for chloroplast development and encodes a novel type of guanylate kinase targeted to plastids and mitochondria. Plant J, 2007, 52: 512–527
[18] Gong X D, Su Q Q, Lin D Z, Jiang Q, Xu J L, Zhang J H, Teng S, Dong Y J. The rice OsV4 encoding a novel pentatricopeptide repeat protein is required for chloroplast development during the early leaf stage under cold stress. J Integr Plant Biol, 2014, 56: 400–410
[19] Jiang Q, Mei J, Gong X D, Xu J L, Zhang J H, Teng S, Lin D Z, Dong Y J. Importance of the rice TCD9 encoding subunit of chaperonin protein 60 (Cpn60) for the chloroplast development during the early leaf stage. Plant Sci, 2014, 215-216: 172–179
[20] Song J, Wei X J, Shao G N, Sheng Z H, Chen D B, Liu C L, Jiao G A, Xie L L, Tang S Q, Hu P S. The rice nuclear gene WLP1 encoding a chloroplast ribosome L13 protein is needed for chloroplast development in rice grown under low temperature conditions.Plant Mol Biol, 2014, 84: 301–314
[21] Kensuke K, Chikako S, Takahiro N, Shinji K, Atsushi Y, Koh I. A plastid protein NUS1 is essential for build-up of the genetic system for early chloroplast development under cold stress conditions. Plant J, 2011, 68:1039–1050
[22] Toda T, Fuji S, Noguchi K, Tomohiko K, Kinya T. Rice MPR25 encodes a pentatricopeptide repeat protein and is essential for RNA editing of nad5 transcripts in mitochondria. Plant J, 2012, 72: 450–460
[23] Peng L W, Fukao Y, Myouga F, Motohashi R, Shinozaki K, Shikanai T. A chaperonin subunit with unique structures is essential for folding of a specific substrate. PLOS Biol, 2011, 9: 1–13
[24] Zhou L, Lacroute F, Thornburg R. Cloning, expression in Escherichia coli, and characterization of Arabidopsis thaliana UMP/CMP kinase. Plant Physiol, 1998, 117: 245–254
[25] Hein P, Stockel J, Bennewitz S, Oelmuller R. A protein related to prokaryotic UMP kinases is involved in psaA/B transcript accumulation in Arabidopsis. Plant Mol Biol, 2009, 69: 517–528

[1] 田甜, 陈丽娟, 何华勤. 基于Meta-QTL和RNA-seq的整合分析挖掘水稻抗稻瘟病候选基因[J]. 作物学报, 2022, 48(6): 1372-1388.
[2] 郑崇珂, 周冠华, 牛淑琳, 和亚男, 孙伟, 谢先芝. 水稻早衰突变体esl-H5的表型鉴定与基因定位[J]. 作物学报, 2022, 48(6): 1389-1400.
[3] 周文期, 强晓霞, 王森, 江静雯, 卫万荣. 水稻OsLPL2/PIR基因抗旱耐盐机制研究[J]. 作物学报, 2022, 48(6): 1401-1415.
[4] 郑小龙, 周菁清, 白杨, 邵雅芳, 章林平, 胡培松, 魏祥进. 粳稻不同穗部籽粒的淀粉与垩白品质差异及分子机制[J]. 作物学报, 2022, 48(6): 1425-1436.
[5] 颜佳倩, 顾逸彪, 薛张逸, 周天阳, 葛芊芊, 张耗, 刘立军, 王志琴, 顾骏飞, 杨建昌, 周振玲, 徐大勇. 耐盐性不同水稻品种对盐胁迫的响应差异及其机制[J]. 作物学报, 2022, 48(6): 1463-1475.
[6] 杨建昌, 李超卿, 江贻. 稻米氨基酸含量和组分及其调控[J]. 作物学报, 2022, 48(5): 1037-1050.
[7] 杨德卫, 王勋, 郑星星, 项信权, 崔海涛, 李生平, 唐定中. OsSAMS1在水稻稻瘟病抗性中的功能研究[J]. 作物学报, 2022, 48(5): 1119-1128.
[8] 朱峥, 王田幸子, 陈悦, 刘玉晴, 燕高伟, 徐珊, 马金姣, 窦世娟, 李莉云, 刘国振. 水稻转录因子WRKY68在Xa21介导的抗白叶枯病反应中发挥正调控作用[J]. 作物学报, 2022, 48(5): 1129-1140.
[9] 王小雷, 李炜星, 欧阳林娟, 徐杰, 陈小荣, 边建民, 胡丽芳, 彭小松, 贺晓鹏, 傅军如, 周大虎, 贺浩华, 孙晓棠, 朱昌兰. 基于染色体片段置换系群体检测水稻株型性状QTL[J]. 作物学报, 2022, 48(5): 1141-1151.
[10] 王泽, 周钦阳, 刘聪, 穆悦, 郭威, 丁艳锋, 二宫正士. 基于无人机和地面图像的田间水稻冠层参数估测与评价[J]. 作物学报, 2022, 48(5): 1248-1261.
[11] 陈悦, 孙明哲, 贾博为, 冷月, 孙晓丽. 水稻AP2/ERF转录因子参与逆境胁迫应答的分子机制研究进展[J]. 作物学报, 2022, 48(4): 781-790.
[12] 王好让, 张勇, 于春淼, 董全中, 李微微, 胡凯凤, 张明明, 薛红, 杨梦平, 宋继玲, 王磊, 杨兴勇, 邱丽娟. 大豆突变体ygl2黄绿叶基因的精细定位[J]. 作物学报, 2022, 48(4): 791-800.
[13] 刘磊, 詹为民, 丁武思, 刘通, 崔连花, 姜良良, 张艳培, 杨建平. 玉米矮化突变体gad39的遗传分析与分子鉴定[J]. 作物学报, 2022, 48(4): 886-895.
[14] 王吕, 崔月贞, 吴玉红, 郝兴顺, 张春辉, 王俊义, 刘怡欣, 李小刚, 秦宇航. 绿肥稻秆协同还田下氮肥减量的增产和培肥短期效应[J]. 作物学报, 2022, 48(4): 952-961.
[15] 巫燕飞, 胡琴, 周棋, 杜雪竹, 盛锋. 水稻延伸因子复合体家族基因鉴定及非生物胁迫诱导表达模式分析[J]. 作物学报, 2022, 48(3): 644-655.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!