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作物学报 ›› 2018, Vol. 44 ›› Issue (02): 159-168.doi: 10.3724/SP.J.1006.2018.00159

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

利用SSR和SNP标记分析鲁麦14对青农2号的遗传贡献

李玉刚1,**, 任民2,**, 孙绿1,3, 王圣健1, 韩梅1,3, 李振清1, 翟晓灵1, 代小雁4, 侯元江4,*(), 盖红梅1,*()   

  1. 1青岛市农业科学研究院, 山东青岛, 266100
    2中国农业科学院烟草研究所 / 烟草遗传改良与生物技术重点开放实验室, 山东青岛 266101
    3青岛农业大学 / 山东省旱作农业技术重点实验室, 山东青岛 266109
    4山东省青丰种子有限公司, 山东青岛 266700
  • 收稿日期:2017-04-10 接受日期:2017-11-21 出版日期:2018-02-12 网络出版日期:2017-12-12
  • 通讯作者: 李玉刚,任民,侯元江,盖红梅
  • 作者简介:liyg1967@163.com; ** 同等贡献(Contributed equally to this work)
  • 基金资助:
    本研究由国家重点研发计划项目(2016YFD0100300), 国家自然科学基金项目(31301320), 山东省农业良种工程项目(2016LZ01-01)和青岛市现代农业产业技术体系小麦产业创新推广团队资助

Genetic Contribution of Lumai 14 to Qingnong 2 Revealed by SSR and SNP Markers

Yu-Gang LI1,**, Min REN2,**, Lv SUN1,3, Sheng-Jian WANG1, Mei HAN1,3, Zhen-Qing LI1, Xiao-Ling ZHAI1, Xiao-Yan DAI4, Yuan-Jiang HOU4,*(), Hong-Mei GE1,*()   

  1. 1 Qingdao Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
    2 Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China
    3 College of Agronomy and Plant Protection, Qingdao Agricultural University / Shandong Provincial Key Laboratory of Dryland Farming Techniques, Qingdao 266109, Shandong, China
    4 Shandong Qingfeng Seed Company limited, Qingdao 266700, Shandong, China
  • Received:2017-04-10 Accepted:2017-11-21 Published:2018-02-12 Published online:2017-12-12
  • Contact: Yu-Gang LI,Min REN,Yuan-Jiang HOU,Hong-Mei GE
  • Supported by:
    This study was supported by the National Key Research and Development Program of China (2016YFD0100300), the National Natural Science Foundation of China (31301320), the Breeding Improvement Project of Shandong Province (2014LZ01-01), and the Wheat Industry Innovative Team of Qingdao Agriculture Research System.

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摘要：

鲁麦14既是大面积推广品种, 又是育种骨干亲本, 衍生了40多个品种, 其中青农2号(鲁麦14/烟农15//矮秆麦)是近年审定的小麦品种。本研究利用350个SSR标记和小麦90k芯片检测的26 026个SNP标记, 解析了鲁麦14对青农2号的遗传贡献。鲁麦14和烟农15分别含有1/4和1/2蚰包麦血统, 基因组分子标记分析结果显示, 这两个品种有55.42%的SSR位点一致, 而SNP位点一致性高达71.53%。选择亲本间差异位点, 分析鲁麦14和烟农15对青农2号的遗传贡献, 结果表明鲁麦14对青农2号的贡献大于烟农15, 青农2号与亲本鲁麦14、烟农15分子标记的一致性, SSR标记分别为54.11%和36.30%, SNP标记分别为72.55%和26.98%。依据高通量SNP标记结果, 从染色体水平看, 烟农15贡献率超过50%的染色体有2B、3B和6A; 而鲁麦14在除此之外的18条染色体的遗传贡献率大于50%。青农2号遗传组成图谱揭示了遗传物质多以较大染色体片段形式从亲本传递至子代。对亲本和子代进行多年多点的农艺性状调查, 发现青农2号的旗叶长、旗叶宽、穗下节间长、穗叶距、抽穗度等株型相关性状及千粒重、粒长等产量相关性状与鲁麦14相近, 株高、生育期等性状与烟农15相近。本文从分子层面解析育种亲本对子代的遗传贡献, 为分子标记辅助育种提供了依据和理论基础。

关键词: 骨干亲本, 遗传贡献, 遗传组成, SSR, 90k芯片

Abstract:

Lumai 14, a widely planted wheat variety, is the parents of more than 40 bread wheat varieties, among which Qingnong 2 (Lumai 14/Yannong 15//Aiganmai) has been released in recent years. In this study, the inheritance from Lumai 14 to Qingnong 2 was evaluated using 350 SSR markers and 26 026 SNPs detected by iSelect 90k SNP array. Pedigrees show that Lumai 14 and Yannong 15 contain 1/4 and 1/2 blood of Youbaomai, respectively. Genomic marker screening confirmed that Lumai 14 and Yannong 15 shared 55.42% common SSR loci and 71.53% common SNP loci. Polymorphic markers between them were used to disclose that Qingnong 2 inherited more SSR and SNP loci from Lumai 14 (54.11% and 72.55%, respectively) than from Yannong 15 (36.30% and 26.98%, respectively). In Qingnong 2, chromosomes 2B, 3B, and 6A harbored more than 50% SNPs from Yannong 15, whereas, the remaining 18 chromosomes carried more than 50% SNPs from Lumai 14. The parental genetic compositions were present mainly in large chromosomal segments in Qingnong 2. Phenotyping investigation in multi-locations across years indicated that Qingnong 2 was similar to Lumai 14 in length and width of flag leaf, length of first internode, length from flag leaf pulvinus to spike base, earing degree, thousand-grain weight, and grain length, and similar to Yanong 15 in plant height and growth duration. This results provide valuable information for molecular marker-assisted selection in wheat breeding.

Key words: founder parents, genetic contribution, genetic composition, SSR, 90k array

表1

性状调查的年份和地点"

年份
Year		 地点
Location		 环境数
Number of environments
2013-2014 山东青岛城阳, 山东泰安肥城, 河南新乡 3
2014-2015 山东青岛城阳1), 山东青岛平度, 山东泰安肥城, 河南新乡 4
2015-2016 山东青岛城阳1), 山东青岛平度, 山东泰安肥城 3

图1

青农2号亲本鲁麦14(A)和烟农15(B)的系谱该系谱根据方正等[33]报道整理。"

图2

鲁麦14和烟农15 SSR (a)和SNP位点(b)的异同比例和染色体组分布"

图3

用SSR和SNP标记分析鲁麦14和烟农15对青农2号的贡献(a) 全基因组水平; (b) SSR标记在不同染色体的分布; (c) SNP标记在不同染色体的分布。"

表2

鲁麦14和烟农15在21条染色体对青农2号的遗传贡献"

染色体
Chr.		 SSR SNP
差异位点 Differential loci 鲁麦14
Lumai 14		 贡献率 Contribution (%) 烟农15
Yannong 15		 贡献率 Contribution (%) 差异位点 Differential loci 鲁麦14
Lumai 14		 贡献率 Contribution (%) 烟农15
Yannong 15		 贡献率 Contribution (%)
1A 11 5 45.45 4 36.36 660 433 65.61 227 34.39
1B 10 6 60.00 3 30.00 685 620 90.51 65 9.49
1D 10 8 80.00 1 10.00 145 140 96.55 5 3.45
2A 9 4 44.44 3 33.33 270 238 88.15 32 11.85
2B 6 3 50.00 3 50.00 302 80 26.49 220 72.85
2D 8 3 37.50 4 50.00 325 321 98.77 3 0.92
染色体
Chr.		 SSR SNP
差异位点 Differential loci 鲁麦14
Lumai 14		 贡献率 Contribution (%) 烟农15
Yannong 15		 贡献率 Contribution (%) 差异位点 Differential loci 鲁麦14
Lumai 14		 贡献率 Contribution (%) 烟农15
Yannong 15		 贡献率 Contribution (%)
3A 7 5 71.43 1 14.29 362 318 87.85 43 11.88
3B 4 1 25.00 3 75.00 202 51 25.25 150 74.26
3D 3 1 33.33 2 66.67 66 65 98.48 1 1.52
4A 3 2 66.67 1 33.33 173 155 89.60 18 10.40
4B 7 1 14.29 5 71.43 134 73 54.48 58 43.28
4D 3 2 66.67 1 33.33 15 14 93.33 1 6.67
5A 5 3 60.00 2 40.00 319 277 86.83 42 13.17
5B 5 2 40.00 3 60.00 233 151 64.81 81 34.76
5D 18 10 55.56 7 38.89 44 33 75.00 10 22.73
6A 9 4 44.44 5 55.56 390 121 31.03 263 67.44
6B 6 4 66.67 2 33.33 511 393 76.91 117 22.90
6D 3 2 66.67 1 33.33 77 68 88.31 6 7.79
7A 4 3 75.00 0 0.00 369 286 77.51 83 22.49
7B 6 5 83.33 0 0.00 607 433 71.33 166 27.35
7D 9 5 55.56 2 22.22 35 28 80.00 7 20.00
Total 146 79 54.11 53 36.30 5924 4298 72.55 1598 26.98

图4

青农2号的遗传组成图谱每组图谱前3根表示染色体, 自左向右依次代表鲁麦14、烟农15和青农2号, 第4根表示SNP在整合遗传图中的染色体定位。红色、蓝色、浅黄色、灰色分别表示鲁麦14特有、烟农15特有、共有、非亲本等位变异。"

表3

青农2号及其亲本鲁麦12、烟农15在多环境下农艺性的无偏估计（BLUP）值"

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