欢迎访问作物学报，今天是
作物学报 ›› 2018, Vol. 44 ›› Issue (04): 473-482.doi: 10.3724/SP.J.1006.2018.00473
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
朱展望1,2(
), 徐登安1, 程顺和3,*(), 高春保2, 夏先春1, 郝元峰1,*(), 何中虎1,4
Zhan-Wang ZHU1,2(), Deng-An XU1, Shun-He CHENG3,*(), Chun-Bao GAO2, Xian-Chun XIA1, Yuan-Feng HAO1,*(), Zhong-Hu HE1,4
摘要：
提高赤霉病抗性已成为我国小麦主产区的重要育种目标之一。Fhb1是抗性最强且最稳定的抗赤霉病基因, 阐明其在我国小麦育种中的应用及传递路径, 对抗赤霉病育种有重要意义。本研究通过分析229份小麦品种(系) Fhb1区段内PFT (pore-forming toxin-like)、HC (HCBT-like defense response protein)和His (histidine-rich calcium-binding protein)基因的多样性与赤霉病抗性的关系, 发现PFT-I/His-I为抗病单倍型。基因检测和系谱分析表明, 中国小麦品种所含Fhb1至少有2个来源, 分别为苏麦3号和宁麦9号, 并以后者为主。本研究开发的诊断性标记PFT-CAPS和His-InDel可有效用于Fhb1的分子标记辅助育种。
| [1] | Dexter J E, Clear R M, Preston K R.Fusarium head blight: effect on the milling and baking of some Canadian wheats.Cereal Chem, 1996, 73: 695-701 |
| [2] | Dexter J E, Marchylo B A, Clear R M, Clarke J M.Effect of Fusarium head blight on semolina milling and pasta-making quality of durum wheat.Cereal Chem, 1997, 74: 519-525 |
| [3] | 程顺和, 郭文善, 王龙俊, 姜东, 马鸿翔, 张伯桥. 中国南方小麦. 南京: 江苏科学技术出版社, 2012. pp 281-282 |
| Cheng S H, Guo W S, Wang L J, Jiang D, Ma H X, Zhang B Q.Wheat in Southern China. Nanjing: Jiangsu Scientific and Technical Publishers, 2012. pp 281-282 (in Chinese) | |
| [4] | 马忠华, 尹燕妮, 陈云. 小麦赤霉病发生规律及其防控技术研究进展. 见: 陈万权主编. 植保科技创新与农业精准扶贫—中国植物保护学会2016年学术年会论文集. 北京: 中国农业科学技术出版社, 2016. p 29 |
| Ma Z H, Yin Y N, Chen Y.The epidemic characteristics of wheat Fusarium head blight and the progress of its control technology development. In: Chen W Q, ed. Plant Protection Technology Innovation and Agricultural Targeted Poverty Alleviation— Proceedings of the 2016 Annual Conference of China Plant Protection Society. Beijing: China Agricultural Science and Technology Press, 2016. p 29 (in Chinese) | |
| [5] | 吴佳文, 杨荣明, 朱凤, 田子华. 2015年江苏省小麦赤霉病发生特点与防控对策探讨. 中国植保导刊, 2016, 36(10): 31-34 |
| Wu J W, Yang R M, Zhu F, Tian Z H.The epidemic characteristics of wheatFusarium head blight in Jiangsu Province in 2015 and discussion of its control measures. China Plant Prot, 2016, 36(10): 31-34 (in Chinese) | |
| [6] | 金艳, 刘付领, 朱统泉, 陈杰, 赵立尚. 河南省小麦赤霉病的发生情况分析与防治对策. 河南科技学院学报(自然科学版), 2016, 44(6): 1-4 |
| Jin Y, Liu F L, Zhu T Q, Chen J, Zhao L S.Occurrence analysis and control measures of wheat scab in Henan province.J Henan Inst Sci & Technol(Nat Sci Edn), 2016, 44(6): 1-4 (in Chinese with English abstract) | |
| [7] | McMullen M, Jones R, Gallenberg D. Scab of wheat and barley: a re-emerging disease of devastating impact.Plant Dis, 1997, 81: 1340-1348 |
| [8] | Buerstmayr H, Ban T, Anderson J A.QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review.Plant Breed, 2009, 128: 1-26 |
| [9] | Cuthbert P A, Somers D J, Thomas J, Cloutier S, Brulé-Babel A.Fine mapping Fhb1, a major gene controlling Fusarium head blight resistance in bread wheat(Triticum aestivum L.). Theor Appl Genet, 2006, 112: 1465-1472 |
| [10] | Cuthbert P A, Somers D J, Brulé-Babel A.Mapping ofFhb2 on chromosome 6BS: a gene controlling Fusarium head blight field resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2007, 114: 429-437 |
| [11] | Liu S, Zhang X, Pumphrey M O, Stack R W, Gill B S, Anderson J A.Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance to Fusarium head blight in wheat.Funct Integr Genomics, 2006, 6: 83-89 |
| [12] | Qi L, Pumphrey M, Friebe B, Chen P, Gill B.Molecular cytogenetic characterization of alien introgressions with gene Fhb3 for resistance to Fusarium head blight disease of wheat. Theor Appl Genet, 2008, 117: 1155-1166 |
| [13] | Xue S, Li G, Jia H, Xu F, Lin F, Tang M, Wang Y, An X, Xu H, Zhang L.Fine mapping Fhb4 , a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). L. , 2010, 121: 147-156 |
| [14] | Xue S, Xu F, Tang M, Zhou Y, Li G, An X, Lin F, Xu H, Jia H, Zhang L.Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat(Triticum aestivum L.). Theor Appl Genet, 2011, 123: 1055-1063 |
| [15] | Cainong J C, Bockus W W, Feng Y, Chen P, Qi L, Sehgal S K, Danilova T V, Koo D H, Friebe B, Gill B S.Chromosome engineering, mapping, and transferring of resistance to Fusarium head blight disease from Elymus tsukushiensis into wheat. Theor Appl Genet, 2015, 128: 1019-1027 |
| [16] | Guo J, Zhang X, Hou Y, Cai J, Shen X, Zhou T, Xu H, Ohm H W, Wang H, Li A, Han F, Wang H, Kong L.High-density mapping of the major FHB resistance geneFhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection. Theor Appl Genet, 2015, 128: 2301-2316 |
| [17] | Schweiger W, Steiner B, Vautrin S, Nussbaumer T, Siegwart G, Zamini M, Jungreithmeier F, Gratl V, Lemmens M, Mayer K F X, Bérgès H, Adam G, Buerstmayr H. Suppressed recombination and unique candidate genes in the divergent haplotype encoding Fhb1, a major Fusarium head blight resistance locus in wheat. Theor Appl Genet, 2016, 129: 1607-1623 |
| [18] | Pumphrey M O, Bernardo R, Anderson J A.Validating the Fhb1 QTL for Fusarium head blight resistance in near-isogenic wheat lines developed from breeding populations. Crop Sci, 2007, 47: 200-206 |
| [19] | Horevaj P, Brown-Guedira G, Milus E A.Resistance in winter wheat lines to deoxynivalenol applied into florets at flowering stage and tolerance to phytotoxic effects on yield.Plant Pathol, 2012, 61: 925-933 |
| [20] | Mergoum M, Frohberg R C, Stack R W.Breeding hard red spring wheat for Fusarium head blight resistance. In: Buck H T, Nisi J E, Salomón N, eds. Wheat Production in Stressed Environments: Proceedings of the 7th International Wheat Conference. Dordrecht, Netherlands: Springer, 2007. pp 161-167 |
| [21] | Anderson J A, Wiersma J J, Linkert G L, Kolmer J A, Jin Y, Dillmacky R, Wiersma J V, Hareland G A.Registration of ‘Sabin’ Wheat.J Plant Reg, 2012, 6: 174 |
| [22] | Steiner B, Buerstmayr M, Michel S, Schweiger W, Lemmens M, Buerstmayr H.Breeding strategies and advances in line selection for Fusarium head blight resistance in wheat. Trop Plant Pathol, 2017, 42: 165-174 |
| [23] | Bai G, Shaner G.Management and resistance in wheat and barley to Fusarium head blight.Annu Rev Phytopathol, 2004, 42: 135-161 |
| [24] | Rawat N, Pumphrey M O, Liu S, Zhang X, Tiwari V K, Ando K, Trick H N, Bockus W W, Akhunov E, Anderson J A, Gill B S.Wheat Fhb1 encodes a chimeric lectin with agglutinin domains and a pore-forming toxin-like domain conferring resistance to Fusarium head blight. Nat Genet, 2016, 48: 1576-1580 |
| [25] | He X, Bonnett D, Singh P K, Hyles J, Spielmeyer W, Dreisigacker S.Advanced wheat breeding lines combining Fhb1 and Sr2 in different genetic backgrounds. In: Proceedings of the 9th International Wheat Conference. Sydney, Australia, 2015. p 140 |
| [26] | 孙道杰, 张玲丽, 冯毅, 陈春环, 张荣琦, 奚亚军, 何心尧, 王辉, 宋哲民. 西农系列小麦骨干新品种赤霉病抗源浅析. 麦类作物学报, 2016, 36: 822-823 |
| Sun D J, Zhang L L, Feng Y, Chen C H, Zhang R Q, Xi Y J, He X Y, Wang H, Song Z M.Analysis of FHB resistance sources for newly released Xinong varieties.J Triticeae Crops, 2016, 36: 822-823 (in Chinese) | |
| [27] | 朱展望, 朱伟伟, 佟汉文, 刘易科, 陈泠, 杨立军, 汪华, 张宇庆, 邹娟, 焦春海, 高春保. 湖北小麦品种赤霉病抗性及其在生产中的应用. 麦类作物学报, 2015, 35: 1733-1738 |
| Zhu Z W, Zhu W W, Tong H W, Liu Y K, Chen L, Yang L J, Wang H, Zhang Y Q, Zou J, Jiao C H, Gao C B.Fusarium head bight resistance and application of wheat varieties in Hubei Province.J Triticeae Crops, 2015, 35: 1733-1738 (in Chinese with English abstract) | |
| [28] | 朱展望, 杨立军, 佟汉文, 唐道廷, 刘易科, 汪华, 陈泠, 张宇庆, 高春保. 湖北省小麦品种(系)的赤霉病抗性分析. 麦类作物学报, 2014, 34: 137-142 |
| Zhu Z W, Yang L J, Tong H W, Tang D T, Liu Y K, Wang H, Chen L, Zhang Y Q, Gao C B.Analysis of the resistance to Fusarium head blight in wheat varieties and lines from Hubei.J Triticeae Crops, 2014, 34: 137-142 (in Chinese with English abstract) | |
| [29] | Stack R W, McMullen M P. A visual scale to estimate severity of Fusarium head blight in Wheat. North Dakota State University Extension Service 1994. p 1095 |
| [30] | Wang J, Li H, Zhang L, Meng L.Users’ Manual of QTL IciMapping. The Quantitative Genetics Group, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), and Genetic Resources Program, International Maize and Wheat Improvement Center (CIMMYT), 2016 |
| [31] | Saghai-Maroof M A, Soliman K M, Jorgensen R A, Allard R W. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics.Proc Natl Acad Sci USA, 1984, 81: 8014-8018 |
| [32] | Anderson J A, Chao S, Liu S.Molecular breeding using a major QTL for Fusarium head blight resistance in wheat.Crop Sci, 2007, 47: S112-S119 |
| [33] | Anderson J A, Stack R W, Liu S, Waldron B L, Fjeld A D, Coyne C, Moreno-Sevilla B, Fetch J M, Song Q J, Cregan P B, Frohberg R C.DNA markers for Fusarium head blight resistance QTLs in two wheat populations.Theor Appl Genet, 2001, 102: 1164-1168 |
| [34] | Liu S, Pumphrey M O, Gill B S, Trick H N, Zhang J X, Dolezel J, Chalhoub B, Anderson J A.Toward positional cloning of Fhb1, a major QTL for Fusarium head blight resistance in wheat. Cereal Res Commun, 2008, 36: 195-201 |
| [35] | Jin F, Zhang D, Bockus W, Baenziger P S, Carver B, Bai G.Fusarium head blight resistance in U.S. Winter wheat cultivars and elite breeding lines. Crop Sci, 2012, 53: 2006-2013 |
| [36] | Bernardo A N, Ma H, Zhang D, Bai G.Single nucleotide polymorphism in wheat chromosome region harboring Fhb1 for Fusarium head blight resistance. Mol Breed, 2012, 29: 477-488 |
| [37] | Buerstmayr H, Steiner B, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schneider B, Lemmens M.Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat: II. Resistance to fungal penetration and spread.Theor Appl Genet, 2003, 107: 503-508 |
| [38] | Chen J, Griffey C A, Saghai Maroof M A, Stromberg E L, Biyashev R M, Zhao W, Chappell M R, Pridgen T H, Dong Y, Zeng Z. Validation of two major quantitative trait loci for fusarium head blight resistance in Chinese wheat line W14.Plant Breed, 2006, 125: 99-101 |
| [39] | 金善宝. 中国小麦品种志(1983-1993). 北京: 中国农业出版社, 1997. pp 249-250 |
| Jin S B. Chinese Wheat Varieties(1983-1993). Beijing: China Agriculture Press, 1997. pp 249-250 (in Chinese) | |
| [40] | Li T, Bai G, Wu S, Gu S.Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu.Euphytica, 2012, 185: 93-102 |
| [41] | Yu J B, Bai G H, Cai S B, Ban T.Marker-assisted characterization of Asian wheat lines for resistance to Fusarium head blight.Theor Appl Genet, 2006, 113: 308-320 |
| [42] | 杨学明, 姚金保, 钱存鸣, 周朝飞, 姚国才, 黄胜东. 宁麦9号选育方法的探讨. 麦类作物学报, 2000, 20: 88-90 |
| Yang X M, Yao J B, Qian C M, Zhou C F, Yao G C, Huang S D.Discussion on the breeding method of Ningmai 9.J Triticeae Crops, 2000, 20: 88-90 (in Chinese) | |
| [43] | 姚金保, 马鸿翔, 张平平, 姚国才, 杨学明, 任丽娟, 张鹏, 周淼平. 小麦优良亲本宁麦9号的研究与利用. 核农学报, 2012, 26: 17-21 |
| Yao J B, Ma H X, Zhang P P, Yao G C, Yang X M, Ren L J, Zhang P, Zhou M P.Research of wheat elite parent Ningmai 9 and its utilization.J Nucl Agric Sci, 2012, 26: 17-21 (in Chinese with English abstract) | |
| [44] | Zhang X, Pan H, Bai G.Quantitative trait loci responsible for Fusarium head blight resistance in Chinese landrace Baishanyuehuang.Theor Appl Genet, 2012, 125: 495-502 |
| [45] | Li T, Bai G, Wu S, Gu S.Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu.Euphytica, 2012, 185: 93-102 |
| [46] | 何中虎, 兰彩霞, 陈新民, 邹裕春, 庄巧生, 夏先春. 小麦条锈病和白粉病成株期抗性研究进展与展望. 中国农业科学, 2011, 44: 2193-2215 |
| He Z H, Lan C X, Chen X M, Zou Y C, Zhuang Q S, Xia X C.Progress and perspective in research of adult-plant resistance in stripe rust and powdery mildew in wheat.Sci Agric Sin, 2011, 44: 2193-2215 (in Chinese with English abstract) |
| [1] | 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356. |
| [2] | 郭星宇, 刘朋召, 王瑞, 王小利, 李军. 旱地冬小麦产量、氮肥利用率及土壤氮素平衡对降水年型与施氮量的响应[J]. 作物学报, 2022, 48(5): 1262-1272. |
| [3] | 付美玉, 熊宏春, 周春云, 郭会君, 谢永盾, 赵林姝, 古佳玉, 赵世荣, 丁玉萍, 徐延浩, 刘录祥. 小麦矮秆突变体je0098的遗传分析与其矮秆基因定位[J]. 作物学报, 2022, 48(3): 580-589. |
| [4] | 冯健超, 许倍铭, 江薛丽, 胡海洲, 马英, 王晨阳, 王永华, 马冬云. 小麦籽粒不同层次酚类物质与抗氧化活性差异及氮肥调控效应[J]. 作物学报, 2022, 48(3): 704-715. |
| [5] | 刘运景, 郑飞娜, 张秀, 初金鹏, 于海涛, 代兴龙, 贺明荣. 宽幅播种对强筋小麦籽粒产量、品质和氮素吸收利用的影响[J]. 作物学报, 2022, 48(3): 716-725. |
| [6] | 马红勃, 刘东涛, 冯国华, 王静, 朱雪成, 张会云, 刘静, 刘立伟, 易媛. 黄淮麦区Fhb1基因的育种应用[J]. 作物学报, 2022, 48(3): 747-758. |
| [7] | 王洋洋, 贺利, 任德超, 段剑钊, 胡新, 刘万代, 郭天财, 王永华, 冯伟. 基于主成分-聚类分析的不同水分冬小麦晚霜冻害评价[J]. 作物学报, 2022, 48(2): 448-462. |
| [8] | 陈新宜, 宋宇航, 张孟寒, 李小艳, 李华, 汪月霞, 齐学礼. 干旱对不同品种小麦幼苗的生理生化胁迫以及外源5-氨基乙酰丙酸的缓解作用[J]. 作物学报, 2022, 48(2): 478-487. |
| [9] | 徐龙龙, 殷文, 胡发龙, 范虹, 樊志龙, 赵财, 于爱忠, 柴强. 水氮减量对地膜玉米免耕轮作小麦主要光合生理参数的影响[J]. 作物学报, 2022, 48(2): 437-447. |
| [10] | 马博闻, 李庆, 蔡剑, 周琴, 黄梅, 戴廷波, 王笑, 姜东. 花前渍水锻炼调控花后小麦耐渍性的生理机制研究[J]. 作物学报, 2022, 48(1): 151-164. |
| [11] | 孟颖, 邢蕾蕾, 曹晓红, 郭光艳, 柴建芳, 秘彩莉. 小麦Ta4CL1基因的克隆及其在促进转基因拟南芥生长和木质素沉积中的功能[J]. 作物学报, 2022, 48(1): 63-75. |
| [12] | 韦一昊, 于美琴, 张晓娇, 王露露, 张志勇, 马新明, 李会强, 王小纯. 小麦谷氨酰胺合成酶基因可变剪接分析[J]. 作物学报, 2022, 48(1): 40-47. |
| [13] | 李玲红, 张哲, 陈永明, 尤明山, 倪中福, 邢界文. 普通小麦颖壳蜡质缺失突变体glossy1的转录组分析[J]. 作物学报, 2022, 48(1): 48-62. |
| [14] | 罗江陶, 郑建敏, 蒲宗君, 范超兰, 刘登才, 郝明. 四倍体小麦与六倍体小麦杂种的染色体遗传特性[J]. 作物学报, 2021, 47(8): 1427-1436. |
| [15] | 王艳朋, 凌磊, 张文睿, 王丹, 郭长虹. 小麦B-box基因家族全基因组鉴定与表达分析[J]. 作物学报, 2021, 47(8): 1437-1449. |
|
||
