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作物学报 ›› 2018, Vol. 44 ›› Issue (04): 505-511.doi: 10.3724/SP.J.1006.2018.00505

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用Fhb1基因功能标记选择提高黄淮冬麦区小麦品种对赤霉病的抗性

张宏军1(), 宿振起2,3, 柏贵华3,4, 张旭5, 马鸿翔5, 李腾1, 邓云6, 买春艳7, 于立强8, 刘宏伟1, 杨丽1, 李洪杰1,*(), 周阳1,*()   

  1. 1 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程, 北京100081
    2 河北省农林科学院粮油作物研究所, 河北石家庄050035
    3 Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA
    4 USDA-ARS / Hard Winter Wheat Genetics Research Unit, Manhattan, KS 66506, USA
    5 江苏省农业科学院 / 江苏省农业生物学重点实验室, 江苏南京210014
    6 福建省南平市农业科学研究所, 福建建阳354200
    7 新乡矮败小麦育种技术创新中心, 河南新乡453731
    8 石家庄市农林科学研究院赵县试验基地, 河北赵县051530
  • 收稿日期:2017-07-19 接受日期:2018-01-08 出版日期:2018-01-29 网络出版日期:2018-01-29
  • 通讯作者: 李洪杰,周阳
  • 作者简介:

    zhanghongjun01@caas.cn

  • 基金资助:
    本研究由国家重点研发计划项目(2016YFD0101802, 2017YFD0100600)和作物分子育种国家工程实验室项目资助

Improvement of Resistance of Wheat Cultivars to Fusarium Head Blight in the Yellow-Huai Rivers Valley Winter Wheat Zone with Functional Marker Selection of Fhb1 Gene

Hong-Jun ZHANG1(), Zhen-Qi SU2,3, Gui-Hua BAI3,4, Xu ZHANG5, Hong-Xiang MA5, Teng LI1, Yun DENG6, Chun-Yan MAI7, Li-Qiang YU8, Hong-Wei LIU1, Li YANG1, Hong-Jie LI1,*(), Yang ZHOU1,*()   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2 Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei, China
    3 Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA
    4 USDA-ARS / Hard Winter Wheat Genetics Research Unit, Manhattan, KS 66506, USA
    5 Jiangsu Academy of Agricultural Sciences / Jiangsu Provincial Key Laboratory of Agrobiology, Nanjing 210014, Jiangsu, China
    6 Nanping Institute of Agricultural Sciences of Fujian Province, Jianyang 354200, Fujian, China
    7 Xinxiang Innovation Center for Breeding Technology of Dwarf-Male-Sterile Wheat, Xinxiang 453731, Henan, China
    8 Zhaoxian Experiment Station, Shijiazhuang Academy of Agriculture and Forestry Sciences, Zhaoxian 515300, Hebei, China
  • Received:2017-07-19 Accepted:2018-01-08 Published:2018-01-29 Published online:2018-01-29
  • Contact: Hong-Jie LI,Yang ZHOU
  • Supported by:
    This study was supported by the National Key Research and Development Program of China (2016YFD0101802, 2017YFD0100600) and the National Engineering Laboratory of Crop Molecular Breeding.

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摘要：

赤霉病已上升为黄淮冬麦区的主要病害, 提高小麦品种对赤霉病的抗性成为该麦区主要的育种目标之一。宁麦9号、生选6号、建阳798、建阳84、苏麦3号和宁麦13均携带Fhb1基因, 对赤霉病表现中抗水平以上。本研究以这6个品种(系)为供体, 分别与高感赤霉病的周麦16矮败小麦近等基因系杂交和回交, 构建6个回交群体。利用Fhb1基因的KASP标记在回交后代中进行基因型分析, 分别选择携带和不携带Fhb1基因的可育株, 对后代株系进行单花滴注接种鉴定和田间病圃自然鉴定。回交后代携带Fhb1家系整体抗性达到中感, 比不携带Fhb1家系的平均病小穗数低4.2 (P < 0.01), 平均病情指数低4.0, 比轮回亲本周麦16的平均病小穗数和病情指数分别低8.1 (P < 0.01)和28.4 (P < 0.01)。不同供体品种(系)回交后代在赤霉病抗性上表现出明显差异, 以生选6号为供体的回交后代家系抗性表现最好。本研究表明, 利用Fhb1基因分子标记辅助选择技术能够有效地提高黄淮冬麦区小麦品种的赤霉病抗性水平。

关键词: 小麦, 赤霉病抗性, Fhb1基因, 分子标记辅助选择

Abstract:

Fusarium head blight (FHB) becomes a major disease in the wheat production of the Yellow-Huai Rivers Valley Winter Wheat Zone of China. Improving FHB resistance is one of the major breeding targets in this region. The donors of Fhb1 gene, including Ningmai 9, Shengxuan 6, Jianyang 798, Jianyang 84, Sumai 3, and Ningmai 13, were moderately or highly resistant to FHB. Six backcrossing populations were developed by crossing these donors with a dwarfing and male-sterile wheat near isogenic line Zhoumai 16, and backcrossing with Zhoumai 16. The progenies were subjected to phenotypic evaluation of FHB by both the floret-inoculation method and natural infection in a field disease nursery. Fhb1 was detected by a functional Kompetitive Allele Specific PCR (KASP) marker. The backcrossing progenies with Fhb1 gene were moderately susceptible to FHB. Compared with the backcrossing progenies without Fhb1 gene, mean number of diseased spikelets and disease index reduced by 4.2 (P < 0.01) and 4.0 in the progenies with Fhb1 gene, respectively. The mean number of diseased spikelets and disease index for the backcrossing progenies with Fhb1 gene were 8.1 (P < 0.01) and 28.4 (P < 0.01) lower than those of the recurrent parent Zhoumai 16. There were significant differences in resistance among the backcrossing progenies from different donors. The progenies from Shengxuan 6 had better performance in number of diseased spikelets and disease index compared with those from any other donors. The results from this study indicate that Fhb1 gene can efficiently improve the FHB resistance of wheat cultivars grown in the Yellow-Huai Rivers Valley Winter Wheat Zone.

Key words: Triticum aestivum L., Fusarium head blight resistance, Fhb1 gene, molecular marker-assisted selection

图1

单花滴注接种鉴定携带和不携带供体Fhb1基因的回交后代、轮回亲本及对照赤霉病抗性表现携带宁麦9号(A)、生选6号(B)、建阳798 (C)、建阳84 (D)、苏麦3号(E)和宁麦13 (F) Fhb1基因回交后代的病小穗数明显少于不携带供体Fhb1基因的回交后代。轮回亲本周麦16较中感对照淮麦20 (G)、高感对照安农8455较中抗对照扬麦158 (H)具有更多的病小穗数。+: 携带Fhb1基因; –: 不携带Fhb1基因; RP: 轮回亲本; MS: 中感; HS: 高感; MR: 中抗。"

图2

携带不同供体Fhb1基因回交后代与轮回亲本、中感对照及不携带Fhb1基因回交后代间病小穗数和病情指数比较携带Fhb1基因的所有回交后代与轮回亲本周麦16 (A)、中感对照淮麦20 (B)和不携带Fhb1基因的所有回交后代(C)病小穗数和病情指数比较。携带宁麦9号Fhb1基因的回交后代(D)、携带生选6号Fhb1基因的回交后代(E)、携带建阳798 Fhb1基因的回交后代(F)、携带建阳84 Fhb1基因的回交后代(G)、携带苏麦3号Fhb1基因的回交后代(H)和携带宁麦13 Fhb1基因的回交后代(I)分别与其不携带Fhb1基因的回交后代间病小穗数和病情指数比较。NDS: 病小穗数(人工接种); DI: 病情指数(自然发病)。++: 携带Fhb1基因的所有回交后代; RP: 轮回亲本; MS: 中感对照; ––: 不携带Fhb1基因的所有回交后代; +: 携带Fhb1基因的回交后代; –: 不携带Fhb1基因的回交后代。误差线上*和**分别表示在P < 0.05和P < 0.01水平下差异显著。"

表1

携带不同供体Fhb1基因的回交后代间病小穗数和病情指数比较"

品种(系)或回交后代
Cultivars (lines) or backcrossing progenies		 病小穗数 No. of diseased spikelets 病情指数Disease index
平均值±标准差 Mean±SD 范围
Range		 平均值±标准差 Mean±SD 范围
Range
宁麦9号回交后代 Ningmai 9 backcrossing progenies 6.5±3.4 de 1.0-13.0 70.9±6.1 ab 61.8-78.8
生选6号回交后代 Shengxuan 6 backcrossing progenies 3.9±3.2 f 1.0-10.0 56.4±7.5 c 48.8-63.8
建阳798回交后代 Jianyang 798 backcrossing progenies 9.5±4.8 c 1.0-20.0 65.6±10.9 c 52.4-76.4
建阳84回交后代 Jianyang 84 backcrossing progenies 5.6±3.9 e 1.0-12.0 65.9±19.7 ab 34.4-86.1
苏麦3号回交后代 Sumai 3 backcrossing progenies 4.8±4.1 ef 1.0-13.0 70.8±12.3 ab 48.1-84.7
宁麦13回交后代 Ningmai 13 backcrossing progenies 7.4±5.6 d 1.0-20.0 81.5±16.4 ab 63.2-95.0
轮回亲本-周麦16 Recurrent parent-Zhoumai 16 14.4±6.0 b 7.0-20.0 96.1±1.4 a 95.0-97.6
中感对照-淮麦20 MS control-Huaimai 20 6.2±2.2 de 2.0-12.0 68.6±11.3 ab 56.9-81.0
中抗对照-扬麦158 MR control-Yangmai 158 3.2±2.2 f 1.0-10.0 — —
高感对照-安农8455 HS control-Annong 8455 19.7±1.4 a 12.0-20.0 — —
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