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作物学报 ›› 2019, Vol. 45 ›› Issue (11): 1764-1769.doi: 10.3724/SP.J.1006.2019.82066

• 研究简报 • 上一篇    

节水抗旱稻恢复系的抗褐飞虱分子标记辅助选育及抗性评价

张安宁1,2,刘毅1,2,王飞名1,谢岳文2,孔德艳1,聂元元3,张分云1,毕俊国1,余新桥1,刘国兰1,罗利军1,2,*()   

  1. 1 上海市农业生物基因中心, 上海 201106
    2 华中农业大学植物科学技术学院, 湖北武汉 430070
    3 江西省超级水稻研究发展中心, 江西南昌 330200
  • 收稿日期:2018-12-20 接受日期:2019-06-12 出版日期:2019-11-12 网络出版日期:2019-07-09
  • 通讯作者: 罗利军
  • 作者简介:E-mail: zan@sagc.org.cn
  • 基金资助:
    基金：本研究由上海市种业发展项目(2019-02-08-00-08-F01110);上海市科技兴农重点攻关(2018-02-08-00-08-F01553);国家重点研发计划(2017YFD0100304)

Pyramiding and evaluation of brown planthopper resistance genes in water-saving and drought-resistance restorer line

ZHANG An-Ning1,2,LIU Yi1,2,WANG Fei-Ming1,XIE Yue-Wen2,KONG De-Yan1,NIE Yuan-Yuan3,ZHANG Fen-Yun1,BI Jun-Guo1,YU Xin-Qiao1,LIU Guo-Lan1,LUO Li-Jun1,2,*()   

  1. 1 Shanghai Agrobiological Gene Center, Shanghai 201106, China
    2 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
    3 Jiangxi Super Rice Research and Development Center, Nanchang 330200, Jiangxi, China
  • Received:2018-12-20 Accepted:2019-06-12 Published:2019-11-12 Published online:2019-07-09
  • Contact: Li-Jun LUO
  • Supported by:
    This study was supported by the Shanghai Seed Industry Development Project(2019-02-08-00-08-F01110);the Shanghai Agriculture Applied Technology Development Program(2018-02-08-00-08-F01553);the National Key Research and Development Program of China(2017YFD0100304)

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摘要：

褐飞虱是水稻的主要害虫之一, 利用水稻抗褐飞虱基因培育抗虫品种是目前公认最经济有效、环境友好的策略。本研究利用水稻功能基因组已克隆的抗褐飞虱基因, 通过分子标记辅助选择和常规回交育种相结合的方法, 将抗褐飞虱基因Bph6、Bph9、Bph14和Bph15单独和聚合导入到节水抗旱稻恢复系旱恢3号, 获得了一系列含有单基因、双基因、三基因和四基因的改良系。采用标准苗期集团筛选法进行褐飞虱抗性鉴定, 评价这些基因在旱恢3号背景下的效应及相互作用。表明单基因改良系中, Bph9的抗性最强, 且Bph9 > Bph6 > Bph15 > Bph14; 在聚合改良系中, 抗性均优于单基因改良系, 四基因聚合改良系的抗性最强, 不同基因型组合的抗性效应是Bph6+Bph9+Bph14+Bph15 > Bph6+Bph9 > Bph6+Bph9+Bph14 > Bph6+Bph9+Bph15 > Bph6+Bph14+Bph15 > Bph9+Bph14+Bph15 > Bph14+Bph15。在自然条件下, 改良系与旱恢3号在株高、有效穗和千粒重等农艺性状上差异不显著, 其他性状与旱恢3号相仿或略差。本试验表明单独和聚合导入Bph6、Bph9、Bph14和Bph15基因能显著提高节水抗旱稻恢复系的褐飞虱抗性, 这4个基因的加性效应明显, 可为今后节水抗旱稻抗褐飞虱育种提供理论依据和材料基础。

关键词: 育种, 褐飞虱, 抗性基因, 分子标记辅助选择, 抗性评价, 节水抗旱稻

Abstract:

The brown planthopper (Nilaparvata lugens St?l, BPH) is the most serious pest threat to rice production across Asia. Increasing host-plant resistance is the most economical and ecological strategy for controlling this pest. The objective of this study was to survey the resistance effects of different R genes to brown planthopper, reveal its influence on agronomic traits, and provide insights into molecular breeding of rice with resistance to brown planthopper. In this research, the brown planthopper R genes Bph6, Bph9, Bph14, and Bph15 were introgressed into the water-saving and drought-resistant rice restorer line ‘Hanhui 3’ through marker-assisted backcrossing scheme. The standard seedling group screening method was used to identify the resistance of the improved lines. Among single-gene improved lines, the order of the R genes effects was Bph9 > Bph6 > Bph15 > Bph14, and among pyramiding improved lines, that was Bph6+Bph9+Bph14+Bph15 > Bph6+Bph9 > Bph6+Bph9+Bph14 > Bph6+Bph9+Bph15 > Bph6+Bph14+Bph15 > Bph9+Bph14+Bph15 > Bph14+Bph15. Furthermore, the survey of agronomic traits demonstrated that there were no significant differences between the 11 improved lines and recurrent parent ‘Hanhui 3’ in plant height, panicle number per plant and 1000-grain weight. These results suggest that the introgression of Bph6, Bph9, Bph14, and Bph15 genes by molecular marker-assisted selection technology could enhance the resistance to brown planthopper and improve breeding efficiency.

Key words: breeding, brown planthopper, resistance gene, marker-assisted selection, resistance evaluation, water-saving and drought-resistance rice

图1

抗褐飞虱恢复系的选育"

图2

抗褐飞虱基因的分子标记检测 A: 检测Bph6; B: 检测Bph9; C: 检测Bph14; D: 检测Bph15。M: D2000 marker; P1: 旱恢3号; P2: 供体; 1~11: 改良系18R1~18R11。"

表1

苗期抗褐飞虱鉴定结果"

编号
No.		 鉴定编号
Identification no.		 基因型
Genotype		 抗性表现
Resistance performance		 抗性级别
Resistance level#
18R1 18FS30 Bph6 2.60±0.81 抗虫 R
18R2 18FS72 Bph9 2.40±0.35 抗虫 R
18R3 18FS32 Bph14 5.20±0.76 中抗 MR
18R4 18FS71 Bph15 4.30±0.97 中抗 MR
18R5 18FS86 Bph6+Bph9 1.10±0.21 高抗 HR
18R6 18FS83 Bph14+Bph15 1.93±0.41 高抗 HR
18R7 18FS90 Bph6+Bph9+Bph14 1.27±0.27 高抗 HR
18R8 18FS88 Bph6+Bph9+Bph15 1.50±0.38 高抗 HR
18R9 18FS92 Bph6+Bph14+Bph15 1.60±0.31 高抗 HR
18R10 18FS94 Bph9+Bph14+Bph15 1.70±0.35 高抗 HR
18R11 18FS97 Bph6+Bph9+Bph14+Bph15 0.53±0.24 高抗 HR
旱恢3号 Hanhui 3 18FS26 8.93±0.07 高感 HS
TN1 8.80±0.12 高感 HS

表2

改良品系的主要农艺性状"

编号
No.		 基因型
Genotype		 株高
Plant height (cm)		 单株有效穗
Panicles
per plant		 穗长
Panicle length
(cm)		 每穗总粒数
Grains per
panicle		 结实率
Grain fertility
(%)		 千粒重
1000-Grain weight (g)		 单株产量
Grain yield
per plant (g)
18R1 Bph6 121.00±1.00 13.67±3.21 21.49±0.58 200.30±2.84** 83.35±3.04 26.35±0.88 32.34±0.70*
18R2 Bph9 119.68±1.64 9.88±0.55 25.53±0.87** 202.65±5.16** 77.62±1.78 24.79±1.66 33.94±1.74
18R3 Bph14 129.00±6.24 10.00±4.00 24.88±1.80 199.98±7.81** 90.37±1.14 25.00±1.20 29.04±0.73**
18R4 Bph15 120.48±1.36 10.70±1.00 26.13±0.45** 200.22±3.20** 79.16±2.39 24.32±0.54 34.94±1.47
18R5 Bph6+Bph9 120.65±2.67 10.58±0.64 25.75±0.57** 201.66±2.29** 76.68±0.98* 24.44±1.58 31.99±3.46*
18R6 Bph14+Bph15 119.71±2.21 9.88±0.64 26.08±0.49** 198.87±7.43** 80.37±1.66 26.07±1.82 30.64±1.71**
18R7 Bph6+Bph9+Bph14 118.12±1.46 10.50±1.04 24.90±0.48* 194.34±2.80** 71.64±1.30** 26.21±1.12 34.90±0.71
18R8 Bph6+Bph9+Bph15 124.19±0.13 10.04±0.69 25.13±0.85* 205.54±5.21** 71.86±1.73** 24.44±0.88 32.75±2.89
18R9 Bph6+Bph14+Bph15 119.89±1.59 10.95±0.23 24.94±0.61* 200.59±4.13** 68.69±1.13** 24.87±1.73 36.47±1.04
18R10 Bph9+Bph14+Bph15 118.41±3.68 10.99±0.53 26.14±0.62** 201.56±2.44** 69.90±3.57** 26.53±0.52 31.19±1.82*
18R11 Bph6+Bph9+Bph14+Bph15 121.12±2.63 11.33±0.56 25.82±0.36** 200.79±6.06** 57.26±2.73** 26.40±1.21 30.38±1.89**
Hanhui 3 117.00±4.90 10.00±0.82 22.80±0.41 240.69±21.35 86.24±6.49 25.84±1.62 42.11±6.78
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