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作物学报 ›› 2022, Vol. 48 ›› Issue (2): 380-395.doi: 10.3724/SP.J.1006.2022.04273

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

棉籽油分和3种主要脂肪酸含量QTL分析

张艳波(), 王袁(), 冯甘雨, 段慧蓉, 刘海英*()   

  1. 云南大学农学院, 云南昆明 650091
  • 收稿日期:2020-12-14 接受日期:2021-07-12 出版日期:2022-02-12 网络出版日期:2021-08-10
  • 通讯作者: 刘海英
  • 作者简介:张艳波, E-mail: zhangyanbo@mail.ynu.edu.cn;
    王袁, E-mail: wymorning@outlook.com
  • 基金资助:
    本研究由国家自然科学基金项目(31760404);云南大学农学学科建设项目资助(C176210302)

QTLs analysis of oil and three main fatty acid contents in cottonseeds

ZHANG Yan-Bo(), WANG Yuan(), FENG Gan-Yu, DUAN Hui-Rong, LIU Hai-Ying*()   

  1. School of Agriculture, Yunnan University, Kunming 650091, Yunnan, China
  • Received:2020-12-14 Accepted:2021-07-12 Published:2022-02-12 Published online:2021-08-10
  • Contact: LIU Hai-Ying
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31760404);the Agricultural Discipline Construction Project of Yunnan University(C176210302)

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摘要：

本研究对棉籽油分、棕榈酸、油酸和亚油酸含量进行了不同遗传体系的QTL分析, 为相关性状挖掘出更多有用的基因信息。分别于2017年和2018年, 利用陆地棉亲本HS46 (P1)和MARCABUCAG8US-1-88 (P2)所构建的188个重组近交系分别与双亲杂交构建F1群体BC (P1)和BC (P2)。基于这些回交群体种子, 采用专为种子性状设计的母体和胚核基因组QTL定位的混合线性遗传模型及QTL Network-CL-2.0-Seed软件, 对棉籽油分、棕榈酸、油酸和亚油酸含量进行QTL定位分析。共检测到7个控制棉籽油分含量、3个控制棕榈酸含量、2个控制油酸含量和3个控制亚油酸含量的QTL, 均具有显著或极显著的源自母体和胚2个核基因组的加性主效应, 其中有7个QTL的表型变异贡献率大于10%。研究结果可为这些性状的分子标记辅助选择育种提供更为可靠的参考, 为这些性状的分子遗传机制研究提供理论基础。

关键词: 棉花, QTL定位, 油分含量, 脂肪酸含量, 胚核基因组, 母体核基因组

Abstract:

In this study, in order to uncover more useful gene information for these traits, the analysis of QTLs controlling the contents of oil, palmitic acid, oleic acid, and linoleic acid in cottonseeds was conducted in different genetic systems. Based on a set of 188 recombinant inbred lines (RILs) derived from an intra-specific cross between two upland germplasms HS46 (P1) and MARCABUCAG8US-1-88 (P2), double backcross populations BC (P1) and BC (P2) were constructed in 2017 and 2018, respectively. BC (P1) and BC (P2) populations were obtained through backcrossing each of the two parents (HS46 and MARCABUCAG8US-1-88) with the 188 RILs, respectively. The cottonseeds from these backcross populations were used for QTLs analysis of oil, palmitic acid, oleic acid, and linoleic acid contents. To map QTLs for these traits, the mixed linear genetic model and the QTL Network-CL-2.0-Seed software, which were developed especially for seed traits, were applied. In total, seven QTLs for the oil content, three for the palmitic acid content, two for the oleic acid content, and three for the linoleic acid content were detected. Significant additive effects of QTLs from maternal plants and embryos were detected for all the QTLs. Seven QTLs, each of which explained more than 10% of total phenotypic variation, were found. The detected QTLs in the present study may give us better understanding of the molecular inheritance for these four traits, and provide more reliable information for molecular marker assisted breeding.

Key words: cotton, quantitative trait loci (QTL), oil content, fatty acid, embryo nuclear genome, maternal nuclear genome

表1

亲本和回交群体的棉籽油分及3种脂肪酸含量表现"

年份Year 性状
Trait		 回交群体BC(P1) 回交群体BC(P2) 亲本Parents
平均值
Mean		 标准差
SD		 最小值
Min.		 最大值
Max.		 偏度
Skew		 峰度
Kurt		 平均值
Mean		 标准差
SD		 最小值
Min.		 最大值
Max.		 偏度
Skew		 峰度
Kurt		 HS46 MAR
2017 油分含量
Oil content		 32.28 1.85 25.26 38.06 -0.23 0.12 33.05 1.98 27.59 37.30 -0.18 -0.17 31.01 a 33.49 b
棕榈酸含量
Palmitic acid content		 25.66 0.63 24.19 27.40 0.13 -0.07 25.73 0.67 23.82 27.75 -0.38 0.56 25.49 a 25.94 b
油酸含量
Oleic acid content		 17.86 0.80 15.84 20.53 0.34 0.34 17.51 1.01 14.88 20.34 -0.11 0.54 17.31 17.01
亚油酸含量
Linoleic acid content		 56.90 1.16 53.81 59.79 -0.13 -0.31 56.85 1.29 52.80 60.85 -0.14 0.88 57.55 a 56.81 b
2018 油分含量
Oil content		 32.86 0.92 29.58 35.08 -0.80 0.75 33.58 0.97 30.94 36.82 -0.22 0.29 29.70 A 32.69 B
棕榈酸含量
Palmitic acid content		 24.72 0.57 23.13 26.45 0.17 0.36 25.16 0.51 23.26 27.17 -0.37 0.09 24.63 A 25.22 B
油酸含量
Oleic acid content		 17.38 0.68 15.63 19.15 0.10 -0.07 17.02 0.59 15.24 18.85 -0.01 0.33 16.60 a 16.21 b
亚油酸含量
Linoleic acid content		 58.47 0.97 55.76 61.14 -0.05 0.21 58.06 0.70 56.16 59.80 -0.03 0.12 59.06 58.45

图1

棉籽油分、棕榈酸、油酸和亚油酸含量分别在BC (P1)和BC (P2)群体中分布(2017年和2018年)"

表2

棉籽4个品质性状的相关性分析"

性状
Trait		 油分
Oil content		 棕榈酸
Palmitic acid content		 油酸
Oleic acid content		 亚油酸
Linoleic acid content
油分Oil content 1.00000
棕榈酸Palmitic acid content 0.45145** 1.00000
油酸Oleic acid content -0.14629** 0.15922** 1.00000
亚油酸Linoleic acid content -0.13874** -0.64037** -0.73753** 1.00000

表3

棉籽油分及3种脂肪酸的QTL定位及其遗传效应"

性状
Trait		 QTL 连锁群
Chr. / LG		 标记区间
Marker interval		 位置Position
(cM)		 范围
Range
(cM)		 ae de am aeE1 aeE2 deE1 deE2 amE1 amE2
油分含量
Oil content		 qOil-3-1 Chr. 3 MUCS127(c3)-NAU808 4.8 2.0-7.3 9.49** 0.05 -9.32** -0.02 -0.05 0.11 -0.10 0.02 0.06
qOil-18-1 Chr. 18 JESPR204(c18)-CIR099(18) 0.0 0.0-1.0 -21.10** -0.10 20.88** 0.09 0.00 -0.00 0.00 -0.09 -0.00
qOil-18-2 Chr. 18 TM0409a-NAU1001 14.8 13.9-16.8 -5.02** -0.23** 5.24** -0.07 -0.04 -0.07 0.07 0.07 0.03
qOil-18-3 Chr. 18 NAU5152b-NAU3053a 76.7 72.3-79.7 -0.32** 0.11* 0.50** -0.12* -0.01 0.08 -0.08 0.12* 0.01
qOil-LG3-1 lg3 NAU5146c-RAPD-I10-620 51.4 48.6-58.4 13.45** -0.01 -13.72** 0.07 0.08 0.00 -0.00 -0.07 -0.08
qOil-LG7-1 lg7 NAU983-TMB1791 0.0 0.0-2.0 0.81** -0.28** -0.90** -0.00 0.22** -0.22* 0.21** -0.00 -0.22**
qOil-LG8-1 lg8 NAU4919b*-NAU4960d 31.7 30.7-34.5 1.84** 0.05 -1.48** -0.16* 0.00 0.07 -0.09 0.16* -0.01
棕榈酸含量
Palmitic acid content		 qC16-0-15-1 Chr. 15 BNL1579-BNL4082(c15) 25.1 23.1-27.0 5.72** -0.06* -5.66** -0.02 -0.00 0.00 -0.00 0.02 0.00
qC16-0-LG3-1 lg3 NAU5146c-RAPD-I10-620 56.4 50.6-62.4 -3.88** 0.04 3.74** 0.11** -0.13** -0.00 0.00 -0.11** 0.13**
qC16-0-LG6-1 lg6 NAU4852-NAU1247 40.5 39.2-42.5 -2.02** -0.04* 2.10** -0.01 -0.00 0.05 -0.05 0.02 0.00
油酸含量
Oleic acid content		 qC18-1-18-1 Chr. 18 NAU5152a-NAU1232 44.6 40.6-47.6 -1.44** 0.01 1.28** 0.21** -0.20** 0.00 -0.00 -0.21** 0.20**
qC18-1-LG6-1 lg6 NAU456-NAU5328-170 19.9 17.9-21.9 1.74** -0.09** -1.83** 0.10** -0.02 -0.10* 0.09* -0.10** 0.02
亚油酸含量
Linoleic acid content		 qC18-2-6-1 Chr. 6 NAU932*-NAU2566 81.4 75.4-91.0 10.47** 0.01 -10.60** 0.04 0.00 0.11* -0.11* -0.04 -0.00
qC18-2-15-1 Chr. 15 BNL1579-BNL4082(c15) 25.1 23.1-27.0 -4.83** 0.08* 4.71** -0.27** 0.29** -0.00 0.00 0.26** -0.28**
qC18-2-LG6-1 lg6 NAU5328-170-RAPD-G17-120 23.0 22.0-24.0 -5.45** -0.09* 5.64** -0.00 -0.05 0.15** -0.15** 0.00 0.04

图2

棉籽油分及3种脂肪酸含量QTL在胚和母体植株核基因组上的位置"

表4

棉籽油分及3种脂肪酸的QTL遗传贡献率"

性状Trait QTL R2 (am) R2 (ae) R2 (de) R2 (amE) R2 (aeE) R2 (deE) R2 (T)
油分含量
Oil content		 qOil-3-1 1.1 1.4 0.0 0.0 0.0 0.0 2.5
qOil-18-1 5.3 6.7 0.0 0.0 0.0 0.0 12.0
qOil-18-2 0.3 0.4 0.0 0.0 0.0 0.0 0.7
qOil-18-3 0.0 0.0 0.0 5.4 0.0 0.0 5.4
qOil-LG3-1 2.3 2.7 0.0 0.0 0.0 0.0 5.0
qOil-LG7-1 0.0 0.0 0.0 0.0 21.3 6.2 27.5
qOil-LG8-1 0.0 0.1 0.0 8.6 0.0 0.0 8.7
棕榈酸含量
Palmitic acid content		 qC16-0-15-1 5.0 6.4 0.0 0.0 0.0 0.0 11.4
qC16-0-LG3-1 2.2 3.0 0.0 11.6 19.6 0.0 36.5
qC16-0-LG6-1 0.7 0.8 0.0 0.0 0.0 0.0 1.5
油酸含量
Oleic acid content		 qC18-1-18-1 1.4 2.5 0.0 8.0 8.8 0.0 20.9
qC18-1-LG6-1 3.3 3.7 0.0 1.8 0.0 0.6 9.4
亚油酸含量
Linoleic acid content		 qC18-2-6-1 5.5 6.7 0.0 0.0 0.0 0.9 13.1
qC18-2-15-1 1.1 1.4 0.0 14.2 20.6 0.0 37.3
qC18-2-LG6-1 1.6 1.8 0.0 0.0 0.0 1.7 5.1

附表1

棉籽4个性状QTL位点相关分子标记信息"

染色体/连锁群
Chr./Linkage group		 分子标记
Molecular markers		 引物
Primers
(5°-3°)		 标记类型
Marker type		 标记引物来源
Source of marker primer		 标记来源
Source of marker		 重复基序
Repeat motif
Chr3 MUCS127(c3) F: TTTGTACTCATGTTCCCGCC;
R: GCATGCTTGAAGAAGGTTCC		 SSR Park et al.[40] G. arboreum EST TCTT(2)CTT(4)
Chr3 NAU808 F: GATGGCTACCTCCCTTTGTA;
R: CGTAAGGAAGCCTAGCAAAA		 SSR Han et al. [41,42] G. hirsutum EST none
Chr18 JESPR204(c18) F: CTCCAGGTTCAATGGTCTG;
R: GCCATGTTGGACAAGTAGTC		 SSR Reddy et al.[43] G. hirsutum genome (CTT)20
Chr18 CIR099(18) F: ATGATTCAAGTCGCGT;
R: TTCAAGGCTGAGTCAAA		 SSR Nguyen et al.[44] G. hirsutum genome (GT)8
Chr18 TMO409a F: CAGAGGACGAAGGTAGCAG;
R: TGGTGGGTTTCACTTTCACA		 SSR USDA-ARS G. hirsutum genome (CA)10+(GA)5
Chr18 NAU1001 F: TAAAGGAAGAAAACCCACCA;
R: TTTCTCTTCAACAGCAGCAG		 SSR Han et al.[41,42] G. hirsutum EST (ACA )7
Chr18 NAU5152b F: TCCTTTCTACCCATGCCTAC;
R: GTCACGAGAAGCAGAGGACT		 SSR Han et al.[41,42] G. hirsutum EST (tct)9
Chr18 NAU3053a F: TTGGAGGTGTCAGATCCTCT;
R: AAGATCCATGACCCAGAAAA		 SSR Han et al.[41,42] G. hirsutum EST (at)13
LG3 NAU5146c F: ATCTCCATTCCCTCTCCTCT;
R: ATAAACGCAACGGATAAAGG		 SSR Han et al.[41,42] G. hirsutum EST (ttta)5
LG3 RAPD-I10-620 GTCCACACGG RAPD 购于上海赛百盛生物技术公司
(The marker primer purchased from SBS Genetech Co.,Ltd)		 G. hirsutum genome None
LG7 NAU983 F: GAAGGTCATGTTTTGCATTG;
R: AGCCATCAAATGACCAGTCT		 SSR Han et al.[41,42] G. hirsutum EST (CAGCAT)3
LG7 TMB1791 F: GAGATCGTTTATCATTCCCACA;
R: TCTACCGGCCTCGTAAGCTA		 SSR Yu et al.[45] G. hirsutum genome (GA)15+(CA)8
LG8 NAU4919b* F: ATCTAATGGGGGAGGAAGAG;
R: CTCCTTGAAACCCTTTGAGA		 SSR Han et al.[41,42] G. hirsutum EST (cca)6
LG8 NAU4960d F: CGGCCTACCTCTTCCTCT;
R: TTAACGACAATTCCATCGTG		 SSR Han et al.[41,42] G. hirsutum EST (tcc)6
Chr15 BNL4082(c15) F:GTAAAATGAAATAAAATAAAAGGAGAGA;
R: TTCAACACCGCCAAACATAA		 SSR Brookhaven National Laboratory, NY, USA G. hirsutum genome (GA)24
LG3 NAU4852 F: ATCTCAAGGCCCAAATTACA;
R: CCTAAATTGTCAAGCGAGGT		 SSR Han et al.[41,42] G. hirsutum EST (cat)6
LG3 NAU1247 F: AATTGGCTTCCAAGAGACTG;
R: CACCATCATCATCAAACTCG		 SSR Han et al.[41,42] G. hirsutum EST (AAG)8
Chr18 NAU5152a F: TCCTTTCTACCCATGCCTAC;
R: GTCACGAGAAGCAGAGGACT		 SSR Han et al.[41,42] G. hirsutum EST (tct)9
Chr18 NAU1232 F：GTGGAATGTGGTGATGAGAA;
R：CAATCCCACCCCATATCTA		 SSR Han et al.[41,42] G. hirsutum EST (TGTA)5
LG6 NAU456 F: GAAGGAGGGCGAAAAACATA;
R: TTGGAGATTACGCGACCTTC		 SSR Han et al.[41,42] G. hirsutum EST (CAC )8
LG6 NAU5328-170 F: AATGAGAGTGGCGGCTATAC;
R: CTTATAGCTCTGGGCAGCAT		 SSR Han et al.[41,42] G. hirsutum EST (aatgca)3
Chr6 NAU932* F: ACAAAGCAAATATGGGTTCC;
R: CCCCCTTAAAATGGAAGAAG		 SSR Han et al.[41,42] G. hirsutum EST (GGTGAA)3
Chr6 NAU2566 F: CAAAATCCACCCCCTCCT;
R: CCCTTCCCAGGAAAGTTTAT		 SSR Han et al.[41,42] G. hirsutum EST (aata)4
Chr15 BNL1579 F: CATGAGAACTTCATGCATATTCA;
R: ATTTAAGTGCCTCCGTCCCT		 SSR Brookhaven National Laboratory, NY, USA G. hirsutum genome (AG)17
LG6 RAPD-G17-120 GACGGATCAG RAPD 购于上海赛百盛生物技术公司
(The marker primer purchased from SBS Genetech Co., Ltd)		 G. hirsutum genome None
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