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作物学报 ›› 2022, Vol. 48 ›› Issue (2): 511-517.doi: 10.3724/SP.J.1006.2022.11025

• 研究简报 • 上一篇    下一篇

小麦-中间偃麦草2A/6St代换系014-459的分子细胞遗传学鉴定

陶军1,2(), 兰秀锦1,*()   

  1. 1四川农业大学小麦研究所, 四川成都 611130
    2绵阳市农业科学研究院, 四川绵阳 621023
  • 收稿日期:2021-03-09 接受日期:2021-06-16 出版日期:2022-02-12 网络出版日期:2021-06-29
  • 通讯作者: 兰秀锦
  • 作者简介:E-mail: tj4bmy@163.com
  • 基金资助:
    本研究由国家自然科学基金项目资助(31970243);This study was supported by the National Natural Science Foundation of China(31970243)

Molecular cytogenetic identification of wheat-Thinopyrum intermedium 2A/6St substitution strain 014-459

TAO Jun1,2(), LAN Xiu-Jin1,*()   

  1. 1Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
    2Mianyang Academy of Agricultural Sciences, Mianyang 621023, Sichuan, China
  • Received:2021-03-09 Accepted:2021-06-16 Published:2022-02-12 Published online:2021-06-29
  • Contact: LAN Xiu-Jin

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摘要：

中间偃麦草是小麦遗传改良的有用资源, 育成了大量的小麦-中间偃麦草附加系、代换系及部分双二倍体。中4是小麦-中间偃麦草部分双二倍体, 很方便与普通小麦杂交并被广泛用于小麦的遗传改良。014-459是中4与普通小麦杂交后代, 具有一些特殊特性, 材料014-459与一些普通小麦杂交, 无论正反交, 其F1表现为不育, 而与另一些普通小麦的杂交F1表现为可育, 此外, 材料014-459粗蛋白和湿面筋含量很高。基于014-459的这些特性, 猜测其可能具有中间偃麦草染色体片段, 为此对014-459进行了细胞学鉴定。FISH和GISH以及PLUG标记分析用来分析材料014-459的染色体组成情况。连续的FISH和GISH试验证实小麦-中间偃麦草部分双二部体中4与小麦杂交后代品系014-459的1对小麦2A染色体被来自中间偃麦草的1对St染色体代换, PLUG标记分析证实这1对St染色体属于第6同源群, 可能来自中间偃麦草的St染色体被代换进小麦中造成了材料014-459的一些特性。对品系014-459的分子细胞遗传学鉴定对促进中间偃麦草6St染色体在小麦中利用及小麦品质改良有积极作用。

关键词: GISH, FISH, 代换系, 拟鹅冠草, 细胞遗传学

Abstract:

Thinopyrum intermedium is a valuable resource for wheat genetic improvement, and numerous wheat-Th. intermedium chromosome addition and substitution lines and partial amphiploids have been developed. Zhong 4 is a wheat-Th. intermedium partial amphidiploid, which can easily hybridize with wheat and is extensively utilized for wheat improvement. The strain 014-459 was a progeny of partial amphidiploid Zhong 4 descendant of wheat and Th. intermedium with some special characteristics such as high crude protein and wet gluten content and partial F1 of common wheat cultivars and strain 014-459 were sterility while a few others were fertility despite reciprocal cross. Molecular cytogenetic identification of strain 014-459 were detected for speculated contained fragment of Th. intermedium chromosomes based on its special characters. Genomic composition of strain 014-459 was detected by FISH, GISH, and PLUG (polymerase chain reaction-based landmark unique gene) marker analysis. Combined FISH/GISH analysis confirmed that a pair of wheat 2A chromosomes in strain 014-459 of the hybrid progeny between wheat-Thinopyrum intermedium partial amphidiploid Zhong 4 and wheat was replaced by a pair of St chromosomes from Thinopyrum intermedium. PLUG marker analysis verified that this pair belonged to the sixth homologous group. The special characters of strain 014-459 might be attributable to the substitution of Th. intermedium St chromosome into wheat. The study of molecular cytogenetic identification of strain 014-459 was probably beneficial to quality improvement and utilization of 6St chromosome of Th. intermedium in wheat breeding.

Key words: GISH, FISH, substitution strain, Pseudoroegneria strigosa, cytogenetics

图1

减数分裂图 A、B为减数分裂中期I; C、D、E为后期I; F、G、H为末期I。"

图2

原位杂交及花粉母细胞减数分裂图 A: 品系014-459FISH图, Oligo-pSc119.2-2(绿), oligo-pTa535-2(红); B: 品系014-459GISH图, 中间偃麦草DNA作探针, 中国春DNA为封阻; C: 品系014-459GISH图, 拟鹅冠草DNA为探针, 中国春DNA为封阻; D: 品系014-459FISH图, St2-80为探针, 中国春DNA为封阻; E: 品系014-459花粉母细胞减数分裂中期GISH图, 探针为中间偃麦草DNA, 封阻为中国春DNA。"

图3

原位杂交图 A: 品系014-459FISH图, Oligo-pSc119.2-2(绿), oligo-pTa535-2(红); B: 品系014-459GISH图, 中间偃麦草DNA作探针, 中国春DNA为封阻; C: 品系014-459GISH图, 黑麦DNA为探针, 中国春DNA为封阻。"

图4

PLUG标记分析结果 A: TNAC1763扩增产物(Taq I酶切), 箭头示St特异带; B: TNAC1178扩增产物(Taq I酶切), 箭头示CS (中国春)特异带, 014-459及St (拟鹅冠草)未扩出这条带。"

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