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作物学报 ›› 2023, Vol. 49 ›› Issue (1): 153-166.doi: 10.3724/SP.J.1006.2023.14207

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

冷胁迫下甘蓝型冬油菜表达蛋白及BnGSTs基因家族的鉴定与分析

马骊1(), 白静2, 赵玉红3, 孙柏林3, 侯献飞4, 方彦1, 王旺田1, 蒲媛媛1, 刘丽君1, 徐佳1, 陶肖蕾1, 孙万仓1,*(), 武军艳1,*()   

  1. 1省部共建干旱生境作物学国家重点实验室 / 甘肃省油菜工程技术研究中心 / 甘肃农业大学农学院, 甘肃兰州 730070
    2张掖市农业科学院, 甘肃张掖 734000
    3甘肃亚盛农业研究院有限公司, 甘肃兰州 730030
    4新疆农业科学院经济作物研究所, 新疆乌鲁木齐 830091
  • 收稿日期:2021-11-04 接受日期:2022-05-05 出版日期:2023-01-12 网络出版日期:2022-05-19
  • 通讯作者: 孙万仓,武军艳
  • 作者简介:E-mail: mal@gsau.edu.cn
  • 基金资助:
    甘肃省青年科技计划项目(21JR7RA835);省部共建干旱生境作物学国家重点实验室(甘肃农业大学)开放基金课题(GSCS-2020-06);财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-12)

Protein and physiological differences under cold stress, and identification and analysis of BnGSTs in Brassica napus L.

MA Li1(), BAI Jing2, ZHAO Yu-Hong3, SUN Bo-Lin3, HOU Xian-Fei4, FANG Yan1, WANG Wang-Tian1, PU Yuan-Yuan1, LIU Li-Jun1, XU Jia1, TAO Xiao-Lei1, SUN Wan-Cang1,*(), WU Jun-Yan1,*()   

  1. 1State Key Laboratory of Aridland Crop Science / Gansu Research Center of Rapeseed Engineering and Technology / College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu, China
    2Zhangye Academy of Agricultural Sciences, Zhangye 734000, Gansu, China
    3Gansu Yasheng Agricultural Research Institute Co. Ltd, Lanzhou 730030, Gansu, China
    4Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, China
  • Received:2021-11-04 Accepted:2022-05-05 Published:2023-01-12 Published online:2022-05-19
  • Contact: SUN Wan-Cang,WU Jun-Yan
  • Supported by:
    Gansu Provincial Youth Science & Technology Department(21JR7RA835);Research Program Sponsored by State Key Laboratory of Aridland Crop Science (Gansu Agricultural University)(GSCS-2020-06);China Agriculture Research System of MOF and MARA(CARS-12)

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摘要：

谷胱甘肽S-转移酶(glutathione S-transferases, GST)参与调节植物生长、发育和逆境胁迫反应的许多方面。本研究利用双向电泳和质谱技术分析‘16VHNTS309’在冷胁迫下差异表达的蛋白质, 基于GO和KEGG分析鉴定出BE、APX、SOD、GST等参与冷胁迫反应的蛋白质。利用qRT-PCR和生理指标鉴定到响应冷胁迫的关键蛋白质GST, 采用同源克隆法克隆到‘16VHNTS309’的GST基因。该基因CDS长度为642 bp, 编码213个氨基酸, 是一个不稳定蛋白, 属于GST_N_3谷胱甘肽S-转移酶家族, 与甘蓝型油菜‘ZS11’序列相似性为99.22%, 与‘ZS11’和‘Vision’两者的氨基酸序列相比较发现第127位亮氨酸(L)突变为脯氨酸(P)。基因家族分析表明, 在甘蓝型油菜中共鉴定到153个BnGSTs成员, 按其功能主要分为Zeta、Phi、Theta、CHQ、DHAR、Lambda和Tau这七大类型, 大部分的BnGSTs属于Phi和Tau这2种类型。系统进化将BnGSTs分为12个亚家族, 亚家族I和VIII包含了较多的成员, BnGSTs不均匀的分布在18条染色体上, C06染色体上分布BnGSTs基因数量最多, 含有10个保守的蛋白质基序。BnGSTs基因家族中有99对基因存在共线性关系, 131个基因来自基因复制事件, 片段重复事件在BnGSTs基因的进化中起着重要作用。冷胁迫下BnaA02g35760D、BnaC06g20450D、BnaC06g35490D、BnaA02g03230D和BnaA02g35980D在强抗寒品种中的显著高表达, 是弱抗寒品种的7~12倍, 并且强抗寒品种具有较高的生理酶活性。另外, 在冷冻胁迫下鉴定到一些瞬时和持续表达的关键候选基因。这些结果为进一步研究BnGSTs基因在强抗寒甘蓝型冬油菜抗寒分子调控中的作用奠定基础。

关键词: 甘蓝型冬油菜, 冷胁迫, 蛋白质谱, GST基因家族, 表达模式

Abstract:

Glutathione S-transferases (GST) are involved in regulating many aspects of plant growth, development, and response to adversity stress. In this study, the differentially expressed proteins of ‘16VHNTS309’ under cold stress were analyzed using bidirectional electrophoresis and mass spectrometry, and proteins involved in the cold stress response, such as BE, APX, SOD, and GST, were identified based on GO and KEGG analyses. The key protein GST was identified using qRT-PCR and physiological indicators in response to cold stress, and the GST gene of ‘16VHNTS309’ was cloned using homologous cloning. This gene CDS length was 642 bp, and encoded 213 amino acids, which was an unstable protein and belonged to the GST_N_3 glutathione S-transferase family. The sequence similarity with Brassica napus ‘ZS11’ was 99.22%, and a comparison of the amino acid sequences of both ‘ZS11’ and ‘Vision’ revealed a mutation from leucine (L) to proline (P) at position 127. The gene family analysis showed that 153 BnGSTs members were identified in Brassica napus and were classified into seven main types according to their functions: Zeta, Phi, Theta, CHQ, DHAR, Lambda, and Tau. Most BnGSTs belonged to Phi and Tau types. Phylogeny divided BnGSTs into 12 subfamilies, subfamilies I and VIII contained more members. BnGSTs were unevenly distributed on 18 chromosomes, and the number of BNGSTs genes on C06 chromosome was the largest, it contained 10 conserved protein motifs. There were 99 pairs of genes in the BnGSTs gene family that were colinearly related, 131 genes from gene duplication events, and segmental duplication events played an important role in the evolution of BnGSTs genes. Significantly higher expression of BnaA02g35760D, BnaC06g20450D, BnaC06g35490D, BnaA02g03230D, and BnaA02g35980D in strong cold-resistant varieties was 7-12 times higher than that in weak cold-resistant varieties under cold stress. And the strong cold-resistant varieties had higher physiological enzyme activities. In addition, a number of key candidate genes were identified for transient and sustained expression under freezing stress. This result lays the foundation for further studies on the molecular regulation of BnGSTs genes for cold resistance in strong cold resistant for Brassica napus.

Key words: winter Brassica napus L., cold stress, protein mass spectrometry, GST gene family, expression pattern

表1

qRT-PCR引物序列"

基因名称
Gene name		 引物序列
Primer sequence (5'-3')		 产物大小
Product size (bp)
BE F: GATGGAACCCAGAACGAG; R: GGAATGCCACTGACAACG 110
SPI F: CCCCTCTTTATCGGACAG; R: GGAACGAACCCAACTCTA 89
APX F: GAGCGGTGAGAAGGAAGG; R: CTCGTCAGCAGCGTATTT 100
SOD F: GCCACATTTCAACCCTAACA; R: ACCACAAAGGCTCTTCCAAC 174
GST F: ATCAACATCTCCGACAAACC; R: CGTCAGAATCAGACACCCAC 100
RBP F: CGTGTTTATGTCGGCAATC; R: GCCACTGTCCCTGTCGTAG 114
β-Actin F: TGGGTTTGCTGGTGACGAT; R: TGCCTAGGACGACCAACAATACT 63

图1

冷胁迫下差异表达蛋白的鉴定 A: CK; B: 4℃处理24 h。箭头表示25个蛋白点被鉴定(P ≤ 0.05)。"

附图1

‘16VHNTS309’中蛋白点的放大图"

附表1

甘蓝型冬油菜响应冷胁迫的差异蛋白质鉴定"

蛋白点编号
Spot number		 基因登录号
Accession number		 蛋白质名称
Protein name		 分子质量
Molecular weight (kD)		 等电点
pI		 亚细胞定位
Cellular location		 差异倍数
Fold change
2 XP_013688985.1 RNA-binding protein CP29B 31,076 4.82 叶绿体Chloroplast NDT
3 XP_013656707.1 glycine-rich RNA-binding protein 10-like 16,038 5.24 微体Microbody 1.87±0.06
4 XP_013656707.1 glycine-rich RNA-binding protein 10-like 16,038 5.24 微体Microbody 0.49±0.01
6 XP_013687602.1 bifunctional enolase 2/transcriptional activator 47,556 5.56 内质网
Endoplasmic reticulum		 1.99±0.09
8 XP_013713332.1 oxygen-evolving enhancer protein 1-1 35,164 5.55 叶绿体Chloroplast 2.54±0.03
9 XP_013688985.1 RNA-binding protein CP29B 31,076 4.82 叶绿体Chloroplast NDT
11 XP_013649537.1 cysteine protease inhibitor WSCP 23,891 8.66 细胞外Extracellular 1.9±0.04
12 XP_013673398.1 ATP synthase subunit beta-2 53,800 5.39 细胞质Cytoplasm 2.52±0.06
13 XP_013659948.1 cysteine protease inhibitor WSCP-like 24,956 6.52 细胞外Extracellular NDC
14 XP_013678556.1 actin-7 41,665 5.31 细胞质Cytoplasm 2.01±0.11
15 XP_013641139.1 L-ascorbate peroxidase 1, cytosolic-like isoform X3 27,594 5.58 微体Microbody 2.63±0.06
16 XP_013697709.1 probable phospholipid hydroperoxide glutathione peroxidase 6 25,260 9.2 线粒体Mitochondrial 2.08±0.18
17 AIF75338.1 chloroplast ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit 19,852 7.59 细胞外Extracellular 2.21±0.05
18 XP_013720130.1 PREDICTED: superoxide dismutase [Cu-Zn] 2 21,343 6.79 细胞外Extracellular 2.75±0.11
19 XP_013649537.1 cysteine protease inhibitor WSCP 23,891 8.66 细胞外Extracellular 3.01±0.05
20 XP_013649391.1 nucleoside diphosphate kinase 1 16,384 6.29 微体Microbody 1.94±0.04
21 XP_013712048.1 CBS domain-containing protein CBSX3 22,650 8.71 线粒体Mitochondrial NDC
23 AIF75338.1 chloroplast ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit 19,852 7.59 细胞外Extracellular 3.33±0.39
24 XP_013649614.1 kunitz trypsin inhibitor 1-like 23,553 6.6 细胞外Extracellular NDC
25 XP_013641139.1 L-ascorbate peroxidase 1, cytosolic-like isoform X3 27,594 5.58 微体Microbody 3.41±0.22
26 AIF75338.1 chloroplast ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit 19,852 7.59 细胞外Extracellular 3.11±0.29
27 XP_013641880.1 glutathione S-transferase DHAR1, mitochondrial 23,533 5.76 细胞质Cytoplasm 2.1±0.1
28 XP_013666490.1 malate dehydrogenase 1, cytoplasmic-like 35,695 6.11 细胞外Extracellular 3.12±0.06
29 XP_013666490.1 malate dehydrogenase 1, cytoplasmic-like 35,695 6.11 细胞外Extracellular 2.91±0.21
30 XP_013649614.1 kunitz trypsin inhibitor 1-like 23,553 6.6 细胞外Extracellular NDC

图2

冷胁迫下‘16VHNTS309’差异蛋白的功能及通路注释 A: 细胞组分; B: 分子功能; C: 生物学过程; D: KEGG注释。"

图3

冷胁迫下差异蛋白质和基因表达模式及生理指标的分析 A: 蛋白质的表达谱与聚类分析; B: 6个具有急剧变化的DEPs采用qRT-PCR揭示其基因表达水平; C: 冷胁迫下的生理变化模式。小写字母表示所有品种和处理间的显著性(P ≤ 0.05)。"

图4

‘16VHNTS309’ GST基因序列比对 黑色: 3个品种氨基酸序列一致; 红色: 2个品种氨基酸序列一致; 白色: 氨基酸序列与另2个品种不一致。"

附图2

GST蛋白基本理化性质 A：跨膜螺旋结构；B：疏水性；C：信号肽；D：磷酸化位点；E：结构域；F：三级结构；G：氨基酸序列。"

图5

甘蓝型油菜GST基因的染色体分布图"

图6

甘蓝型油菜和拟南芥GST蛋白的系统发育树 亚家族I~XVII用不同的颜色标记。绿色三角形代表拟南芥, 黄色五角星代表甘蓝型油菜。"

图7

甘蓝型油菜BnGSTs的系统进化、Motif组成和基因结构分析"

图8

甘蓝型油菜BnGSTs的共线性关系分析"

图9

BnGSTs在低温胁迫下的表达模式分析"

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