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作物学报 ›› 2017, Vol. 43 ›› Issue (08): 1161-1169.doi: 10.3724/SP.J.1006.2017.01161
李敏1,2,于太飞1,2,徐兆师2,张双喜3,闵东红1,陈明2,马有志2,柴守诚1,*,郑炜君1,*
LI Min 1,2,YU Tai-Fei1,2,XU Zhao-Shi2,ZHANG Shuang-Xi 3,MIN Dong-Hong 1,CHEN Ming2,MA You-Zhi2,CHAI Shou-Cheng 1,*,ZHENG Wei-Jun 1,*
摘要：
植物的核因子Y(NF-Y)是由3个亚基A、B和C组成，在响应非生物胁迫过程中起着重要的作用。本研究以大豆铁丰8号为材料建立大豆cDNA文库，以pGBKT7-GmDi19-5为诱饵，通过酵母双杂交技术筛选大豆cDNA文库，获得了大豆NF-Y转录因子家族亚基C的一个成员，命名为GmNF-YCa。该基因全长为864 bp，编码287个氨基酸，属于NF-YC亚家族。GmNF-YCa分子量为31.6 kD，等电点为5.07亲水性蛋白，具有一个跨膜结构域，无信号肽。序列分析表明，NF-YC亚家族具有很高的保守性。GmNF-YCa基因启动子含有ARE、Box4、GATA-motif、Box I、ACE、ABRE和CAT-Box等胁迫和光响应元件。组织特异性分析表明，GmNF-YCa基因在种子萌发期表达量最高。实时定量结果表明，GmNF-YCa受蔗糖和甘露醇的诱导上调表达。使用农杆菌介导法将大豆GmNF-YCa基因导入拟南芥，并进行了功能分析。发芽率试验分析表明，GmNF-YCa的转基因提高了转基因拟南芥萌发期对渗透胁迫的耐性；改良了在蔗糖和甘露醇处理下转基因拟南芥的根系生长和侧根发育。
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